Strain number NIES-2463  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii P.A.Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) Kokubumuke-cho, Kirishima, Kagoshima, Japan (2006-03-26)  
Latitude / Longitude 31.75 / 130.76666666 
Habitat (Isolation source) Freshwater (Soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2008-11-27)  
Identified by Nakada, Takashi  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  AF-6  
Temperature:  20 C
Light intensity:  7-13 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB701555 AB511836 ) , ITS1-5.8S-ITS2 ( AB511842 ) , rbcL ( AB511846 ) , FUS1 ( AB900980 ) , NIT1 ( AB900988 ) , NIT2 ( AB900996 )  
Cell size (min - max) 8 - 12 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Heterothallic ; Dioecious ; Isogamous ; Mating type (+)  
Other strain no. Other strain no. : KkS0801B1  
Remarks Cryopreserved; Axenic 
Takada, S., Tanaka, Y., Kumagai, K., Kobayashi, K., Hokura, A., Ogra, Y. 2022 Formation of biogenic tellurium nanorods in unicellular green alga Chlamydomonas reinhardtii. Metallomics, 14, mfac089 (article ID).
Keywords: tellurium; biogenic nanorod; Chlamydomonas reinhardtii; single-particle ICP-MS; electron microscopy; bioremediation
Strain(s): 2463 
PubMed: 36367503
DOI: 10.1093/mtomcs/mfac089

Nitta, N. et al. 2020 Raman image-activated cell sorting. Nat. Commun., 11, 3452 (article ID).
Strain(s): 48229324633640 
PubMed: 32651381
DOI: 10.1038/s41467-020-17285-3

Lee, S., Thio, S. K., Park, S.-Y., Bae, S. 2019 An automated 3D-printed smartphone platform integrated withoptoelectrowetting (OEW) microfluidic chip for on-site monitoring of viablealgae in water. Harmful Algae, 88, 101638 (article ID).
Keywords: Algae quantification; In-situ assessment; Viable algae; 3D-printed smartphone platform; Optoelectorwetting
Strain(s): 8432463 
PubMed: 31582154
DOI: 10.1016/j.hal.2019.101638

Mao, F., He, Y., Gin, KY. 2018 Evaluating the joint toxicity of two benzophenone-type UV filters on the Ggreen alga Chlamydomonas reinhardtii with response surface methodology. Toxics, 6, 8 (article ID).
Keywords: Chlamydomonas reinhardtii; benzophenone-1; benzophenone-3; joint toxicity; response surface methodology
Strain(s): 2463 
PubMed: 29320457
DOI: 10.3390/toxics6010008

Mao, F., He, Y., Kushmaro, A., Gin, KY. 2017 Effects of benzophenone-3 on the green alga Chlamydomonas reinhardtii and the cyanobacterium Microcystis aeruginosa. Aquat. Toxicol., 193, 1-8.
Keywords: Benzophenone-3; Toxicity; Green algae; Cyanobacteria; Photosynthesis; Uptake
Strain(s): 8432463 
PubMed: 28992446
DOI: 10.1016/j.aquatox.2017.09.029

Nakada, T., Tsuchida, Y., Arakawa, K., Ito, T., Tomita, M. 2014 Hybridization between Japanese and North American Chlamydomonas reinhardtii (Volvocales, Chlorophyceae). Phycol. Res., 62, 232-236.
Keywords: biogeography; Chlamydomonas reinhardtii; Chlorophyceae; mating population; species
Strain(s): 246324643378337933803381 
DOI: 10.1111/pre.12061

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Nakada, T., Shinkawa, H., Ito, T., Tomita, M. 2010 Recharacterization of Chlamydomonas reinhardtii and its relatives with new isolates from Japan. J. Plant Res., 123, 67-78.
Keywords: Chlamydomonas reinhardtii; Chlorophyceae; distribution; phycology; species; taxonomy
Strain(s): 17081858246224632464 
PubMed: 19882207
DOI: 10.1007/s10265-009-0266-0

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