Strain number NIES-204  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Planktothrix agardhii (Gomont) Anagnostidis & Komárek  
Synonym Oscillatoria agardhii Gomont 1892; Oscillatoria agardhii f. gomontii Elenkin 1949; Oscillatoria agardhii f. aequicrassa Elenkin 1949; Oscillatoria agardhii f. moebiusii Elenkin 1949; Oscillatoria agardhii f. lemmermannii Elenkin 1949; Oscillatoria agardhii f. wislouchii Elenkin 1949  
Former name  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Kasumigaura, Ibaraki, Japan (1983-08-24)  
Latitude / Longitude 36.081204 / 140.214193 
Habitat (Isolation source) Freshwater (Lake water)  
History < Suda, Shoichiro  
Isolator (Date of isolation) Suda, Shoichiro (1983-08-30)  
Identified by Suda, Shoichiro  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  25 C
Light intensity:  20-30 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Whole-genome ( AP017991-AP017995 ) , 16S rRNA ( AB045954 AB074507 ) , OAA ( AB453976 ) , rpoD1 ( AB074817 ) , gyrB ( AB074767 ) , rpoC1 ( AB074790 )  
Cell size (min - max) 3 - 4 μm  
Organization Filamentous 
Characteristics Cyanobacterial water bloom (aoko) ; Type strain (Suda, S. et al. 2002) ; Genome decoded strain (Shimura et al. 2021)  
Other strain no. Other collection strain no. : IAM M-244; CCAP 1460/5; PCC 10704
Other strain no. : K-O-A  
Remarks Cryopreserved; Axenic 
Giordano, M., Goodman, C. A., Huang, F., Raven, J. A., Ruan, Z. 2022 A mechanistic study of the influence of nitrogen and energy availability on the NH4+ sensitivity of nitrogen assimilation in Synechococcus. J. Exp. Bot., 73, 5596-5611.
Keywords: Ammonium; cyanobacteria; glutamine synthetase; limitation; nitrate reductase; nitrite reductase; NtcA regulation; N metabolism
Strain(s): 25502042678439709812107213421352481254937093754375637574074 
PubMed: 35595516
DOI: 10.1093/jxb/erac219

Shimura, Y., Fujisawa, T., Hirose, Y., Misawa, N., Kanesaki, Y., Nakamura, Y., Kawachi, M. 2021 Complete sequence and structure of the genome of the harmful algal bloom-forming cyanobacterium Planktothrix agardhii NIES-204T and detailed analysis of secondary metabolite gene clusters. Harmful Algae, 101, 101942 (article ID).
Keywords: 5S ribosomal RNA gene; Aeruginosin; Horizontal gene transfer; Microcystis; Prenyltransferase; Oscillatorin
Strain(s): 204 
PubMed: 33526179
DOI: 10.1016/j.hal.2020.101942

Carbone, D. A., Pellone, P., Lubritto, C., Ciniglia, C. 2021 Evaluation of microalgae antiviral activity and their bioactive compounds. Antibiotics-Basel, 10, 746 (article ID).
Keywords: microalgae; virus; antiviral compounds; SARS-CoV-2; microalgae antiviral activity; coronavirus disease
Strain(s): 204 
PubMed: 34202941
DOI: 10.3390/antibiotics10060746

Monteiro, P. R., do Amaral, S. C., Siqueira, A. S., Xavier, L. P., Santos, A. V. 2021 Anabaenopeptins: What We Know So Far. Toxins, 13, 522 (article ID).
Keywords: cyanobacteria; peptide; NRPS; anabaenopeptin
Strain(s): 81204595 
PubMed: 34437393
DOI: 10.3390/toxins13080522

Rahman, M. M., Rahaman, M. S., Islam, M. R., Hossain, M. E., Mithi, FM., Ahmed, M., Saldías, M., Akkol, E. K., Sobarzo-Sánchez, E. 2021 Multifunctional therapeutic potential of phytocomplexes and natural extracts for antimicrobial properties. Antibiotics-Basel, 10, 1076 (article ID).
Keywords: natural products; phytocomplexes; antimicrobial activity; alkaloids; tannins; terpenoids
Strain(s): 204 
PubMed: 34572660
DOI: 10.3390/antibiotics10091076

Chen, M., Tian, L.-L., Ren, C.-Y., Xu, C.-Y., Wang, Y.-Y., Li, L. 2019 Extracellular polysaccharide synthesis in a bloom-forming strain of Microcystis aeruginosa: implications for colonization and buoyancy. Sci Rep, 9, 1251 (article ID).
Strain(s): 87889820429884324812549 
PubMed: 30718739
DOI: 10.1038/s41598-018-37398-6

Selivanova, E. A., Poshvina, D. V., Khlopko, Yu. A., Gogoleva, N. E., Plotnikov, A. O. 2018 Diversity of prokaryotes in planktonic communities of saline sol-iletsk lakes (Orenburg Oblast, Russia). Microbiology, 87, 569-582.
Keywords: saline lakes; biodiversity; prokaryotes; halophilic bacteria; archaea; planktonic communities; high-throughput sequencing; Illumina; 16S rRNA gene
Strain(s): 2041267 
DOI: 10.1134/S0026261718040161

Kaya, K., Sano, T. 2016 Chapter 18. Cyanobacterial retinoids. In Handbook of cyanobacterial monitoring and cyanotoxin analysis, Eds. byMeriluoto, J., Spoof, L., Codd, G. A., John Wiley & Sons, Ltd. pp. 173-178.
Strain(s): 364445462045126101222 
DOI: 10.1002/9781119068761.ch18

Oyama, Y., Matsushita, B., Fukushima, T., Chen, J., Nagai, T., Imai, A. 2010 Testing the spectral decomposition algorithm (SDA) for different phytoplankton species by a simulation based on tank experiments. Int. J. Remote Sens., 31, 1605-1623.
Strain(s): 4475204803 
DOI: 10.1080/01431160903475365

Sato, T. & Hori, K. 2009 Cloning, expression, and characterization of a novel anti-HIV lectin from the cultured cyanobacterium, Oscillatoria agardhii. Fish. Sci., 75, 743-753.
Keywords: anti-HIV; high-mannose N-glycans; Oscillatoria agardhii; lectin; recombinant lectin
Strain(s): 204 
DOI: 10.1007/s12562-009-0074-4

Tomioka, N., Nagai, T., Kawasaki, T., Imai, A., Kohata, K. 2008 Quantification of Microcystis in a eutrophic lake by simple DNA extraction and SYBR green real-time PCR. Microbes Environ., 23, 306-312.
Keywords: Microcystis; quantification; SYBR Green I; DNA extraction; eutrophic lake
Strain(s): 9899204604611843946961 
DOI: 10.1264/jsme2.ME08515

Shinohara, A., Imai, A., Komatsu, K., Matsushige, K., Nara, F. 2008 Application of HPLC-PAD to highly sensitive analysis of dissolved carbohydrates and theit composition in lake water and extracellular organic matter derived from algae. J. Jpn. Soc. Wat. Environ., 31, 447-454 (in Japanese with English summary).
Keywords: carbohydrates; DOM; HPLC-PAD; lake water; algal culture
Strain(s): 4474204 
DOI: 10.2965/jswe.31.447

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2007 Growth characteristics and growth modeling of Microcystis aeruginosa and Planktothrix agardhii under iron limitation. Limnology, 8, 261-270.
Keywords: iron; growth rate; uptake rate; model simulation; cyanobacteria
Strain(s): 44204 
DOI: 10.1007/s10201-007-0217-1

Sato, Y., Okuyama, S., Hori, K. 2007 Primary structure and carbohydrate binding specificity of a potent anti-HIV lectin isolated from the filamentous cyanobacterium Oscillatoria agardhii. J. Biol. Chem., 282, 11021-11029.
Strain(s): 204 
PubMed: 17314091
DOI: 10.1074/jbc.M701252200

Jang, M.-H., Ha, K., Jung, J.-M., Lee, Y.-J., Takamura, N. 2006 Increased microcystin production of Microcystis aeruginosa by indirect exposure of nontoxic cyanobacteria: potential role in the development Microcystis bloom. Bull. Environ. Contam. Toxicol., 76, 957-962.
Strain(s): 7488204 

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2006 Effect of iron complexation with dissolved organic matter on the growth of cyanobacteria in a eutrophic lake. Aquat. Microb. Ecol., 44, 231-239.
Keywords: dissolved organic matter; DOM; cyanobacteria; iron; organic complexation
Strain(s): 44204 
DOI: 10.3354/ame044231

Hosoya, R., Hamana, K., Isobe, M., Yokota, A. 2005 Polyamine distribution profiles within Cyanobacteria. Microbiol. Cult. Coll., 21, 3-8.
Keywords: cyanobacteria; homospermidine; polyamine; spermidine
Strain(s): 19212228303437414445507378798081204208510512515592593610843846847209320962098210021022107210821182119212321242125212621282129213121323259327532763277 

Nagai, T., Imai, A., Matsushige, K., Fukushima, T. 2005 Limiting nutrients on the growth of bloom-forming cyanobacteria with special focus on iron speciation. Verh. Internat. Verein. Limnol., 29, 949-952.
Keywords: limiting nutrient; algal growth potential test; speciation; iron
Strain(s): 44204 
DOI: 10.1080/03680770.2005.11902823

Sato, M., Murata, Y., Mizusawa, M., Iwahashi, H., Oka, S.-I. 2004 A simple and rapid dual-fluorescence viability assay for microalgae. Microbiol. Cult. Coll., 20, 53-59.
Keywords: microalgae; SYTOX Green; viability assay; autofluorescence
Strain(s): 32204 

Hirabayashi, R. & Imamura, N. 2003 Action mechanism of a selective anti-cyanobacterial compound, argimicin A. J. Antibiotics, 56, 154-159.
Strain(s): 414581102204230298592 
PubMed: 12715875
DOI: 10.7164/antibiotics.56.154

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Suda, S., Watanabe, M. M., Otsuka, S., Mahakahant, A., Yongmanitchai, W., Nopartnaraporn, N., Liu, Y., Day, J. G. 2002 Taxonomic revision of water-bloom-forming species of oscillatorioid cyanobacteria. Int. J. Syst. Evol. Microbiol., 52, 1577-1595.
Keywords: cyanobacteria; water bloom; oscillatorioid; nomenclature; taxonomy
Strain(s): 204205207594595596610844845846847905911912913915916917918 
PubMed: 12361260
DOI: 10.1099/00207713-52-5-1577

Yuki, Y., Yoshida, T., Hiroishi, S. 2002 Molecular detection of the toxic cyanobacteria [Yûdoku aoko no bunshi-shikibetsu to yosatsu he-no ôyô]. In Series of Fisheries Science Vol. 143, Prevention and Extermination Strategies of Harmful Algal Blooms [Suisan-gaku shirîzu 134, Yûgai-yûdoku sôrui burûmu no yobô to kujo] , Eds. by Hiroishi, S., Imai, I. & Ishimaru, T., Koseisha-Koseikaku, Tokyo, pp. 43-53 (in Japanese).
Strain(s): 738187888990919899100101102104105106108109110111112204230298299604 

Choi, K., Imai, A., Kim, B., Matsushige, K. 2001 Properties of dissolved organic carbon (DOC) released by three species of blue-green algae. Korean J. Limnol., 34, 20-29.
Keywords: extracellular organic carbon; fractionation; blue-green algae; growth phases
Strain(s): 4474204 

Sato, Y., Murakami, M., Miyazawa, K., Hori, K. 2000 Purification and characterization of a novel lectin from a freshwater cyanobacterium, Oscillatoria agardhii. Comp. Biochem. Physiol., Part B 125, 169-177.
Keywords: agglutinins; blue-green algae; cyanobacteria; carbohydrate-binding specificity; isolation; lectin; eucheuma; Myxococcus xanthus; Oscillatoria agardhii; N-terminal sequence
Strain(s): 204 
PubMed: 10817903
DOI: 10.1016/S0305-0491(99)00164-9

Itou, Y., Ishida, K., Shin, H. J., Murakami, M. 1999 Oscillapeptins A to F, serine protease inhibitors from the three strains of Oscillatoria agardhii. Tetrahedron, 55, 6871-6882.
Strain(s): 204205596 
DOI: 10.1016/S0040-4020(99)00341-5

Neilan, B. A., Dittmann, E., Rouhiainen, L., Bass, R. A., Schaub, V., Sivonen, K., Börner, T. 1999 Nonribosomal peptide synthesis and toxigenicity of Cyanobacteria. J. Bacteriol., 181, 4089-4097.
Strain(s): 19248199102107204 
PubMed: 10383979
DOI: 10.1128/JB.181.13.4089-4097.1999

Li, R., Yokota, A., Sugiyama, J., Watanabe, M., Hiroki, M., Watanabe, M. M. 1998 Chemotaxonomy of planktonic cyanobacteria based on non-polar and 3-hydroxy fatty acid composition. Phycol. Res., 46, 21-28.
Keywords: 3-hydroxy fatty acids; chemotaxonomy; fatty acid; non-polar fatty acids; planktonic cyanobacteria
Strain(s): 2740447678798081878998102104111112204205207298604810821825826 
DOI: 10.1111/j.1440-1835.1998.tb00093.x

Murakami, M., Shin, H. J., Matsuda, H., Ishida, K., Yamaguchi, K. 1997 A cyclic peptide, anabaenopeptin B, from the cyanobacterium Oscillatoria agahdhii. Phytochemistry, 44, 449-452.
Keywords: Oscillatoria agardhii; cyanobacterium; cyclic peptide; anabaenopeptin B
Strain(s): 204 
DOI: 10.1016/S0031-9422(96)00437-2

Shin, H. J., Matsuda, H., Murakami, M., Yamaguchi, K. 1997 Anabaenopeptins E and F, two new cyclic peptides from the cyanobacterium Oscillatoria agardhii (NIES-204). J. Nat. Prod., 60, 139-141.
Strain(s): 204 
DOI: 10.1021/np960597p

Shin, H. J., Matsuda, H., Murakami, M., Yamaguchi, K. 1996 Agardhipeptins A and B, two new cyclic hepta- and octapeptide, from the cyanobacterium Oscillatoria agardhii (NIES-204). Tetrahedron, 52, 13129-13136.
Strain(s): 204 
DOI: 10.1016/0040-4020(96)00775-2

Shin, H. J., Murakami, M., Matsuda, H., Yamaguchi, K. 1996 Microviridins D-F, serine protease inhibitors from the cyanobacterium Oscillatoria agardhii (NIES-204). Tetrahedron, 52, 8159-8168.
Strain(s): 204 
DOI: 10.1016/0040-4020(96)00377-8

Neilan, B. A., Jacobs, D., Goodman, A. E. 1995 Genetic diversity and phylogeny of toxic cyanobacteria determined by DNA polymorphisms within the phycocyanin locus. Appl. Environ. Microbiol., 61, 3875-3883.
Strain(s): 244041447376788081878998102107111112204207298 
PubMed: 8526499

Shin, H. J., Murakami, M., Matsuda, H., Ishida, K., Yamaguchi, K. 1995 Oscillapeptin, an elastase and chymotrypsin inhibitor from the cyanobacterium Oscillatoria agardhii (NIES-204). Tetrahedron Letters, 36, 5235-5238.
Strain(s): 204 
DOI: 10.1016/0040-4039(95)00980-Q

Watanabe, Y., Ohmura, N., Saiki, H. 1992 Microbial CO2 fixation. 2. Isolation and determination of cultural characteristics of Chlorella strains which function under CO2 enriched atmosphere. CRIEPI Kenkyû-Hôkoku, U92014, 1-21 (in Japanese with English summary).
Keywords: climate change; CO2; CO2 fixation; microalgae; Chlorella
Strain(s): 27459294122158204226227 

Watanabe, M. M., Kasai, F., Hiwatari, T., Suda, S., Nei, T. 1984 Cryopreservation of microalgae in liquid nitrogen - Survival of algal cultures after freezing. Jpn. J. Freez. Dry., 30, 23-26 (in Japanese with English title).
Strain(s): 1819212426293139404144454650737475767780818788899091939598991001011201221311351371441512042072132572633794152219223522392579 

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