| Strain number | NIES-2540 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Trebouxiophyceae | |||
| Scientific name | Chlorella variabilis Shihira & Krauss | |||
| Synonym | Chlorella paramecii Loefer 1936 | |||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | Japan | |||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | (Paramecium bursaria F36) | |||
| History | < Hoshina, Ryo | |||
| Isolator (Date of isolation) | Kamako, Shin-ichiro (2002-**-**) | |||
| Identified by | Hoshina, Ryo | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2018 Mar] | |||
|
Culture condition (Preculture condition) |
Medium:
CYT
;
HUT
Temperature: 20 C Light intensity: 4-10 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 1 M |
|||
| Gene information | 16S rRNA ( AB208588 ) , 18S-ITS1-5.8S-ITS2 ( AB162914 ) , rbcL ( AB260900 ) , psaA ( AB260914 ) | |||
| Cell size (min - max) | 5 - 8 μm | |||
| Organization | Unicellular | |||
| Characteristics | Symbiotic (Paramecium bursaria) ; Auxotrophic (Amino acid) | |||
| Other strain no. | Other strain no. : F36-ZK | |||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
|---|
|
Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M.
2018
Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.—
Microb. Resour. Syst.,
34,
73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine Strain(s): 18, 35, 242, 691, 735, 1271, 1278, 2185, 2187, 2235, 2261, 2540, 2572, 3983 Quispe, C. F., Sonderman, O., Khasin, M., Riekhof, W. R., Van Etten, J. L., Nickerson, K. W. 2016 Comparative genomics, transcriptomics, and physiology distinguish symbiotic from free-living Chlorella strains. Algal Res., 18, 332-340. Keywords: Chlorella variabilis; Alga-paramecium symbiosis; Algal nitrogen metabolism; Amino acid transporters Strain(s): 2540, 2541 DOI: 10.1016/j.algal.2016.06.001 Hoshina, R., Iwataki, M., Imamura, N. 2010 Chlorella variabilis and Micractinium reisseri sp. nov. (Chlorellaceae, Trebouxiophyceae): Redescription of the endosymbiotic green algae of Paramecium bursaria (Peniculia, Oligohymenophorea) in the 120th year. Phycol. Res., 58, 188-201. Keywords: Chlorella variabilis; ITS2; Micractinium reisseri; Paramecium bursaria; species concept Strain(s): 2540, 2542 DOI: 10.1111/j.1440-1835.2010.00579.x Kato, Y., Imamura, N., 2008 Effect of calcium ion on uptake of amino acids by symbiotic Chlorella F36-ZK isolated from Japanese Paramecium bursaria. Plant Sci., 174, 88-96. Keywords: Paramecium bursaria; Chlorella; Calcium; Divalent cation; Amino acid transport; Symbiosis Strain(s): 227, 2157, 2540 DOI: 10.1016/j.plantsci.2007.10.001 Kato, Y., Ueno, S., Imamura, N. 2006 Studies on the nitrogen utilization of endosymbiotic algae isolated from Japanese Paramecium bursaria. Plant Sci., 170, 481-486. Keywords: Endosymbiont; Paramecium bursaria; Nitrogen utilization; Chlorella Strain(s): 227, 2540, 2541 DOI: 10.1016/j.plantsci.2005.09.018 Hoshina, R., Kato, Y., Kamako, S., Imamura, N. 2005 Genetic evidence of "American" and "European" type symbiotic algae of Paramecium bursaria Ehrenberg. Plant Biol., 7, 526-532. Keywords: 18S rDNA; "American"; "European"; Chlorella; ITS; Paramecium bursaria; Phylogeny Strain(s): 2540, 2541 DOI: 10.1055/s-2005-865912 Hoshina, R., Kamako, S.-I., Imamura, N. 2004 Phylogenetic position of endosymbiotic green algae in Paramecium bursaria Ehrenberg from Japan. Plant Biol., 6, 447-453. Keywords: 18S rDNA; Chlorella; endosymbiont; ITS; Paramecium bursaria; phylogeny Strain(s): 2540, 2541 DOI: 10.1055/s-2004-820888 |
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