Strain number NIES-2666  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Kamigo Saitama, Japan (2004-08-08)  
Latitude / Longitude 36.2692 / 139.1331 
Habitat (Isolation source) Freshwater (Paddy water)  
History < Sekimoto, Hiroyuki  
Isolator (Date of isolation) Tsuchikane, Yuki (2004-08-10)  
Identified by Tsuchikane, Yuki  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  22 C
Light intensity:  11-14 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 5.8S-ITS2-28S ( AB625611 ) , 18S rRNA ( AB477426 ) , CpPI-kd01 ( AB558136 ) , CpPI-kd02 ( AB558137 ) , CpPI-kd03 ( AB558138 ) , CpPI-kd04 ( AB558139 ) , CpPI-kd05 ( AB558140 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Homothallic ; Isogamous  
Other strain no. Other strain no. : kodama20  
Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 53545859626364656826126664319432043214548455045514552 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 5859606162636465676869702612666 
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Kokubun, Y., Sekimoto, H. 2010 Characterization and molecular cloning of conjugation-regulating sex pheromones in homothallic Closterium. Plant Cell Physiol., 51, 1515-1523.
Keywords: Closterium; Conjugation; Homothallic; Homothallism; Sex pheromone; Sexual reproduction; Zygospore
Strain(s): 2666 
PubMed: 20656896
DOI: 10.1093/pcp/pcq110

Tsuchikane, Y., Sato, M., Ootaki, T., Kokubun, Y., Nozaki, H., Ito, M., Sekimoto, H. 2010 Sexual prosesses and phylogenetic relationship of a homothallic strain in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 46, 278-284.
Keywords: Closterium; conjugation; heterothallic; pheromone; sexual reproduction
Strain(s): 2666 
DOI: 10.1111/j.1529-8817.2010.00806.

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