Strain number NIES-447  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Gloeomonas anomalipyrenoides Nakada, Matsuzaki & Nozaki  
Former name Chloromonas insignis (Anachin) Gerloff & Ettl  
Common name Green alga  
Locality (Date of collection) Lake Kasumigaura, Tsuchiura, Ibaraki, Japan (1983-08-22)  
Latitude / Longitude 36.081204 / 140.214193 
Habitat (Isolation source) Freshwater (Lake water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-08-24)  
Identified by Nakada, Takashi (Reidentify)  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  20 C
Light intensity:  3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  2 M  
Gene information 18S rRNA ( AB504776 AB624567 ) , 18S-ITS1-5.8S-ITS2-28S ( AB971362 ) , ITS2 ( AB624578 ) , atpB ( AB084313 ) , psaA ( AB624593 ) , psaB ( AB084348 ) , rbcL ( AB022226 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Planktonic ; Authentic strain (Nakada et al. 2015)  
Other strain no. Other strain no. : Kas-8  
Remarks Axenic 
Watanabe, S., Nozaki, H., Nakada, T., Lewis, L. A. 2016 Comparative ultrastructural analysis of Chloromonas and Gloeomonas (Chlorophyceae): tracing the origin of Gloeomonas-specific basal apparatus traits. Phycologia, 55, 585-601.
Keywords: Chloromonadinia; Coarsely striated proximal fiber (CSPF); Distal connecting fiber-associated layered structure (DFLS); Gloeomonas rubrifilum comb. nov.; Ixipapillifera; Morphological novelty
Strain(s): 447464 
DOI: 10.2216/15-147.1

Nakada, T., Matsuzaki, R., Krienitz, L., Tomiwa, M., Nozaki, H. 2015 Taxonomic reassessment of strains formerly classified as Chloromonas insignis (Volvocales, Chlorophyceae) and description of Gloeomonas anomalipyrenoides sp. nov. Acta Phytotax. Geobot., 66, 23-33.
Keywords: 18S rRNA; Chloromonas; culture strain; Gloeomonas; light microscopy; new species; phycology; phylogeny
Strain(s): 447364036413642 
DOI: 10.18942/apg.KJ00009868503

Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85.
Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales
Strain(s): 447 
DOI: 10.2216/11-18.1

Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201.
Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales
Strain(s): 447464 
DOI: 10.1111/j.1529-8817.2009.00773.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96123144213418421425428432439446447456474522564566567569571574582653732851858861875882883968 
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO2-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372.
Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase
Strain(s): 447 
DOI: 10.1007/s004250050571

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