Strain Data

Strain number		NIES-2285
Phylum		Streptophyta
Class		Charophyceae
Scientific name		Klebsormidium nitens (Kützing) Lokhorst
Synonym		Ulothrix nitens Kützing; Hormidium nitens (Kützing) Rabenhorst 1863; Chlorhormidium flaccidum var. nitens (Kützing) Farooqui 1969; Chlorhormidium nitens (Meneghini) Komaromy 1976
Former name		Formerly identified as Klebsormidium flaccidum (Kützing) Silva, Mattox & Blackwell[2016 Jun]; Hormidium barlowi
Common name
Locality (Date of collection)		Barlow, Madison, Wisconsin, USA
Latitude / Longitude		43.073308 /
Habitat (Isolation source)		Freshwater
History		< IAM (2007) < BIU (UTEX; 1965);
Isolator (Date of isolation)		Pringsheim, E. G.
Identified by		Handa, Shinji (Reidentify)
State of strain		Subculture; Unialgal; Clonal; Axenic[2020 Nov]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 5-9 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 6 M
Gene information		Whole-genome ( BANV00000000 ) , Mitochondria DNA ( DF238763 ) , Plastid DNA ( DF238762 )
Cell size (min - max)		5 - 10 μm
Organization
Characteristics		Genome decoded strain (Hori et al. 2014)
Other strain no.		Other collection strain no. : IAM C-117; UTEX 321; SAG 335-1a; CCAP 335/1a
Remarks		Axenic
Movie

Reference
Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730. Strain(s): 2285, 2587, 2860 PubMed: 33293415 DOI: 10.1073/pnas.2005460117 Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108. Keywords: Energy transfer; Nonphotochemical quenching; Photosystem Strain(s): 144, 296, 303, 2285, 2541 PubMed: 30753722 DOI: 10.1093/pcp/pcz026 de Mendoza, A., Bonnet, A., Vargas-Landin, D. B., Ji, N., Li, H., Yang, F., Li, L., Hori, K., Pflueger, J., Buckberry, S., Ohta, H., Rosic, N., Lesage, P., Lin, S., Lister, R. 2018 Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons. Nat. Commun., 9, 1341 (article ID). Keywords: DNA methylation; Epigenomics; Molecular evolution; Transposition Strain(s): 2285 PubMed: 29632298 DOI: 10.1038/s41467-018-03724-9 Ohtaka,K., Hori, K., Kanno, Y., Seo, M., Ohta, H. 2017 Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens. Plant Physiol., 174, 1621-1632. Strain(s): 2285 PubMed: 28533212 DOI: 10.1104/pp.17.00274 Kobayashi, Y., Takusagawa, M., Harada, N., Fukao, Y., Yamaoka, S., Kohchi, T., Hori, K., Ohta, H., Shikanai, T., Nishimura, Y. 2016 Eukaryotic components remodeled chloroplast nucleoid organization during the green plant evolution. Genome Biol. Evol., 8, 1-16. Keywords: chloroplast; nucleoid; evolution; endosymbiosis Strain(s): 2285 PubMed: 26608058 DOI: 10.1093/gbe/evv233 Koeduka, T., Ishizaki, K., Mwenda, C. M., Hori, K., Sasaki-Sekimoto, Y., Ohta, H., Kohchi, T., Matsui, K. 2015 Biochemical characterization of allene oxide synthases from the liverwort Marchantia polymorpha and green microalgae Klebsormidium flaccidum provides insight into the evolutionary divergence of the plant CYP74 family. Planta, 242, 1175-1186. Keywords: Allene oxide synthase; Plant evolution; CYP74; Hydroperoxide lyase; C8 volatiles Strain(s): 2285 PubMed: 26105654 DOI: 10.1007/s00425-015-2355-8 Hori, K. Maruyama, F., Fujisawa, T., Togashi, T., Yamamoto, N., Seo, M., Sato, S., Yamada, T., Mori, H., Tajima, N., Moriyama, T., Ikeuchi, M., Watanabe, M., Wada, H., Kobayashi, K., Saito, M., Masuda, T., Sasaki-Sekimoto, Y., Mashiguchi, K., Awai, K., Shimojima, M., Masuda, S., Iwai, M., Nobusawa, T., Narise, T., Kondo, S., Saito, H., Sato, R., Murakawa, M., Ihara, Y., Oshima-Yamada, Y., Ohtaka, K., Satoh, M., Sonobe, K., Ishii, M., Ohtani, R., Kanamori-Sato, M., Honoki, R., Miyazaki, D., Mochizuki, H., Umetsu, J., Higashi, K., Shibata, D., Kamiya, Y., Sato, N., Nakamura, Y., Tabata, S., Ida, S., Kurokawa, K., Ohta, H. 2014 Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation. Nat. Commun., 5, 3978 (article ID). Strain(s): 2285 PubMed: 24865297 DOI: 10.1038/ncomms4978 Fawley, M. W. 1991 Disjunct distribution of the xanthophyll loroxanthin in the green algae (Chlorophyta). J. Phycol., 27, 544-548. Keywords: carotenoids; Chlorophyta; high-performance liquid chromatography; loroxanthin; xanthophylls Strain(s): 2285 DOI: 10.1111/j.0022-3646.1991.00544.x

Reference

Mojzeš, P., Gao, L., Ismagulova, T., Pilátová, J., Moudříková, Š., Gorelova, O., Solovchenko, A., Nedbal, L., Salih, A. 2020 Guanine, a high-capacity and rapid-turnover nitrogen reserve in microalgal cells. Proc. Natl. Acad. Sci. U. S. A., 117, 32722-32730.
Strain(s): 2285, 2587, 2860
PubMed: 33293415
DOI: 10.1073/pnas.2005460117

Yokono, M., Takabayashi, A., Kishimoto, J., Fujita, T., Iwai, M., Murakami, A., Akimoto, S., Tanaka, A. 2019 The PSI-PSII megacomplex in green plants. Plant Cell Physiol., 60, 1098–1108.
Keywords: Energy transfer; Nonphotochemical quenching; Photosystem
Strain(s): 144, 296, 303, 2285, 2541
PubMed: 30753722
DOI: 10.1093/pcp/pcz026

de Mendoza, A., Bonnet, A., Vargas-Landin, D. B., Ji, N., Li, H., Yang, F., Li, L., Hori, K., Pflueger, J., Buckberry, S., Ohta, H., Rosic, N., Lesage, P., Lin, S., Lister, R. 2018 Recurrent acquisition of cytosine methyltransferases into eukaryotic retrotransposons. Nat. Commun., 9, 1341 (article ID).
Keywords: DNA methylation; Epigenomics; Molecular evolution; Transposition
Strain(s): 2285
PubMed: 29632298
DOI: 10.1038/s41467-018-03724-9

Ohtaka,K., Hori, K., Kanno, Y., Seo, M., Ohta, H. 2017 Primitive Auxin Response without TIR1 and Aux/IAA in the Charophyte Alga Klebsormidium nitens. Plant Physiol., 174, 1621-1632.
Strain(s): 2285
PubMed: 28533212
DOI: 10.1104/pp.17.00274

Kobayashi, Y., Takusagawa, M., Harada, N., Fukao, Y., Yamaoka, S., Kohchi, T., Hori, K., Ohta, H., Shikanai, T., Nishimura, Y. 2016 Eukaryotic components remodeled chloroplast nucleoid organization during the green plant evolution. Genome Biol. Evol., 8, 1-16.
Keywords: chloroplast; nucleoid; evolution; endosymbiosis
Strain(s): 2285
PubMed: 26608058
DOI: 10.1093/gbe/evv233

Koeduka, T., Ishizaki, K., Mwenda, C. M., Hori, K., Sasaki-Sekimoto, Y., Ohta, H., Kohchi, T., Matsui, K. 2015 Biochemical characterization of allene oxide synthases from the liverwort Marchantia polymorpha and green microalgae Klebsormidium flaccidum provides insight into the evolutionary divergence of the plant CYP74 family. Planta, 242, 1175-1186.
Keywords: Allene oxide synthase; Plant evolution; CYP74; Hydroperoxide lyase; C8 volatiles
Strain(s): 2285
PubMed: 26105654
DOI: 10.1007/s00425-015-2355-8

Hori, K. Maruyama, F., Fujisawa, T., Togashi, T., Yamamoto, N., Seo, M., Sato, S., Yamada, T., Mori, H., Tajima, N., Moriyama, T., Ikeuchi, M., Watanabe, M., Wada, H., Kobayashi, K., Saito, M., Masuda, T., Sasaki-Sekimoto, Y., Mashiguchi, K., Awai, K., Shimojima, M., Masuda, S., Iwai, M., Nobusawa, T., Narise, T., Kondo, S., Saito, H., Sato, R., Murakawa, M., Ihara, Y., Oshima-Yamada, Y., Ohtaka, K., Satoh, M., Sonobe, K., Ishii, M., Ohtani, R., Kanamori-Sato, M., Honoki, R., Miyazaki, D., Mochizuki, H., Umetsu, J., Higashi, K., Shibata, D., Kamiya, Y., Sato, N., Nakamura, Y., Tabata, S., Ida, S., Kurokawa, K., Ohta, H. 2014 Klebsormidium flaccidum genome reveals primary factors for plant terrestrial adaptation. Nat. Commun., 5, 3978 (article ID).
Strain(s): 2285
PubMed: 24865297
DOI: 10.1038/ncomms4978

Fawley, M. W. 1991 Disjunct distribution of the xanthophyll loroxanthin in the green algae (Chlorophyta). J. Phycol., 27, 544-548.
Keywords: carotenoids; Chlorophyta; high-performance liquid chromatography; loroxanthin; xanthophylls
Strain(s): 2285
DOI: 10.1111/j.0022-3646.1991.00544.x

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