Strain number NIES-221  
Phylum Chlorophyta  
Class Prasinophyceae  
Scientific name Pterosperma cristatum Schiller  
Synonym  
Former name  
Common name  
Locality (Date of collection) Harima-Nada, Seto Inland Sea Japan (1983-02-**)  
Latitude / Longitude 34.661308 / 134.495801 
Habitat (Isolation source) Marine (Sediment)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-09-29)  
Identified by Inouye, Isao  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2ESM  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AJ010407 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Red tide  
Other strain no. Other strain no. : H-88-1  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic 
Movie  
Reference
Suda, S., Bhuiyan, M. A. H., Faria, D. G. 2013 Genetic diversity of Pyramimonas from Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales). J. Mar. Sci. Tech., 21, 285-296.
Keywords: Pyramimonas; nuclear SSU rDNA; genetic diversity; Ryukyu Archipelago
Strain(s): 2212512553392339333943396339733983399340134033405340734083411341334143415341634173418 
DOI: 10.6119/JMST-013-1220-16

Alamsjah, M. A., Hirao, S., Ishibashi, F., Oda, T., Fujita, Y. 2008 Algicidal activity of polyunsaturated fatty acids derived from Ulva fasciata and U. pertusa (Ulvaceae, Chlorophyta) on phytoplankton. J. Appl. Phycol., 20, 713-720.
Keywords: Algicidal compound; Red tide phytoplankton; Ulva fasciata; Ulva pertusa
Strain(s): 137221251252254359369381462486 
DOI: 10.1007/s10811-007-9257-5

Nakayama, T., Marin, B., Kranz, H. D., Surek, B., Huss, V. A. R., Inouye, I., Melkonian, M. 1998 The basal position of scaly green flagellates among the green algae (Chlorophyta) is revealed by analyses of nuclear-encoded SSU rRNA sequences. Protist, 149, 367-380.
Strain(s): 221251 
DOI: 10.1016/S1434-4610(98)70043-4

Marin, B., Melkonian, M. 1994 Flagellar hairs in prasinophytes (Chlorophyta): ultrastructure and distribution on the flagellar surface. J. Phycol., 30, 659-678.
Keywords: flagellar hairs; Mamiella; Mantoniella; Nephroselmis; Prasinophyceae; Pseudoscourfieldia; Pterosperma; Pyramimonas; Scherffelia; ultrastructure
Strain(s): 221483485 
DOI: 10.1111/j.0022-3646.1994.00659.x

Uye, S., Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107.
Strain(s): 1215221235251 

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