Strain Data

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Strain number		NIES-15
Phylum		Heterokontophyta
Class		Olisthodiscophyceae
Scientific name		Olisthodiscus tomasii Barcytė, Eikrem & M.Eliáš
Synonym
Former name		Olisthodiscus luteus N.Carter
Common name
Locality (Date of collection)		Seto Inland Sea, Tamano, Okayama, Japan
Latitude / Longitude
Habitat (Isolation source)		Marine (Coastal soil)
History		< Inouye, Isao
Isolator (Date of isolation)		Inouye, Isao
Identified by		Inouye, Isao
State of strain		Subculture; Unialgal; Clonal; Axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 6 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 2 M
Gene information		18S rRNA , 18S-ITS1-5.8S-ITS2 , 28S rRNA , GapC1 , GapC2 , rbcL
Cell size (min - max)		- 19 μm
Organization		Unicellular; Flagellate
Characteristics		Red tide ; Authentic strain, Holotype (Barcytė et al. 2021)
Other strain no.		Other strain no. : Olisth
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"; Axenic
Movie		https://www.youtube.com/watch?v=gV_ikdEymk4&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=19

Reference
Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID). Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778 DOI: 10.1016/j.algal.2021.102191 Barcytė, D., Eikrem, W., Engesmo, A., Seoane, S., Wohlmann, J., Horák, A., Yurchenko, T., Eliáš, M. 2021 Olisthodiscus represents a new class of Ochrophyta. J. Phycol., 57, 1094-1118. Strain(s): 15 PubMed: 33655496 DOI: 10.1111/jpy.13155 Engesmo, A., Strand, D., Gran-Stadniczeñko, S., Edvardsen, B., Medlin, LK., Eikrem, W. 2018 Development of a qPCR assay to detect and quantify ichthyotoxic flagellates along the Norwegian coast, and the first Norwegian record of Fibrocapsa japonica (Raphidophyceae). Harmful Algae, 75, 105-117. Keywords: Karenia mikimotoi; Karlodinium veneficum; Heterosigma akashiwo; Molecular monitoring; rDNA; 454 high throughput environmental; sequencing; SEM Strain(s): 15 PubMed: 29778220 DOI: 10.1016/j.hal.2018.04.007 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 Takishita, K., Yamaguchi, H., Maruyama, T., Inagaki, Y. 2009 A hypothesis for the evolution of nuclear-encoded, plastid-targeted glyceraldehyde-3 phosphate dehydrogenase genes in "chromalveolate" members. PLoS One, 4, e4737 (article ID). Strain(s): 15, 275, 706, 716 PubMed: 19270733 DOI: 10.1371/journal.pone.0004737 Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140. Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444 PubMed: 18497488 DOI: 10.2323/jgam.54.135 Iida, K., Takishita, K., Ohshima, K., Inagaki, Y. 2007 Assessing the monophyly of chlorophyll-c containing plastids by multi-gene phylogenies under the unlinked model conditions. Mol. Phylogenet. Evol., 45(1), 227-238. Keywords: chromist; chromalveolate; secondary endosymbiosis; model misspecification; separate model; concatenate model Strain(s): 1, 15, 548, 670, 716 PubMed: 17591448 DOI: 10.1016/j.ympev.2007.05.003 Alamsjah, M. A., Hirao, S., Ishibashi, F., Fujita , Y. 2005 Isolation and structure determination of algicidal compounds from Ulva fasciata. Biosci. Biotechnol. Biochem., 69, 2186-2192. Keywords: algicidal compounds; Ulva fasciata; hexadeca-4,7,10,13-tetraenoic acid; octadeca-6,9,12,15-tetraenoic acid; α-linolenic acid Strain(s): 15, 462 PubMed: 16306701 DOI: 10.1271/bbb.69.2186 Kuroda, A., Nakashima, T., Yamaguchi, K., Oda, T. 2005 Isolation and characterization of light-dependent hemolytic cytotoxin fromharmful red tide phytoplankton Chattonella marina. Comp. Biochem. Physiol. C-Pharmacol. Toxicol. Endocrinol., 141, 297-305. Keywords: Red tide; Phytoplankton; Raphidophycean flagellate; Chattonella marina; Hemolysin; Photosensitizer; Pheophorbide a; Cytotoxin; Photodynamic therapy Strain(s): 6, 15 PubMed: 16098818 DOI: 10.1016/j.cca.2005.07.009 Marshall, J. A., Nichols, P. D., Hallegraeff, G. M. 2002 Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol., 14, 255-265. Keywords: Chattonella antiqua; Chattonella marina; Chattonella subsalsa; chemotaxonomy; fatty acids; Fibrocapsa japonica; Heterosigma akashiwo; Olisthodiscus luteus; phycotoxins; raphydophytes; sterols Strain(s): 15, 118 Connell, L. 2002 Rapid identification of marine algae (Raphidophyceae) using three-primer peR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia, 41, 15-21. Strain(s): 1, 15 DOI: 10.2216/i0031-8884-41-1-15.1 Oda, T., Sato, Y., Kim, D., Muramatsu, T., Matsuyama, Y., Honjo, T. 2001 Hemolytic activity of Heterocapsa circularisquama (Dinophyceae) and its possible involvement in shellfish toxicity. J. Phycol., 37, 509-516. Keywords: Heterocapsa circularisquama; hemolysis; shellfish toxicity; harmful phytoplankton Strain(s): 15 DOI: 10.1046/j.1529-8817.2001.037004509.x Kim, D., Okamoto, T., Oda, T., Tachibana, K., Lee, K. S., Ishimatsu, A., Matsuyama, Y., Honjo, T., Muramatsu, T. 2001 Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina (Raphidophyceae): immunological approach using antiserum against cell surface structures of the flagellate. Mar. Biol., 139, 625-632. Strain(s): 6, 15 DOI: 10.1007/s002270100614 Kim, D., Nakamura, A., Okamoto, T., Komatsu, N., Oda, T., Ishimatsu, A., Muramatsu, T. 1999 Toxic potential of the raphidophyte Olisthodiscus luteus: mediation by reactive oxygen species. J. Plankton Res., 21, 1017-1027. Strain(s): 15 DOI: 10.1093/plankt/21.6.1017 Oda, T., Nakamura, A., Shikayama, M., Kawano,I., Ishimatsu, A., Muramatsu, T. 1997 Generation of reactive oxygen species by Raphidophycean phytoplankton. Biosci. Biotechnol. Biochem., 61, 1658-1662. Keywords: red tide planlton; Raphidophyceae; Chattonella marina; reactive oxygen species; toxicity Strain(s): 6, 15 PubMed: 9362113 DOI: 10.1271/bbb.61.1658 Yasumoto, T. 1992 Toxin of the marine micro-algae [Kaiyô bisaisôrui no Tokishin]. BIO Medica, 7, 57-62 (in Japanese). Strain(s): 1, 6, 12, 15 Uye, S. & Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107. Strain(s): 12, 15, 221, 235, 251 Nagai, H., Satake, M., Murata, M., Yasumoto, T. 1990 Screening of marine phytoplankton for antifungal substances. In Toxic Marine Phyto-plankton, Eds. by Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M., Elsevier Science Pub., New York, pp. 385-390. Strain(s): 1, 6, 12, 15 Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227. Strain(s): 5, 15, 161 DOI: 10.2216/i0031-8884-28-2-222.1 Hara, Y., Inouye, I., Chihara, M. 1985 Morphology and ultrastructure of Olisthodiscus luteus (Raphidophyceae) with special reference to the taxonomy. Bot. Mag. Tokyo, 98, 251-262. Keywords: Heterosigma; Olisthodiscus luteus; Raphidophyceae; red tide flagellate; taxonomy of Olisthodiscus; ultrastructure of Olisthodiscus Strain(s): 15 DOI: 10.1007/BF02488775

Reference

Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID).
Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles
Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778
DOI: 10.1016/j.algal.2021.102191

Barcytė, D., Eikrem, W., Engesmo, A., Seoane, S., Wohlmann, J., Horák, A., Yurchenko, T., Eliáš, M. 2021 Olisthodiscus represents a new class of Ochrophyta. J. Phycol., 57, 1094-1118.
Strain(s): 15
PubMed: 33655496
DOI: 10.1111/jpy.13155

Engesmo, A., Strand, D., Gran-Stadniczeñko, S., Edvardsen, B., Medlin, LK., Eikrem, W. 2018 Development of a qPCR assay to detect and quantify ichthyotoxic flagellates along the Norwegian coast, and the first Norwegian record of Fibrocapsa japonica (Raphidophyceae). Harmful Algae, 75, 105-117.
Keywords: Karenia mikimotoi; Karlodinium veneficum; Heterosigma akashiwo; Molecular monitoring; rDNA; 454 high throughput environmental; sequencing; SEM
Strain(s): 15
PubMed: 29778220
DOI: 10.1016/j.hal.2018.04.007

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691

Takishita, K., Yamaguchi, H., Maruyama, T., Inagaki, Y. 2009 A hypothesis for the evolution of nuclear-encoded, plastid-targeted glyceraldehyde-3 phosphate dehydrogenase genes in "chromalveolate" members. PLoS One, 4, e4737 (article ID).
Strain(s): 15, 275, 706, 716
PubMed: 19270733
DOI: 10.1371/journal.pone.0004737

Hamana, K. 2008 Cellular polyamines of phototrophs and beterotrophs belonging to the lower eukaryotic phyla Cercozoa, Euglenozoa, Heterokonta and Metamonada. J. Gen. Appl. Microbiol., 54, 135-140.
Keywords: cyanobacteria; homospermidine; polyamine; spermine; thermospermine
Strain(s): 9, 15, 71, 86, 118, 253, 323, 330, 333, 548, 603, 622, 1002, 1003, 1011, 1044, 1303, 1380, 1440, 1443, 1444
PubMed: 18497488
DOI: 10.2323/jgam.54.135

Iida, K., Takishita, K., Ohshima, K., Inagaki, Y. 2007 Assessing the monophyly of chlorophyll-c containing plastids by multi-gene phylogenies under the unlinked model conditions. Mol. Phylogenet. Evol., 45(1), 227-238.
Keywords: chromist; chromalveolate; secondary endosymbiosis; model misspecification; separate model; concatenate model
Strain(s): 1, 15, 548, 670, 716
PubMed: 17591448
DOI: 10.1016/j.ympev.2007.05.003

Alamsjah, M. A., Hirao, S., Ishibashi, F., Fujita , Y. 2005 Isolation and structure determination of algicidal compounds from Ulva fasciata. Biosci. Biotechnol. Biochem., 69, 2186-2192.
Keywords: algicidal compounds; Ulva fasciata; hexadeca-4,7,10,13-tetraenoic acid; octadeca-6,9,12,15-tetraenoic acid; α-linolenic acid
Strain(s): 15, 462
PubMed: 16306701
DOI: 10.1271/bbb.69.2186

Kuroda, A., Nakashima, T., Yamaguchi, K., Oda, T. 2005 Isolation and characterization of light-dependent hemolytic cytotoxin fromharmful red tide phytoplankton Chattonella marina. Comp. Biochem. Physiol. C-Pharmacol. Toxicol. Endocrinol., 141, 297-305.
Keywords: Red tide; Phytoplankton; Raphidophycean flagellate; Chattonella marina; Hemolysin; Photosensitizer; Pheophorbide a; Cytotoxin; Photodynamic therapy
Strain(s): 6, 15
PubMed: 16098818
DOI: 10.1016/j.cca.2005.07.009

Marshall, J. A., Nichols, P. D., Hallegraeff, G. M. 2002 Chemotaxonomic survey of sterols and fatty acids in six marine raphidophyte algae. J. Appl. Phycol., 14, 255-265.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella subsalsa; chemotaxonomy; fatty acids; Fibrocapsa japonica; Heterosigma akashiwo; Olisthodiscus luteus; phycotoxins; raphydophytes; sterols
Strain(s): 15, 118

Connell, L. 2002 Rapid identification of marine algae (Raphidophyceae) using three-primer peR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia, 41, 15-21.
Strain(s): 1, 15
DOI: 10.2216/i0031-8884-41-1-15.1

Oda, T., Sato, Y., Kim, D., Muramatsu, T., Matsuyama, Y., Honjo, T. 2001 Hemolytic activity of Heterocapsa circularisquama (Dinophyceae) and its possible involvement in shellfish toxicity. J. Phycol., 37, 509-516.
Keywords: Heterocapsa circularisquama; hemolysis; shellfish toxicity; harmful phytoplankton
Strain(s): 15
DOI: 10.1046/j.1529-8817.2001.037004509.x

Kim, D., Okamoto, T., Oda, T., Tachibana, K., Lee, K. S., Ishimatsu, A., Matsuyama, Y., Honjo, T., Muramatsu, T. 2001 Possible involvement of the glycocalyx in the ichthyotoxicity of Chattonella marina (Raphidophyceae): immunological approach using antiserum against cell surface structures of the flagellate. Mar. Biol., 139, 625-632.
Strain(s): 6, 15
DOI: 10.1007/s002270100614

Kim, D., Nakamura, A., Okamoto, T., Komatsu, N., Oda, T., Ishimatsu, A., Muramatsu, T. 1999 Toxic potential of the raphidophyte Olisthodiscus luteus: mediation by reactive oxygen species. J. Plankton Res., 21, 1017-1027.
Strain(s): 15
DOI: 10.1093/plankt/21.6.1017

Oda, T., Nakamura, A., Shikayama, M., Kawano,I., Ishimatsu, A., Muramatsu, T. 1997 Generation of reactive oxygen species by Raphidophycean phytoplankton. Biosci. Biotechnol. Biochem., 61, 1658-1662.
Keywords: red tide planlton; Raphidophyceae; Chattonella marina; reactive oxygen species; toxicity
Strain(s): 6, 15
PubMed: 9362113
DOI: 10.1271/bbb.61.1658

Yasumoto, T. 1992 Toxin of the marine micro-algae [Kaiyô bisaisôrui no Tokishin]. BIO Medica, 7, 57-62 (in Japanese).
Strain(s): 1, 6, 12, 15

Uye, S. & Takamatsu, K. 1990 Feeding inter-actions between planktonic copepods and red-tide flagellates from Japanese coastal waters. Mar. Ecol. Prog. Ser., 59, 97-107.
Strain(s): 12, 15, 221, 235, 251

Nagai, H., Satake, M., Murata, M., Yasumoto, T. 1990 Screening of marine phytoplankton for antifungal substances. In Toxic Marine Phyto-plankton, Eds. by Graneli, E., Sundstrom, B., Edler, L. & Anderson, D. M., Elsevier Science Pub., New York, pp. 385-390.
Strain(s): 1, 6, 12, 15

Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227.
Strain(s): 5, 15, 161
DOI: 10.2216/i0031-8884-28-2-222.1

Hara, Y., Inouye, I., Chihara, M. 1985 Morphology and ultrastructure of Olisthodiscus luteus (Raphidophyceae) with special reference to the taxonomy. Bot. Mag. Tokyo, 98, 251-262.
Keywords: Heterosigma; Olisthodiscus luteus; Raphidophyceae; red tide flagellate; taxonomy of Olisthodiscus; ultrastructure of Olisthodiscus
Strain(s): 15
DOI: 10.1007/BF02488775

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