Strain number NIES-2682  
Phylum Heterokontophyta  
Class Bolidophyceae  
Scientific name Triparma pacifica (Guillou & Chrétiennot-Dinet) Ichinomiya & Lopes dos Santos  
Synonym Bolidomonas pacifica L.Guillou & M.-J.Chrétiennot-Dinet  
Former name Bolidomonas pacifica L.Guillou & M.-J.Chrétiennot-Dinet  
Common name  
Locality (Date of collection) Equatorial Pacific Ocean (Depth -15m) (1994-11-11)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < RCC (2010);  
Isolator (Date of isolation) Vaulot, Daniel (1994-11-11)  
Identified by Guillou, L.  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESMMNK  
Temperature:  20 C
Light intensity:  24-35 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AF123595 ) , psbA ( EU851958 )  
Cell size (min - max) - 2 μm  
Characteristics Planktonic ; Authentic strain (Ichinomiya et al. 2016) ; Non-siliceous flagellated stage[2018 May]  
Other strain no. Other collection strain no. : RCC205; CCMP1866
Other strain no. : OLI 31 SE3  
Remarks Fragile species to transportation stresses; Read and agree "How to order 4.1" 
Kuwata, A., Yamada, K., Ichinomiya, M., Yoshikawa, S., Tragin, M., Vaulot, D., Lopes dos Santos, A. 2018 Bolidophyceae, a sister picoplanktonic group of diatoms – a review. Front. Mar. Sci., 5, 370 (article ID).
Keywords: bolidophyceae; parmales; diatoms; genetic diversity; mitosis; geographical distribution; seasonal dynamics and silicification
Strain(s): 25652682336933703371369937003701 
DOI: 10.3389/fmars.2018.00370

Ichinomiya, M., Lopes dos Santos, A., Gourvil, P., Yoshikawa, S., Kamiya, M., Ohki, K., Audic, S., De Vargas, C., Noël, M. H., Vaulot, D., Kuwata, A. 2016 Diversity and oceanic distribution of the Parmales (Bolidophyceae), a picoplanktonic group closely related to diatoms. ISME J., 10, 2419-2434.
Strain(s): 25652682336933703371369937003701 
PubMed: 27003244
DOI: 10.1038/ismej.2016.38

Kessenich, C. R., Ruck, E. C., Schurko, A. M., Wickett, N. J., Alverson, A. J. 2014 Transcriptomic insights into the life history of Bolidophytes, the sister lineage to diatoms. J. Phycol., 50, 977-983.
Keywords: Bolidomonas; diatoms; diplontic; haplodiplontic; life cycle; Parmales; transcriptome
Strain(s): 2682 
PubMed: 26988780
DOI: 10.1111/jpy.12222

Ichinomiya, M., Yoshikawa, S., Kmiya, M., Ohki, K., Takaichi, S., Kuwata, A. 2011 Isolation and characterization of Parmales (Heterokonta/Heterokontophyta/Stramenopiles) from the Oyashio region, Western North Pacific. J. Phycol., 47, 144-151.
Keywords: Bolidophyceae; diatoms; heterokonts; Parmales; PDMPO; phylogeny; stramenopiles; Triparma laevis
Strain(s): 25652682 
PubMed: 27021720
DOI: 10.1111/j.1529-8817.2010.00926.x

Guillou, L. 2011 Characterization of the Parmales: much more than the resolution of a taxonomic enigma. J. Phycol., 47, 2-4.
Strain(s): 2682336933703371 
DOI: 10.1111/j.1529-8817.2010.00951.x

Man-Aharonovich, D., Philosof, A., Kirkup, B. C., Le Gall, F., Yogev, T., Berman-Frank, I., Polz, M. F., Vaulot, D., Béjà, O. 2010 Diversity of active marine picoeukaryotes in the Eastern Mediterranean Sea unveiled using photosystem-II psbA transcripts. ISME J., 4, 1044-1052.
Keywords: picoeukaryotes; PPEs; psbA; transcripts; Mediterranean Sea; diversity
Strain(s): 258826822687 
PubMed: 20237514
DOI: 10.1038/ismej.2010.25

Not, F., Simon, N., Biegala, I. C., Vaulot, D. 2002 Application of fluorescent in situ hybridization coupled with tyramide signal amplification (FISH-TSA) to assess eukaryotic picoplankton composition. Aquat. Microb. Ecol., 28, 157-166.
Keywords: Fluorescent in situ hybridization; Tyramide signal amplification; Picoplankton; Eukaryotes; Coastal waters; Diversity
Strain(s): 26732682 
DOI: 10.3354/ame028157

Guillou, L., Moon-van der Staay, S. Y., Claustre, H., Partensky, F., Vaulot, D. 1999 Diversity and abundance of Bolidophyceae (Heterokonta) in two oceanic regions. Appl. Environ. Microbiol., 65, 4528-4536.
Strain(s): 2586258926822691 
PubMed: 10508085
DOI: 10.1128/AEM.65.10.4528-4536.1999

Guillou, L., Chrétiennot-Dinet, M. J., Medlin, L. K., Claustre, H., Loiseaux-de Goër, S., Vaulot, D. 1999 Bolidomonas: a new genus with two species belonging to a new algal class, the Bolidophyceae (Heterokonta). J. Phycol., 35, 368-381.
Keywords: Bolidophyceae; diatoms; Heterokonta; marine picoeukaryotes; oligotrophic ocean; Stramenopiles
Strain(s): 2682 
DOI: 10.1046/j.1529-8817.1999.3520368.x

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.