Strain number NIES-2740  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Volvox kirkiorum H.Nozaki, H.Kawai-Toyooka & N.Isaka  
Synonym  
Former name  
Common name Green alga ; Volvocine alga  
Locality (Date of collection) Hajima, Gifu, Japan (2006-07-27)  
Latitude / Longitude 35.253 / 136.67444444 
Habitat (Isolation source) Freshwater  
History < Nozaki, Hisayoshi  
Isolator (Date of isolation) Nozaki, Hisayoshi (2006-07-28)  
Identified by Isaka, Nanako; Matsuzaki, Ryo; Nozaki, Hisayoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  AF-6VTAC  
Temperature:  20 C
Light intensity:  12-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-26S ( AB663324 ) , rbcL ( AB663322 ) , psbC ( AB663323 ) , MID ( LC699264 ) , act1 ( LC817973 )  
Cell size (min - max)  
Organization Colonial; Flagellate 
Characteristics Mixotrophic ; Osmotrophic ; Phototaxis ; Authentic strain (Isaka 2012) ; Homothallic ; Monoecious ; Oogamy ; Resting zygote forming  
Other strain no. Other collection strain no. : UTEX 2966
Other strain no. : 2006-728-2-B8  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Movie  
Reference
Yamagishi, S., Yamamoto, K., Takahashi, K., Kawai-Toyooka, H., Suzuki, S., Matsuzaki, R., Yamaguchi, H., Kawachi, M., Higashiyama, T., Nozaki, H. 2024 Evolutionary analysis of MID homologs during the transition from homothallic species to heterothallic species in Volvox sect. Volvox (Chlorophyceae). Phycol. Res., 72, 46-55.
Keywords: male gamete; mating system; minus dominance gene (MID); molecular evolution; sex; sperm packet; volvocine green algae
Strain(s): 28945646054154454556957257458265265365473285185285787289189213621363170817101859245725692735273627403382338337803782378638743984398639884021402543364432453545364537 
DOI: 10.1111/pre.12538

Nozaki, H., Matsuzaki, R., Shimotori, K., Ueki, N., Heman, W., Mahakham, W., Yamaguchi, H., Tanabe, Y., Kawachi, M. 2024 Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa. PLoS One, 19, e0310549 (article ID).
Strain(s): 543273627403874402140224258425943364337443446614662 
PubMed: 39312548
DOI: 10.1371/journal.pone.0310549

Nozaki, H., Mahakham, W., Heman, W., Matsuzaki, R., Kawachi, M. 2020 A new preferentially outcrossing monoicous species of Volvox sect. Volvox (Chlorophyta) from Thailand. PLoS One, 15, e0235622 (article ID).
Strain(s): 273627403382338338744021433644324433443444354436 
PubMed: 32614898
DOI: 10.1371/journal.pone.0235622

Isaka, N., Kawai-Toyooka, H., Matsuzaki, R., Nakada, T., Nozaki, H. 2012 Description of two new monoecious species of Volvox sect. Volvox (Volvocaceae, Chlorophyceae), based on comparative morphology and molecular phylogeny of cultured material. J. Phycol., 48, 759-767.
Keywords: Chlorophyceae; cultures; molecular phylogeny; morphology; taxonomy; Volvocaceae; Volvox ferrisii sp. nov.; Volvox kirkiorum sp. nov.; Volvox sect. Volvox
Strain(s): 54327362737273827392740 
PubMed: 27011093
DOI: 10.1111/j.1529-8817.2012.01142.x

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