| Strain number | NIES-2540 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Trebouxiophyceae | |||
| Scientific name | Chlorella variabilis Shihira & Krauss | |||
| Synonym | Chlorella paramecii Loefer 1936 | |||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | Japan | |||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | (Paramecium bursaria F36) | |||
| History | < Hoshina, Ryo | |||
| Isolator (Date of isolation) | Kamako, Shin-ichiro (2002-**-**) | |||
| Identified by | Hoshina, Ryo | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar] | |||
|
Culture condition (Preculture condition) |
Medium:
CYT
;
HUT
Temperature: 20 C Light intensity: 4-10 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 1 M |
|||
| Gene information | 16S rRNA ( AB208588 ) , 18S-ITS1-5.8S-ITS2 ( AB162914 ) , rbcL ( AB260900 ) , psaA ( AB260914 ) | |||
| Cell size (min - max) | 5 - 8 μm | |||
| Organization | Unicellular | |||
| Characteristics | Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid) | |||
| Other strain no. | Other strain no. : F36-ZK | |||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
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Toyoshima, H., Miyata, A., Yoshida, R., Ishige, T., Takaichi, S., Kawasaki, S.
2021
Distribution of the water-soluble astaxanthin binding carotenoprotein (AstaP) in Scenedesmaceae.
Mar. Drugs,
19,
349 (article ID).
Keywords: astaxanthin; Scenedesmus; Coelastrella; carotenoprotein; astaxanthin-binding protein Strain(s): 2238, 2540 PubMed: 34203079 DOI: 10.3390/md19060349 Agarkova, I. V., Lane, L. C., Dunigan, D. D., Quispe, C. F., Duncan, G. A., Milrot, E., Minsky, A., Esmael, A., Ghosh, J. F., Van Etten, J. L. 2021 Identification of a chlorovirus PBCV-1 protein involved in degrading the host cell wall during virus infection. Viruses-Basel, 13, 782 (article ID). Keywords: Chlorella; Phycodnaviridae; chlorovirus; PBCV-1; algal lytic activity; cell wall degrading activity; alginate-like lyase Strain(s): 2540, 2541 PubMed: 33924931 DOI: 10.3390/v13050782 Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82. Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine Strain(s): 18, 35, 242, 691, 735, 1271, 1278, 2185, 2187, 2235, 2261, 2540, 2572, 3983 Quispe, C. F., Sonderman, O., Khasin, M., Riekhof, W. R., Van Etten, J. L., Nickerson, K. W. 2016 Comparative genomics, transcriptomics, and physiology distinguish symbiotic from free-living Chlorella strains. Algal Res., 18, 332-340. Keywords: Chlorella variabilis; Alga-paramecium symbiosis; Algal nitrogen metabolism; Amino acid transporters Strain(s): 2540, 2541 DOI: 10.1016/j.algal.2016.06.001 Hoshina, R., Iwataki, M., Imamura, N. 2010 Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycol. Res., 58, 188-201. Keywords: Chlorella variabilis; ITS2; Micractinium reisseri; Paramecium bursaria; species concept Strain(s): 2540, 2542 DOI: 10.1111/j.1440-1835.2010.00579.x Kato, Y., Imamura, N., 2008 Effect of calcium ion on uptake of amino acids by symbiotic Chlorella F36-ZK isolated from Japanese Paramecium bursaria. Plant Sci., 174, 88-96. Keywords: Paramecium bursaria; Chlorella; Calcium; Divalent cation; Amino acid transport; Symbiosis Strain(s): 227, 2157, 2540 DOI: 10.1016/j.plantsci.2007.10.001 Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486. Keywords: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella Strain(s): 227, 2540, 2541 DOI: 10.1016/j.plantsci.2005.09.018 Hoshina, R., Kato, Y., Kamako, S., Imamura, N. 2005 Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg. Plant Biol., 7, 526-532. Keywords: 18S rDNA; "American"; "European"; Chlorella; ITS; Paramecium bursaria; Phylogeny Strain(s): 2540, 2541 DOI: 10.1055/s-2005-865912 Hoshina, R., Kamako, S.-I., Imamura, N. 2004 Phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg from Japan. Plant Biol., 6, 447-453. Keywords: 18S rDNA; Chlorella; endosymbiont; ITS; Paramecium bursaria; phylogeny Strain(s): 2540, 2541 DOI: 10.1055/s-2004-820888 |
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