Strain number NIES-2237  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas reinhardtii Dangeard  
Synonym Chlamydomonas morieri Dangeard 1888; Chlamydomonas pseudodebaryana Brabez 1941  
Former name  
Common name Green alga  
Locality (Date of collection) near Amherst Massachusetts USA  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Mihara, S. (1991)  
Isolator (Date of isolation)  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  TAP (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB701552 )  
Cell size (min - max) 5 - 18 μm  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; D-Threonine aldolase (Hirato et al. 2007) ; D-amino acids ; Cell wall less mutant of NIES-2238 (IAM C-541) ; Loss of nitrate reductase ; Unable to use nitrate as nitrogen source (Pröschold et al. 2005)  
Other strain no. Other collection strain no. : IAM C-540
Other strain no. : cw-15 strain  
Remarks Cryopreserved; Axenic 
Hirato, Y., Goto, M., Tokuhisa, M., Tanigawa, M., Nishimura, K. 2017 Crystallization and X-ray analysis of D-threonine aldolase from Chlamydomonas reinhardtii. Acta Crystallogr. F-Struct. Biol., F73, 86-89.
Keywords: D-threonine aldolase; Chlamydomonas reinhardtii; D-amino acids; crystallization
Strain(s): 2237 
PubMed: 28177318
DOI: 10.1107/S2053230X1602063X

Hirato, Y., Tokuhisa, M., Tanigawa, M., Ashida, H., Tanaka, H., Nishimura, K. 2017 Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii. Phytochemistry, 135, 18-23.
Keywords: Chlamydomonas reinhardtii; Chlamydomonadaceae; Green alga; Enzyme characterization; d-Amino acid; d-Threonine aldolase; Pyridoxal 5’-phosphate
Strain(s): 2237 
PubMed: 28038776
DOI: 10.1016/j.phytochem.2016.12.012

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Pröschold, T., Harris, E. H., Coleman, A. W. 2005 Portrait of a species: Chlamydomonas reinhardtii. Genetics, 170, 1601-1610.
Keywords: Chlamydomonas; Internal Transcribed Spacer; phylogenetics; population genetics; ribosomal cistrons
Strain(s): 22372238 
PubMed: 15956662
DOI: 10.1534/genetics.105.044503

Kimpel, D. L., Togasaki, R. K., Miyachi, S. 1983 Carbonic anhydrase in Chlamydomonas reinhardtii I. Localization. Plant Cell Physiol., 24, 255-259.
Keywords: carbonic anhydrase; cell wall-less mutant; Chyamydomonas reinhardtii; periplasm; wall lysing enzyme
Strain(s): 22372238 
DOI: 10.1093/pcp/24.2.255

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