Strain number NIES-2253  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Dunaliella bioculata Butcher  
Synonym  
Former name  
Common name Green alga  
Locality (Date of collection) Russia  
Latitude / Longitude  
Habitat (Isolation source) Salt water  
History < IAM (2007) < Hara, Yoshiaki (1989) < UTEX (1983-1986)  
Isolator (Date of isolation) Mainx, F.  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  f/2  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information 18S rRNA ( DQ009761 ) , 18S-ITS1-5.8S-ITS2 ( DQ37708 ) , 18S-ITS1-5.8S-ITS2-28S ( KJ094610 ) , ITS1 ( HM035325 DQ182330 ) , ITS2 ( HM035344 DQ157433 ) , 26S rRNA ( DQ015740 ) , rbcL ( DQ313195 ) , ca1 ( X93592 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Authentic strain  
Other strain no. Other collection strain no. : IAM C-523; CCAP 19/4; UTEX LB 199; Prague 281a; SAG 19-4
 
Remarks Axenic 
Movie  
Reference
Becker, B., Melkonian, M. 1991 2-keto-sugar acids in green flagellates: A chemical marker for prasinophycean scales. J. Phycol., 27, 498-504.
Keywords: cell wall composition; Chlorophyta; 2-keto-sugar acids; Prasinophyceae; scale composition
Strain(s): 2253 
DOI: 10.1111/j.0022-3646.1991.00498.x

Schoevaert, D., Krishnamswamy, S., Couturier, M., Marano, F. 1988 Ciliary beat and cell motility of Dunaliella: computer analysis of high speed microcinematography. Biol. Cell, 62, 229-240.
Strain(s): 2253 

Schulze, D., Robenek, H., McFadden, G. I., Melkonian, M. 1987 Immunolocalization of a Ca2+-modulated contractile protein in the flagellar apparatus of green algae: the nucleus-basal body connector. Eur. J. Cell Biol., 45, 51-61.
Strain(s): 2253 

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