Strain number NIES-3374  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlorogonium capillatum Nozaki, M.M.Watanabe & Aizawa  
Former name  
Common name Green alga  
Locality (Date of collection) Osaka, Japan  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Suzaki, Toshinobu  
Isolator (Date of isolation) Suzaki, Toshinobu (1988-**-**)  
Identified by Suzaki, Toshinobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  11-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information act1 ( AB973441 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Planktonic ; Prey for protozoa (flagellates, ciliates, amoebids, heliozoans)  
Other strain no. Other strain no. : ATCC 50936  
Remarks Axenic 
Hoshina, R., Tsukii, Y., Harumoto, T., Suzaki, T. 2021 Characterization of a green Stentor with symbiotic algae growing in an extremely oligotrophic environment and storing large amounts of starch granules in its cytoplasm. Sci Rep, 11, 2865 (article ID).
Strain(s): 3374 
PubMed: 33536497
DOI: 10.1038/s41598-021-82416-9

Higuchi, S., Sugahara, F., Pascual-Anaya, J., Takagi, W., Oisi, Y., Kuratani, S. 2019 Inner ear development in cyclostomes and evolution of the vertebrate semicircular canals. Nature, 565, 347–350.
Strain(s): 3374 
PubMed: 30518864
DOI: 10.1038/s41586-018-0782-y

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Higuchi, R., Song, C., Hoshina, R., Suzaki, T. 2018 Endosymbiosis-related changes in ultrastructure and chemical composition of Chlorella variabilis (Archaeplastida, Chlorophyta) cell wall in Paramecium bursaria (Ciliophora, Oligohymenophorea). Eur. J. Protistol., 66, 149-155.
Keywords: Cell wall; Chlorella variabilis; GlycosaminoglycansParamecium bursaria; Symbiosis
Strain(s): 3374 
PubMed: 30286318
DOI: 10.1016/j.ejop.2018.09.003

Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. 2015 Phylogenetic analysis of NAP, an unconventional actin of the Volvocales. Plant Syst. Evol., 301, 1725-1733.
Keywords: Volvocales; Divergent actin
Strain(s): 3515465767173374 
DOI: 10.1007/s00606-014-1187-5

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Song, C., Suzaki, T. 2013 Improved preservation of organelies in Paramecium bursaria by freeze-substitution with glutaraldehyde and osmium tetroxide. J. Electr. Microsc. Technol. Med. Biol., 27, 1-8.
Keywords: Paramecium bursaria; endosymbiosis; cryofixation; ultrastructural preservation; freeze-substitution
Strain(s): 3374 

Sakaguchi, M., Suzuki, T., Khan, S. M. M. K. Hausmann, K. 2002 Food capture by kinetocysts in the heliozoon Raphidiophrys contractilis. Eur. J. Protistol., 37, 453-458.
Keywords: Exocytosis; Extrusome; Heliozoa; Kinetocyst
Strain(s): 24983374 
DOI: 10.1078/0932-4739-00847

Sakaguchi, M., Murakami, H., Suzaki, T. 2001 Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Protist, 152, 33-41.
Strain(s): 24973374 
PubMed: 11401035
DOI: 10.1078/1434-4610-00041

Sakaguchi, M., Suzuki, T. 1999 Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol., 35, 1-10.
Keywords: Actinophrys sol; Antibiotic; Heliozoa; Monoxenic Culture
Strain(s): 24973374 
DOI: 10.1016/S0932-4739(99)80050-9

Kinoshita, E., Suzaki, T., Sugiyama, M., Shigenaka, Y. 1995 Ultrastructure and rapid axopodial contraction of a heliozoan, Raphidiophyrys contractilis sp. nov. J. Eucaryot. Microbiol., 42, 283-288.
Keywords: Contraction; Heliozoa; microtubule pattern; new species; Raphidiophyrys; silicic scale; X-ray microanalysis
Strain(s): 24983374 
DOI: 10.1111/j.1550-7408.1995.tb01581.x

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