Strain number NIES-2498  
Phylum Heliozoa  
Class Centrohelea  
Scientific name Raphidiophrys contractilis Kinoshita et al.  
Former name  
Common name  
Locality (Date of collection) Shukkeien Park, Naka-ku, Hiroshima, Hiroshima, Japan (1995-**-**)  
Latitude / Longitude 34.4 / 132.46722222 
Habitat (Isolation source) Brackish water  
History < Suzaki, Toshinobu  
Isolator (Date of isolation) Suzaki, Toshinobu (1995-**-**)  
Identified by Suzaki, Toshinobu  
State of strain Subculture; Axenic[2021 Sept]; Mixed (Chlorogonium capillatum NIES-3374 as food)  
Culture condition
(Preculture condition)
Medium:  Helio  
Temperature:  20 C
Light intensity:  4-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information  
Cell size (min - max)  
Other strain no. Other strain no. : HSR  
Remarks Axenic 
Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Khan, S. M. M. K., Yoshimura, C., Arikawa, M., Omura, G., Nishiyama, S., Suetomo, Y., Kakuta, S., Suzaki, T. 2006 Axopodial degradation in the heliozoon Raphidiophrys contractilis: a novel bioassay system for detecting heavy metal toxicity in an aquatic environment. Environ. Sci., 13, 193-200.
Strain(s): 2498 
PubMed: 17095991

Khan, S. M. M. K., Arikawa, M., Suzaki, T. 2005 Toxic effect of heavy metal ions on the axopodia of the heliozoon Raphidiophrys contractilis. Jpn. J. Protozool., 38, 44-45.
Strain(s): 2498 

Khan, S. M. M. K., Arikawa, M., Omura, G., Suetomo, Y., Kakuta, S., Suzaki, T. 2003 Axopodial contraction in the heliozoon Raphidiophrys contractilis requires extracellular Ca2+. Zool. Sci., 20, 1367-1372.
Keywords: contraction; microtubules; contractile tubules; heliozoa; calcium
Strain(s): 2498 
DOI: 10.2108/zsj.20.1367

Sakaguchi, M., Suzuki, T., Khan, S. M. M. K. Hausmann, K. 2002 Food capture by kinetocysts in the heliozoon Raphidiophrys contractilis. Eur. J. Protistol., 37, 453-458.
Keywords: Exocytosis; Extrusome; Heliozoa; Kinetocyst
Strain(s): 24983374 
DOI: 10.1078/0932-4739-00847

Kinoshita, E., Suzaki, T., Sugiyama, M., Shigenaka, Y. 1995 Ultrastructure and rapid axopodial contraction of a heliozoan, Raphidiophyrys contractilis sp. nov. J. Eucaryot. Microbiol., 42, 283-288.
Keywords: Contraction; Heliozoa; microtubule pattern; new species; Raphidiophyrys; silicic scale; X-ray microanalysis
Strain(s): 24983374 
DOI: 10.1111/j.1550-7408.1995.tb01581.x

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