Strain Data

Strain number		NIES-447
Phylum		Chlorophyta
Class		Chlorophyceae
Scientific name		Gloeomonas anomalipyrenoides Nakada, Matsuzaki & Nozaki
Synonym
Former name		Chloromonas insignis (Anachin) Gerloff & Ettl
Common name		Green alga
Locality (Date of collection)		Lake Kasumigaura, Tsuchiura, Ibaraki, Japan (1983-08-22)
Latitude / Longitude		36.081204 / 140.214193
Habitat (Isolation source)		Freshwater (Lake water)
History		< Suda, Shoichiro
Isolator (Date of isolation)		Suda, Shoichiro (1983-08-24)
Identified by		Nakada, Takashi (Reidentify)
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 20 C Light intensity: 3 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA ( AB504776 AB624567 ) , 18S-ITS1-5.8S-ITS2-28S ( AB971362 ) , ITS2 ( AB624578 ) , atpB ( AB084313 ) , psaA ( AB624593 ) , psaB ( AB084348 ) , rbcL ( AB022226 )
Cell size (min - max)
Organization		Unicellular; Flagellate
Characteristics		Planktonic ; Authentic strain (Nakada et al. 2015)
Other strain no.		Other strain no. : Kas-8
Remarks		Axenic
Movie

Reference
Watanabe, S., Nozaki, H., Nakada, T., Lewis, L. A. 2016 Comparative ultrastructural analysis of Chloromonas and Gloeomonas (Chlorophyceae): tracing the origin of Gloeomonas-specific basal apparatus traits. Phycologia, 55, 585-601. Keywords: Chloromonadinia; Coarsely striated proximal fiber (CSPF); Distal connecting fiber-associated layered structure (DFLS); Gloeomonas rubrifilum comb. nov.; Ixipapillifera; Morphological novelty Strain(s): 447, 464 DOI: 10.2216/15-147.1 Nakada, T., Matsuzaki, R., Krienitz, L., Tomiwa, M., Nozaki, H. 2015 Taxonomic reassessment of strains formerly classified as Chloromonas insignis (Volvocales, Chlorophyceae) and description of Gloeomonas anomalipyrenoides sp. nov. Acta Phytotax. Geobot., 66, 23-33. Keywords: 18S rRNA; Chloromonas; culture strain; Gloeomonas; light microscopy; new species; phycology; phylogeny Strain(s): 447, 3640, 3641, 3642 DOI: 10.18942/apg.KJ00009868503 Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85. Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales Strain(s): 447 DOI: 10.2216/11-18.1 Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201. Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales Strain(s): 447, 464 DOI: 10.1111/j.1529-8817.2009.00773.x Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430. Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968 PubMed: 12355262 DOI: 10.1007/s00239-002-2338-9 Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO₂-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372. Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase Strain(s): 447 DOI: 10.1007/s004250050571

Reference

Watanabe, S., Nozaki, H., Nakada, T., Lewis, L. A. 2016 Comparative ultrastructural analysis of Chloromonas and Gloeomonas (Chlorophyceae): tracing the origin of Gloeomonas-specific basal apparatus traits. Phycologia, 55, 585-601.
Keywords: Chloromonadinia; Coarsely striated proximal fiber (CSPF); Distal connecting fiber-associated layered structure (DFLS); Gloeomonas rubrifilum comb. nov.; Ixipapillifera; Morphological novelty
Strain(s): 447, 464
DOI: 10.2216/15-147.1

Nakada, T., Matsuzaki, R., Krienitz, L., Tomiwa, M., Nozaki, H. 2015 Taxonomic reassessment of strains formerly classified as Chloromonas insignis (Volvocales, Chlorophyceae) and description of Gloeomonas anomalipyrenoides sp. nov. Acta Phytotax. Geobot., 66, 23-33.
Keywords: 18S rRNA; Chloromonas; culture strain; Gloeomonas; light microscopy; new species; phycology; phylogeny
Strain(s): 447, 3640, 3641, 3642
DOI: 10.18942/apg.KJ00009868503

Matsuzaki, R., Hara, Y., Nozaki, H. 2012 A taxonomic revision of Chloromonas reticulata (Volvocales, Chlorophyceae), the type species of the genus Chloromonas, based on multigene phylogeny and comparative light and electron microscopy. Phycologia, 51, 74-85.
Keywords: Chloromonadinia; Chloromonas; Chloromonas chlorococcoides comb. nov.; Chloromonas lineage; Chloromonas typhlos comb. nov.; Molecular phylogeny; Morphology; Taxonomy; Ultrastructure; Volvocales
Strain(s): 447
DOI: 10.2216/11-18.1

Nozaki, H., Nakada, T., Watanabe, S. 2010 Evolutionary origin of Gloeomonas (Volvocales, Chlorophyceae), based on ultrastructure of chloroplasts and molecular phylogeny. J. Phycol., 46, 195-201.
Keywords: Chloromonadinia; Chloromonas; evolution; Gloeomonas; molecular phylogeny; pyrenoid; ultrastructure; Volvocales
Strain(s): 447, 464
DOI: 10.1111/j.1529-8817.2009.00773.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Nozaki, H., Onishi, K., Morita, E. 2002 Differences in pyrenoid morphology are correlated with differences in the rbcL genes of members of the Chloromonas lineage (Volvocales, Chlorophyceae). J. Mol. Evol., 55, 414-430.
Keywords: ATP synthase β-subunit (atpB) gene; Chloromonas; gene evolution; large subunit of Rubisco (rbcL) gene; morphological evolution; P700 chlorophyll a-apoprotein A2 (psaB) gene; pyrenoid; Volvocales
Strain(s): 96, 123, 144, 213, 418, 421, 425, 428, 432, 439, 446, 447, 456, 474, 522, 564, 566, 567, 569, 571, 574, 582, 653, 732, 851, 858, 861, 875, 882, 883, 968
PubMed: 12355262
DOI: 10.1007/s00239-002-2338-9

Morita, E., Abe, T., Tsuzuki, M., Fujiwara, S., Sato, N., Hirata, A., Sonoike, K., Nozaki, H. 1999 Role of pyrenoids in the CO₂-concentrating mechanism: comparative morphology, physiology and molecular phylogenetic analysis of closely related strains of Chlamydomonas and Chloromonas (Volvocales). Planta, 208, 365-372.
Keywords: Chlamydomonas; Chloromonas; CO2-concentrating mechanism; molecular phylogeny; pyrenoid; ribulose-1,5-bisphosphate carboxylase/oxygenase
Strain(s): 447
DOI: 10.1007/s004250050571

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