Strain number NIES-2223  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Chlamydomonas moewusii Gerloff var. rotunda Tsubo  
Former name  
Common name Green alga  
Locality (Date of collection) Sasayama, Hyogo Japan  
Latitude / Longitude 35.075492 / 135.231221 
Habitat (Isolation source) Freshwater (Paddy soil)  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Tsubo, Yoshihiro (1952-**-**)  
Identified by Tsubo, Yoshihiro  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  2-3 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB701499 )  
Cell size (min - max)  
Organization Unicellular; Flagellate 
Characteristics Phototaxis ; Mating type (+)  
Other strain no. Other collection strain no. : IAM C-556 (=C-16); CCAP 11/64A; UTEX 576; SAG 11-61a
Other strain no. : Tsubo 24 (+)  
Remarks Cryopreserved; Axenic 
Flores, D. C., Fricke, M., Wesp, V., Desirò, D., Kniewasser, A., Hölzer, M., Marz, M., Mittag, M. 2021 A marine Chlamydomonas sp. emerging as an algal model. J. Phycol., 57, 54-69.
Strain(s): 882220222042207221122182220222322292236224122422248 
PubMed: 33043442
DOI: 10.1111/jpy.13083

Yumoto, K., Kasai, F., Kawachi, M. 2013 Taxonomic re-examination of Chlamydomonas strains maintained in the NIES-Collection. Microbiol. Cult. Coll., 29, 1-12.
Strain(s): 158437438440884968102110221048173318481849220122022203220422062207220822092210221122122213221422152216221822192220222222232224222522282229223022312233223422352236223722382239224022412242224322462247224823142315231623172318231923202321232223232324246224632499257525762577257825793284 

Wiese, L., Wiese, W. 1977 On speciation by evolution of gametic incompatibility: a model case in Chlamydomonas. Am. Nat., 111, 733-742.
Strain(s): 2223 
DOI: 10.1086/283202

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