Strain number NIES-2497  
Phylum Stramenopila incertae sedis  
Class Nucleohelea  
Scientific name Actinophrys sol Ehrenberg  
Former name  
Common name  
Locality (Date of collection) Shukkeien Park, Naka-ku, Hiroshima, Hiroshima, Japan (2001-**-**)  
Latitude / Longitude 34.4 / 132.46722222 
Habitat (Isolation source) Brackish water  
History < Suzaki, Toshinobu  
Isolator (Date of isolation) Suzaki, Toshinobu (2001-**-**)  
Identified by Suzaki, Toshinobu  
State of strain Subculture; Axenic[2018 Feb]; Mixed (Chlorogonium capillatum NIES-3374 as food)  
Culture condition
(Preculture condition)
Medium:  Helio  
Temperature:  20 C
Light intensity:  4-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  21 D  
Gene information gp40-1 ( AB368718 ) , gp40-2 ( AB368719 ) , mRNA for sedolisin-like peptidase ( AB276124 )  
Cell size (min - max)  
Other strain no. Other strain no. : HSA  
Remarks Axenic 
Azuma, T., Pánek, T., Tice, A. K., Kayama, M., Kobayashi, M., Miyashita, H., Suzaki, T., Yabuki, A., Brown, M. W., Kamikawa, R. 2022 An enigmatic stramenopile sheds light on early evolution in Ochrophyta plastid organellogenesis. Mol. Biol. Evol., 39, msac065 (article ID).
Keywords: Actinophryidae; plastid evolution; aminoacyl-tRNA synthase; gene transfer; phylogenomics; organellar DNA
Strain(s): 24973374 
PubMed: 35348760
DOI: 10.1093/molbev/msac065

Kakuta, S., Suzaki, T. 2007 Ca2+- and glycoconjugates-dependent prey capture in the heliozoan Actinophrys sol. Eur. J. Protistol., 44, 163-167.
Keywords: calium ion influx; Con A; exocytosis; extrusome; glycoprotein; secretory granule
Strain(s): 2497 
PubMed: 18178392
DOI: 10.1016/j.ejop.2007.11.001

Arikawa, M., Saito, A., Omura, G., Khan, S. M. M. K., Suetomo, Y., Kakuta, S., Suzaki, T. 2006 Ca2+-dependent in vivo contractility of a precipitate isolated from an extract of the heliozoan Actinophrys sol. Cell Motil. Cytoskeleton, 63, 57-65.
Keywords: contraction; precipitate; heliozoan; protozoa
Strain(s): 2497 
PubMed: 16362955
DOI: 10.1002/cm.20106

Arikawa, M., Saito, A., Omura, G., Khan, S. M. M. K., Suetomo, Y., Kakuta, S., Suzaki, T. 2005 Ca2+-dependent nuclear contraction of heliozoan Actinophrys sol. Cell Calcium, 38, 447-455.
Strain(s): 2497 
PubMed: 16099499
DOI: 10.1016/j.ceca.2005.06.034

Arikawa, M., Saito, A., Omura, G., Khan, S. M. M. K., Kinoshita, E., Suzaki, T. 2002 Ca2+-dependent cytoplasmic contractility of the heliozoan Actinophrys sol. Eur. J. Protistol., 38, 365-372.
Keywords: Cell model; Permeabilization; Contractile tubules; Heliozoa; Protozoa
Strain(s): 2497 
DOI: 10.1078/0932-4739-00879

Sakaguchi, M., Murakami, H., Suzaki, T. 2001 Involvement of a 40-kDa glycoprotein in food recognition, prey capture, and induction of phagocytosis in the protozoon Actinophrys sol. Protist, 152, 33-41.
Strain(s): 24973374 
PubMed: 11401035
DOI: 10.1078/1434-4610-00041

Kinoshita, E., Suzaki, T., Shigenaka, Y. 2001 The ultrastructure of contractile tubules in the heliozoan Actinophrys sol and their possible involvement in rapid axopodial contraction. J. Eucaryot. Microbiol., 48, 519-526.
Keywords: Axopodium; Ca2+-contraction; colchicine; electron microscopy
Strain(s): 2497 
PubMed: 11596916
DOI: 10.1111/j.1550-7408.2001.tb00187.x

Sakaguchi, M., Suzuki, T. 1999 Monoxenic culture of the heliozoon Actinophrys sol. Eur. J. Protistol., 35, 1-10.
Keywords: Actinophrys sol; Antibiotic; Heliozoa; Monoxenic Culture
Strain(s): 24973374 
DOI: 10.1016/S0932-4739(99)80050-9

Sakaguchi, M., Hausmann, K., Suzaki, T. 1998 Food capture and adhesion by the heliozoon Actinophrys sol. Protoplasma, 203, 130-137.
Keywords: Actinophrys sol; Exocytosis; Extrusomes; Feeding behavior; Heliozoa; Prey adhesion
Strain(s): 2497 
DOI: 10.1007/BF01279469

Sakaguchi, M., Suzaki, T., Shigenaka, Y. 1997 Statistical analysis of spatial patterns of the heliozoan Actinophrys sol. Protoplasma, 196, 117-122.
Keywords: Heliozoa; Spatial point pattern; Cell distribution; Interaction potential; Likelihood analysis
Strain(s): 2497 
DOI: 10.1007/BF01281065

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