Strain number NIES-2842  
Phylum Heterokontophyta  
Class Bacillariophyceae  
Scientific name Skeletonema menzelii Guillard, Carpenter & Reimann  
Former name  
Common name Centric diatom  
Locality (Date of collection) Dokai Bay, Kitakyushu, Fukuoka, Japan (2008-09-10)  
Latitude / Longitude 33.88388888 / 130.78722222 
Habitat (Isolation source) Marine (Seawater)  
History < Yamada, Machiko  
Isolator (Date of isolation) Yamada, Machiko (2008-**-**)  
Identified by Yamada, Machiko  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  15 C
Light intensity:  3-10 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information 28S rRNA ( AB572830 ) , CO1 ( AB948163 )  
Cell size (min - max)  
Characteristics Planktonic  
Other strain no. Other strain no. : FDK222  
Yamada, M., Otsubo, M., Tsutsumi, Y., Mizota, C., Nakamura, Y., Takahashi, K., Iwataki M. 2017 Utility of mitochondrial-encoded cytochrome c oxidase I gene for phylogenetic analysis and species identification of the planktonic diatom genus Skeletonema. Phycol. Res., 65, 217-225.
Keywords: cox1; genetic marker; LSU rDNA; phylogenetictree; species delimitation; SSU rDNA
Strain(s): 16223283728382842 
DOI: 10.1111/pre.12179

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1891415177111522322523323426527427527627727827928028128228429332332433033334434534634734835035337237738839139540740840941341441746146246648753454855355655755855956056258758858959060360562262369569669769869970070170270370470570670770870971071171271371471571674176576676780583799710011002100310041005100610071009101110161017104410451046104713021303132413271330133913401349135313701375137613791383138413851386138713911392139313951398139914001401169917001730181318151816182618271831186218631864186518741963196419651974197519762142214321442145214721482300233123322351236323642365236923702376250625332534253525362537259026332668268926902691269326942696269727072716271727182720272227232725272627292730273127322770277127722773283928402841284228432844285928722878289028993391368936903691 

Kawachi, M., Kataoka, T., Sato, M., Noël, M.-H., Kuwata, A., Demura, M., Yamaguchi, H. 2016 Application of cryopreservation to genetic analyses of a photosynthetic picoeukaryote community. Gene, 576, 708-716.
Keywords: 18S rRNA gene; 454 pyros equencing; Cryopreservation; Photosynthetic picoeukaryotes
Strain(s): 76519662842 
PubMed: 26506442
DOI: 10.1016/j.gene.2015.10.033

Kaeriyama, H., Katsuki, E., Otsubo, M., Yamada, K., Ichimi, K., Tada, K., Harrison, P. J. 2011 Effects of temperature and irradiance on growth of strains belonging to seven Skeletonema species isolated from Dokai Bay, southern Japan. Eur. J. Phycol., 46, 113-124.
Keywords: Dokai Bay; irradiance; physiology; Skeletonema species; specific growth rate; temperature
Strain(s): 2837283828392841284228432844 
DOI: 10.1080/09670262.2011.565128

Yamada, M., Katsuki, E., Otsubo, M., Kawaguchi, M., Ichimi, K., Keriyama, H., Tada, K., Harrison, P. J. 2010 Species diversity of the genus Skeltonema (Bacillariophyceae) in the Industrial Harbor Dokai Bay, Japan. J. Oceanogr., 66, 755-771.
Keywords: Skeletonema; species diversity; isolation characteristics; Dokai Bay; LSU rDNA; SSU rDNA; SEM
Strain(s): 2837283828392841284228432844 
DOI: 10.1007/s10872-010-0062-4

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