A big image opens when the thumbnail is clicked.(nies-2565.jpg)
Strain number NIES-2565  
Phylum Heterokontophyta  
Class Bolidophyceae  
Scientific name Triparma laevis B.C.Booth f. inornata S.Konno, R.Ohira, C.Komuro, N.Harada, & R.W.Jordan  
Former name  
Common name  
Locality (Date of collection) Pacific Ocean (Oyashio region) (Depth -80m) Japan (2008-07-20)  
Latitude / Longitude 42.0 / 145.25 
Habitat (Isolation source) Marine (Seawater)  
History < Ichinomiya, Mutsuo  
Isolator (Date of isolation) Ichinomiya, Mutsuo (2008-07-27)  
Identified by Ichinomiya, Mutsuo  
State of strain Subculture; Unialgal; Axenic[2019 Jul]  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  5 C
Light intensity:  45-55 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Whole-genome ( BLQM00000000 ) , 16S rRNA ( LN735526 ) , 18S rRNA ( AB546639 ) , ITS-1-5.8S-ITS2 ( KR998385 ) , rbcL ( AB546640 ) , nadH1 ( KR998404 )  
Cell size (min - max)  
Characteristics Genome decoded strain (Kuwata et al. 2020) ; Siliceous, Non-flagellated stage[2018 May]  
Other strain no. Other strain no. : TOY-0807  
Remarks Axenic; Fragile species to transportation stresses; Fragile species to temperature changes; Read and agree "How to order 4.1" 
Kuwata, A., Saitoh, K., Nakamura, Y., Ishinomiya, M., Sato, N. 2020 Draft whole-genome sequence of Triparma laevis f. inornata (Parmales, Bolidophyceae), isolated from the Oyashio region, Western North Pacific Ocean. Microbiol. Resour. Ann.., 9, e00367-20 (article ID).
Strain(s): 2565 
PubMed: 32817146
DOI: 10.1128/MRA.00367-20

Yamada, K., Katsura, H., Noël, M.-H., Ichinomiya, M., Kuwata, A., Sato, S., Yoshikawa, S. 2019 Ontogenetic analysis of siliceous cell wall formation in Triparma laevis f. inornata (Parmales, Stramenopiles). J. Phycol., 55, 196-203.
Keywords: annulus; diatom; pattern center; scale; sternum
Strain(s): 2565 
PubMed: 30320892
DOI: 10.1111/jpy.12800

Kuwata, A., Yamada, K., Ichinomiya, M., Yoshikawa, S., Tragin, M., Vaulot, D., Lopes dos Santos, A. 2018 Bolidophyceae, a sister picoplanktonic group of diatoms – a review. Front. Mar. Sci., 5, 370 (article ID).
Keywords: bolidophyceae; parmales; diatoms; genetic diversity; mitosis; geographical distribution; seasonal dynamics and silicification
Strain(s): 25652682336933703371369937003701 
DOI: 10.3389/fmars.2018.00370

Yamada, K., Nagasato, C., Motomura, T., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K., Yoshikawa, S. 2017 Mitotic spindle formation in Triparma laevis NIES-2565 (Parmales, Heterokontophyta). Protoplasma, 254, 461-471.
Keywords: Central spindle; Centriole; Diatom; Extranuclear spindle; Mitosis; Nuclear envelope
Strain(s): 2565 
PubMed: 27048177
DOI: 10.1007/s00709-016-0967-x

Ichinomiya, M., Lopes dos Santos, A., Gourvil, P., Yoshikawa, S., Kamiya, M., Ohki, K., Audic, S., De Vargas, C., Noël, M. H., Vaulot, D., Kuwata, A. 2016 Diversity and oceanic distribution of the Parmales (Bolidophyceae), a picoplanktonic group closely related to diatoms. ISME J., 10, 2419-2434.
Strain(s): 25652682336933703371369937003701 
PubMed: 27003244
DOI: 10.1038/ismej.2016.38

Tajima, N., Saitoh, K., Sato, S., Maruyama, F., Ichinomiya, M.,Yoshikawa, S., Kurokawa, K., Ohta, H., Tabata, S., Kuwata, A., Sato, N. 2016 Sequencing and analysis of the complete organellar genomes of Parmales, a closely related group to Bacillariophyta (diatoms). Curr. Genet., 62, 887-896.
Keywords: Genome sequencing; Heterokonta; Mitochondrial genome; Parmales; Plastid genome; Triparma laevis
Strain(s): 2565 
PubMed: 27091756
DOI: 10.1007/s00294-016-0598-y

Yamada, K., Yoshikawa, S., Ohki, K., Ichinomiya, M., Kuwata, K., Motomura, T., Nagasato, C. 2016 Ultrastructural analysis of siliceous cell wall regeneration in the stramenopile Triparma laevis (Parmales, Bolidophyceae). Phycologia, 55, 602-609.
Keywords: Cell wall regeneration; Chloroplast endoplasmic reticulum; Exocytosis; Silica biomineralization; Silica deposition vesicle
Strain(s): 2565 
DOI: 10.2216/16-32.1

Decelle, J., Romac, S., Stern, R. F., Bendif, E., Zingone, A., Audic, S., Guiry, M. D., Guillou, L., Tessier, D., Le Gall, F., Gourvil, P., Dos Santos, A. L., Probert, I., Vaulot, D., de Vargas, C., Christen, R. 2015 PhytoREF: a reference database of the plastidial 16S rRNAgene of photosynthetic eukaryotes with curated taxonomy. Mol. Ecol. Resour., 15, 1435-1445.
Keywords: high-throughput sequencing; metabarcoding; photosynthesis; phytoplankton; plastidial 16S rRNA gene; protists
Strain(s): 7637642565 
PubMed: 25740460
DOI: 10.1111/1755-0998.12401

Yamada, K., Yoshikawa, S., Ichinomiya, M., Kuwata, A., Kamiya, M., Ohki, K. 2014 Effects of silicon-limitation on growth and morphology of Triparma laevis NIES-2565 (Parmales, Heterokontophyta). PLoS One, 9, e103289 (article ID).
Strain(s): 2565 
PubMed: 25054645
DOI: 10.1371/journal.pone.0103289

Ichinomiya, M., Nakamachi, M., Shimizu, Y., Kuwata, A. 2013 Growth characteristics and vertical distribution of Triparma laevis (Parmales) during summer in the Oyashio region, western North Pacific. Aquat. Microb. Ecol., 68, 107-116.
Keywords: Triparma laevis; Parmales; Vertical distribution; Growth characteristics; Oyashio region
Strain(s): 2565 
DOI: 10.3354/ame01606

Ichinomiya, M., Yoshikawa, S., Kmiya, M., Ohki, K., Takaichi, S., Kuwata, A. 2011 Isolation and characterization of Parmales (Heterokonta/Heterokontophyta/Stramenopiles) from the Oyashio region, Western North Pacific. J. Phycol., 47, 144-151.
Keywords: Bolidophyceae; diatoms; heterokonts; Parmales; PDMPO; phylogeny; stramenopiles; Triparma laevis
Strain(s): 25652682 
PubMed: 27021720
DOI: 10.1111/j.1529-8817.2010.00926.x

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