A big image opens when the thumbnail is clicked.(nies-0512.jpg)
Strain number NIES-512  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Pseudanabaena foetida Niiyama, Tuji & Ichise var. intermedia Tuji & Niiyama  
Synonym Phormidium tenue Gomont; Pseudanabaena galeata Böcher  
Former name Formerly identified as Pseudanabaena galeata, re-identified by Tuji & Niiyama 2016[2016 Oct]; Phormidium tenue  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Nagoya Castle, Nagoya, Aichi, Japan (1981-11-01)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pond water)  
History < Yamada, Naoki  
Isolator (Date of isolation) Yamada, Naoki (1985-05-22)  
Identified by Tuji, Akihiro; Niiyama, Yuko  
State of strain Subculture / Cryopreservation; Unialgal; Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CT  
Temperature:  20 (20) C
Light intensity:  21 (13-21) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  20 D (7 D)  
Gene information 16S rRNA ( AB042838 LC153790 LC314126 ) , rbcL ( LC314127 ) , pgmtc ( AB826230 ) , pgmtf ( AB826229 )  
Cell size (min - max) 2 - 8 μm  
Organization Filamentous 
Characteristics Offensive odor ; Test strain (Nagai, T., 2016) ; 2-MIB production (Iwase et al. 2010) ; Type strain (Tuji & Niiyama 2016)  
Other strain no. Other strain no. : PM-81A  
Remarks Unstable; Cryopreserved 
Suruzzaman, M., Cao, T., Lu, J., Wang, Y., Su, M., Yang, M. 2022 Evaluation of the MIB-producing potential based on real-time qPCR in drinking water reservoirs. Environ. Res., 204, 112308 (article ID).
Keywords: 2-Methylisoborneol (MIB); Taste & odor; Real time qPCR; MIB-Synthesis (mic) gene; Drinking water
Strain(s): 5124062406342374238 
PubMed: 34757030
DOI: 10.1016/j.envres.2021.112308

Lee, J. E., Yu, M. N., Yu, S., Byeon, M. 2022 Occurrence and phylogenetic analysis of Pseudanabaena sp. producing 2-methylisoborneol in drinking water source of South Korea. Environ. Microbiol. Rep., 14, 197-202.
Strain(s): 512 
PubMed: 34837342
DOI: 10.1111/1758-2229.13031

Devi, A., Chiu, Y.-T., Hsueh, H.-T., Lin, T.-F. 2021 Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Res., 188, 116478 (article ID).
Keywords: biomolecular; drinking water; database; geo; mic; monitoring; Taq-Man probes
Strain(s): 5124062406342374238 
PubMed: 33045635
DOI: 10.1016/j.watres.2020.116478

Shen, O., Wang, Q., Miao, H., Shimada, M., Utsumi, M., Lei, Z., Zhang, Z., Nishimura, O., Asada, Y., Fujimoto, N., Takanashi, H., Akiba, M., Shimizu, K. 2021 Temperature affects growth, geosmin/2-methylisoborneol production, and gene expression in two cyanobacterial species. Environ. Sci. Pollut. Res., 29, 12017–12026.
Keywords: Cyanobacteria; Temperature; Cell growth; Geosmin; 2-MIB; Gene expression
Strain(s): 512524 
PubMed: 34558048
DOI: 10.1007/s11356-021-16593-5

Muhetaer, G., Asaeda, T., Jayasanka, S. M. D. H., Baniya, M. B., Abeynayaka, H. D. L., Rashid, M. H., Yan, H. Y. 2020 Effects of light intensity and exposure period on the growth and stress responses of two cyanobacteria species: Pseudanabaena galeata and Microcystis aeruginosa. Water, 12, 407 (article ID).
Keywords: cyanobacterial growth; stress responses; Pseudanabaena galeata; Microcystis aeruginosa; oxidative stress; antioxidative enzymes
Strain(s): 111512 
PubMed: 32045866
DOI: 10.3390/w12020407

Kim, K., Yoon, Y., Cho, H., Hwang, S. J 2020 Molecular probes to evaluate the synthesis and production potential of an odorous compound (2-methylisoborneol) in cyanobacteria. Int. J. Environ. Res. Public Health, 17, 1933 (article ID).
Keywords: 2-Methylisoborneol (2-MIB); cyanobacteria; molecular probes; mibC; real-time PCR
Strain(s): 19512824 
PubMed: 32188031
DOI: 10.3390/ijerph17061933

Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Oliveira, E. D. C. de, Cunha, A. C. da, Silva, N. B. da, Castelo-Branco, R., Morais, J., Schneider, M. P. C., Faustino, S. M. M., Ramos, V., Vasconcelos, V. 2019 Morphological and molecular characterization of cyanobacterial isolates from the mouth of the Amazon River. Phytotaxa, 387, 269-288.
Keywords: Amazonia; Limnothrix; Polyphasic approach; Pseudanabaena; 16S rRNA phylogeny; Algae
Strain(s): 504512 
DOI: 10.11646/phytotaxa.387.4.1

Strain(s): 7533351280516421666167716961723 
DOI: 10.34566/jwwa.88.12_3

Abeynayaka, HDL., Asaeda, T., Rashid, MH. 2018 Effects of elevated pressure on Pseudanabaena galeata Böcher in varying light and dark environments. Environ. Sci. Pollut. Res., 25, 21224–21232.
Keywords: Cyanobacteria; Hydrostatic pressure; Pseudanabaena galeata Böcher; Prolonged darkness; Chlorophyll-a; Cell concentration
Strain(s): 512 
PubMed: 29779078
DOI: 10.1007/s11356-018-2218-5

Tuji, A., Niiyama, Y. 2018 Two new Pseudanabaena (Cyanobacteria, Synechococcales) species from Japan, Pseudanabaena cinerea and Pseudanabaena yagii, which produce 2-methylisoborneol. Phycol. Res., 66, 291-299.
Keywords: internal transcribed spacer region; Phormidiumtenue; Pseudanabaena foetida; Pseudanabaena subfoetida; rRNA operon; secondary structure
Strain(s): 5124059406240634064406542374238 
DOI: 10.1111/pre.12327

Tuji, A., Niiyama, Y. 2018 The taxonomy of Pseudanabaena(Cyanobacteria) and characters for Jjudging the production of odor compounds. Jpn. J. Water Treat. Biol., 54, 115-120 (in Japanese with English summary).
Keywords: 2-MIB; L/W ratio; Phormidium tenue; pigment; Pseudanabaena
Strain(s): 5124059406240634064406542374238 
DOI: 10.2521/jswtb.54.115

Nagai, T. 2017 Predicting herbicide mixture effects on multiple algal species using mixture toxicity models. Environ. Toxicol. Chem., 36, 2624-2630.
Keywords: Pesticide; Species sensitivity distribution; Mixture toxicology; Algae; Mode of action
Strain(s): 71487512797 
PubMed: 28316094
DOI: 10.1002/etc.3800

Nagai, T., Taya, K., Yoda, I. 2016 Comparative toxicity of 20 herbicides to 5 periphytic algae and the relationship with mode of action. Environ. Toxicol. Chem., 35, 368-375.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 71487512797 
PubMed: 26174500
DOI: 10.1002/etc.3150

Chiu, Y.-T., Yen, H.-K., Lin, T.-F. 2016 An alternativemethodtoquantify2-MIB producing cyanobacteriain in drinking water reservoirs: Method development and field applications. Environ. Res., 151, 618-627.
Keywords: Cyanobacteria; 2-methylisoborneol(2-MIB); 2-MIB-synthesis gene(mibC gene); Tasteandodor; Real-timePCR
Strain(s): 8151282490521042116 
PubMed: 27607443
DOI: 10.1016/j.envres.2016.08.034

Tuji, A., Niiyama, Y. 2016 The identity and phylogeny of Pseudanabaena Strain, NIES-512, producing 2-methylisoborneol (2-MIB). Bull. Natl. Mus. Nat. Sci., Ser. B, 42, 83-89.
Keywords: 2-MIB; Pseudanabaena foetida var. intermedia; Pseudanabaena foetida var. subfoetida; secondary structure
Strain(s): 5124059 

Kaya, K., Sano, T. 2016 Chapter 18. Cyanobacterial retinoids. In Handbook of cyanobacterial monitoring and cyanotoxin analysis, Eds. byMeriluoto, J., Spoof, L., Codd, G. A., John Wiley & Sons, Ltd. pp. 173-178.
Strain(s): 364445462045126101222 
DOI: 10.1002/9781119068761.ch18

Kakimoto, M., Ishikawa, T., Miyagi, A., Saito, K., Miyazaki, M., Asaeda, T., Yamaguchi, M., Uchiyama, H., Kawai-Yamada, M. 2014 Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeata. J. Plant Res., 171, 292-300.
Keywords: 2-MIB; Cyanobacteria; Gene expression; Metabolomics; Temperature-induction
Strain(s): 512 
PubMed: 24140001
DOI: 10.1016/j.jplph.2013.09.005

Nagai, T. 2014 河川付着藻類を用いた農薬の毒性試験マニュアル Ver. 1.0 In Research Project for Environmental Risk Assessment of Nutrients and Organochemicals, NIAES, 52 pp (in Japanese).
Strain(s): 71487512797 

Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 357173414487512797 
DOI: 10.1016/j.ecoenv.2013.04.020

Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Okuda, N., Nakano, S. 2012 Detection and identification of potentially toxic cyanobacteria: Ubiquitous distribution of Microcystis aeruginosa and Cuspidothrix issatschenkoi in Japanese lakes. Harmful Algae, 16, 49-57.
Keywords: Anatoxin-a; Automated rRNA intergenic spacer analysis (ARISA); Cuspidothrix issatschenkoi; Cyanotoxin; Microcystin; Microcystis aeruginosa
Strain(s): 304077102111230512843905928932937991164516691698172919502116 
DOI: 10.1016/j.hal.2012.01.003

Kaya, K., Shiraishi, F., Uchida, H., Sano, T. 2011 A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria. Biochim. Biophys. Acta-Gen. Subj., 1810, 414-419.
Keywords: 7-hydroxy retinoic acid; RAR agonistic activity; cyanobacteria; UV stability
Strain(s): 444546512610931 
PubMed: 21145941
DOI: 10.1016/j.bbagen.2010.11.009

Iwase, S., Abe, T. 2010 Identification and change in concentration of musty-odor compounds during frowth in blue-green algae. J. Sch. Marine Sci. Tech., Tokai Univ., 8, 27-33.
Strain(s): 5122119 

Ohko, Y., Nagao, Y., Okano, K., Sugiura, N., Fukuda, A., Yang, Y., Negishi, N., Takeuchi, M., Hanada, S. 2009 Prevention of Phormidium tenue biofilm formation by TiO2 photocatalysis. Microbes Environ., 24, 241-245.
Keywords: Phormidium tenue; cyanobacteria; TiO2 photocatalyst; anti-biofilm; UV light
Strain(s): 512 
DOI: 10.1264/jsme2.ME09106

Ishibashi, Y., Oikawa, E., Honda, H., Adachi, T., Nakatochi, M. 2007 Analyses of growth factors of taste and odor producing algae by a fuzzy neural network. Environ. Eng. Res. [Kankyô-kôgaku Kenkyû Ronbunshû], 44, 255-263 (in Japanese with English summary).
Keywords: musty odor; Phormidium tenue; Uroglena americana; fuzzy neural network; wind speed
Strain(s): 512 
DOI: 10.11532/proes1992.44.255

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19212228303437414445507378798081204208510512515592593610843846847209320962098210021022107210821182119212321242125212621282129213121323259327532763277 

Oshimi, M., Fujimoto, N., Ohnishi, A., Suzuki, M., Akiba, M., Kunikane, S. 2005 Isolation of bacteria lysing musty odor producing alga Phormidium tenue and its lytic characteristics. J. Jpn. Soc. Wat. Environ., 28, 379-384 (in Japanese with English summary).
Keywords: alga-lytic bacteria; musty odor; Phormidium tenue; Pseudomonas KA6
Strain(s): 96211227244298512817 
DOI: 10.2965/jswe.28.379

Zhou, S., Nakai, S., Hosomi, M., Sezaki, Y., Tominaga, M. 2004 Inhibition of cyanobacterial growth by allelopathy of reed. Jpn. J. Water Treat. Biol., 40, 23-28 (in Japanese with English summary).
Keywords: reed; Phormidium tenue; cyanobacteria; inhibition; allelopathy
Strain(s): 512 
DOI: 10.2521/jswtb.40.23

Oikawa, E. Ishibashi, Y., 2004 Species specificity of musty odor producing Phormidium tenue in Lake Kamafusa. Wat. Sci. Tech., 49, 41-46.
Keywords: 16S-rDNA sequence; 2-methylisoborneol; musty odor; Phormidium tenue phylogenetic tree
Strain(s): 213033417376512611 
PubMed: 15237605
DOI: 10.2166/wst.2004.0529

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary).
Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation
Strain(s): 35718796489512 

Oikawa, E., Ishibashi, Y., Abe, T., Umetsu, H. 2000 Phylogenetic classification of musty odor and/or toxic compounds producing cyanobacteria. Environ. Eng. Res., 37, 183-191 (in Japanese with English summary).
Keywords: cyanobacteria; musty odor; 16S ribosomal RNA; PCR; phylogenetic trees
Strain(s): 213033417376512 
DOI: 10.11532/proes1992.37.183

Nakai, S., Inoue, Y., Hosomi, M., Murakami, A. 1999 Growth inhibition of blue-green algae by allelopathic effects of macrophytes. Wat. Sci. Tech., 39, 47-53.
Keywords: allelopathy; blue-green algae; coexistence; growth inhibition; macrophyte; Myriophyllum spicatum
Strain(s): 7587512 
DOI: 10.1016/S0273-1223(99)00185-7

Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1995 Quantitative determination of cyanobacteria in mixed phytoplankton assemblages by an in vivo fluorimetric method. Anal. Chim. Acta, 302, 81-87.
Keywords: cyanobacteria; quantitative estimation; phycocyanin; waterblooms; Microcystis aeruginosa
Strain(s): 298512 
DOI: 10.1016/0003-2670(94)00425-L

Lee, T., Tsuzuki, M., Takeuchi, T., Yokoyama, K., Karube, I. 1994 In vivo fluorometric method for early detection of cyanobacterial waterblooms. J. Appl. Phycol., 6, 489-495.
Keywords: cyanobacteria; waterblooms; in vivo fluorescence; monitoring; Microcystis aeruginosa
Strain(s): 298512 

Oikawa, E., Ohnuma, T., Shimizu, A., Ishibashi, Y. 1994 Phylogenetic studies on musty odor producing cyanobacteria using the 16S ribosomal RNA [16S ribosômu RNA enki-hairetsu no hikaku ni yoru kabi-shû sansei ransô-rui no bunrui]. J. Jpn. Water Works Assoc. [Suidô-Kyôkai Zasshi], 63(12), 56-63 (in Japanese).
Keywords: musty odor; gene; cyanobacteria
Strain(s): 3676512 

Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1986 Studies on earthy-musty odor in natural water (3). Isolation of bacteria-free Phormidium tenue and the effect of associated bacteria on the growth of axenic P. tenue. Jpn. J. Water Poll. Res., 9, 379-385 (in Japanese with English summary).
Keywords: Phormidium tenue; axenic; growth; 2-methylisoborneol; bacteria interaction
Strain(s): 512 

Yamada, N., Aoyama, K., Yamada, M., Hamamura, N. 1985 Studies on earthy-musty odor in natural water (1). Growth characteristics and 2-methylisoborneol production of Phormidium tenue. Jpn. J. Water Poll. Res., 8, 515-521 (in Japanese with English summary).
Keywords: blue-green algae; Phormidium tenue; growth characteristics; headspace-GC analysis; 2-methylisoborneol
Strain(s): 512 

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