Strain number NIES-2293  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Hamakko caudatus Nakada  
Former name  
Common name Green alga  
Locality (Date of collection) Kohoku-ku, Yokohama, Kanagawa, Japan (2003-05-12)  
Latitude / Longitude 35.503258736 / 139.596789656 
Habitat (Isolation source) Freshwater (Soil)  
History < Nakada, Takashi  
Isolator (Date of isolation) Nakada, Takashi (2004-05-11)  
Identified by Nakada, Takashi  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  AF-6MG  
Temperature:  20 C
Light intensity:  10-17 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB451188 ) , ITS1-5.8S-ITS2 ( LC095630 ) , 26S rRNA ( LC066337 ) , atpB ( LC066347 ) , psaB ( AB451212 ) , rbcL ( AB451193 )  
Cell size (min - max)  
Characteristics Authentic strain (Nakada, T. et al. 2009)  
Other strain no. Other strain no. : KzCl-4-1  
Remarks Axenic 
Harmon, J., Mikami, H., Kanno, H., Ito, T., Goda, K. 2020 Accurate classification of microalgae by intelligent frequency-division-multiplexed fluorescence imaging flow cytometry. OSA Continuum, 3, 430-440.
Strain(s): 229336404141 
DOI: 10.1364/OSAC.387523

Nitta, N. et al. 2020 Raman image-activated cell sorting. Nat. Commun., 11, 3452 (article ID).
Strain(s): 48229324633640 
PubMed: 32651381
DOI: 10.1038/s41467-020-17285-3

Temraleeva, A. D., Moskalenko, S. V., Bachura, Y. M. 2017 Morphology, ecology, and 18S rDNA phylogeny of the green microalgal order Protosiphonales (Chlorophyceae, Chlorophyta). Microbiology, 86, 159-169.
Keywords: green microalgae; morphology; ecology; molecular phylogeny; polyphasic approach
Strain(s): 2293 
DOI: 10.1134/S0026261717020205

Munakata, H., Nakada, T., Nakahigashi, K., Nozaki, H., Tomita, M. 2016 Phylogenetic position and molecular chronology of a colonial green flagellate, Stephanosphaera pluvialis (Volvocales, Chlorophyceae), among unicellular algae. J. Eucaryot. Microbiol., 63, 340-348.
Keywords: Balticola; coloniality; divergence time; evolution; molecular phylogeny
Strain(s): 12314442142843243944656756957165686188796810482293256441244125 
PubMed: 26595722
DOI: 10.1111/jeu.12283

Nakada, T. & Nozaki, H. 2009 Taxonomic study of two new genera of fusiform green flagellates, Tabris gen. nov. and Hamakko gen. nov. (Volvocales, Chlorophyceae). J. Phycol., 45, 482-492.
Keywords: Chlorogonium; Hamakko gen. nov.; molecular phylogeny; Tabris gen. nov.; taxonomy; ultrastructure; Volvocales
Strain(s): 122637748752760968135813591849185018692293229422954125 
PubMed: 27033826
DOI: 10.1111/j.1529-8817.2009.00652.x

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