A big image opens when the thumbnail is clicked.(nies-0035.jpg)
Strain number NIES-35  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Raphidocelis subcapitata (Korshikov) Nygaard, Komárek, J.Kristiansen & O.M.Skulberg  
Synonym Ankistrodesmus subcapitatus Korshikov; Kirchneriella subcapitata Korshikov 1953; Kirchneria subcapitata (Korshikov) Hindák 1988; Pseudokirchneriella subcapitata (Korshikov) F.Hindák 1990  
Former name Formerly identified as Pseudokirchneriella subcapitata, re-identified by Krienitz et al. 2011[2018 June]; Selenastrum capricornutum (Printz) Nygaard  
Common name Green alga  
Locality (Date of collection) Nitelva River, Norway  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < Yagi, Osami  
Isolator (Date of isolation) Skulberg, O. M. (1959-**-**)  
Identified by  
State of strain Subculture / Cryopreservation; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  CC (agar)  
Temperature:  20 (25) C
Light intensity:  21 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (14 D)  
Gene information Whole-genome ( BDRX01000000 ) , Mitochondrial DNA ( AP018037 ) , Plastid DNA ( AP018038 ) , rbcL ( LC200423 )  
Cell size (min - max) 5 - 12 μm  
Organization Unicellular 
Characteristics Test strain for AGP ; Genome decoded strain (Suzuki et al. 2018)  
Other strain no. Other collection strain no. : ATCC 22662; CCAP 278/4; SAG 61.81; UTEX 1648
Other strain no. : P-26  
Remarks Axenic 
Movie https://www.youtube.com/watch?v=G_sJ4JDUT4w&index=5&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW  
Oda, Y., Nakamura, H., Tokishita, S., Mano, H., Chang, K.-H., Sakamoto, M. 2021 Phenotypic changes in Daphnia pulex under oxygen deficiency, resource limitation and predation risk. Ecol. Res., 36, 533-544.
Keywords: Daphnia pulex; filter‐screen enlargement; hemoglobin synthesis; neck teeth formation; phenotypic plasticity
Strain(s): 35 
DOI: 10.1111/1440-1703.12216

Wan, J.-K., Chu, W.-L., Kok, Y.-Y., Lee, C.-S. 2021 Influence of polystyrene microplastic and nanoplastic on copper toxicity in two freshwater microalgae. Environ. Sci. Pollut. Res., 28, 33649–33668.
Keywords: Microplastic; Nanoplastic; Copper; Chlorella; Pseudokirchneriella subcapitata; Combined toxicity; Adsorption
Strain(s): 35 
PubMed: 33646549
DOI: 10.1007/s11356-021-12983-x

Wada, S., Natori, K. 2021 Practice of class to measure the environmental load of antibacterial drugs by student planning experiment. -Antibiotics suppress germination and growth of plants- JSSE Research Report, 35, 55-60 (in Japanese).
Strain(s): 35 
DOI: 10.14935/jsser.35.8_55

Mizukami-Murata, S., Suzuki, Y., Sakurai, K., Yamashita, H. 2021 Freshwater alga Raphidocelis subcapitata undergoes metabolomic changes in response to electrostatic adhesion by micrometer-sized nylon 6 particles. Environ. Sci. Pollut. Res., ,
Keywords: Powdered nylon 6; Raphidocelis subcapitata; Growth inhibition; Electrostatic adhesion; Metabolomic analysis; Amino acid catabolic pathway; γ-glutamyl cycle
Strain(s): 35 
PubMed: 34236613
DOI: 10.1007/s11356-021-15300-8

Wada, S., Kumamoto, T., Yamamoto, Y., Nakasuga, H., Koike, K., Horie, H. 2020 Development of the experimental teaching materials to easily observe the environmental effects of antimicrobaials. JSSE Research Report, 34, 1-15 (in Japanese).
Strain(s): 35 
DOI: 10.14935/jsser.34.7_39

Sakamoto, M., Kashiwada, S. 2020 Toxicity of silver nanocolloids to Raphidocelis subcapitata. Jpn. J. Environ. Toxicol., 23, 63-66 (in Japanese with English summary).
Strain(s): 35 
DOI: 10.11403/jset.23.63

Kamei-Ishikawa, N., Maeda, T., Soma, M., Yoshida, N., Sameshima, Y., Sasamoto, M., Higashiyama, Y., Touno, E., Ito, A. 2020 Tylosin degradation during manure composting and the effect of the degradation byproducts on the growth of green algae. Sci. Total Environ., 718, 137295 (article ID).
Keywords: Sheep feces; Half-life; Thermal degradation; Elution from artificial rainwater; Acute toxicity test; Raphidocelis subcapitata
Strain(s): 35 
DOI: 10.1016/j.scitotenv.2020.137295

Pascual, G., Sano, D., Sakamaki, T., Nishimura, O. 2020 Effects of chemical interaction of nutrients and EDTA on metals toxicity to Pseudokirckneriella subcapitata. Ecotox. Environ. Safe., 203, 110966 (article ID).
Keywords: Metal toxicity; Nutrient metal; Non-nutrient metal; EDTA; Metal speciation
Strain(s): 35 
PubMed: 32678755
DOI: 10.1016/j.ecoenv.2020.110966

Yamagishi, T., Yamaguchi, H., Suzuki, S., Yoshikawa, M., Jameson, I., Lorenz, M., Nobles, D. R., Campbell, C., Seki, M., Kawachi, M., Yamamoto, H. 2020 Comparative genome analysis of test algal strain NIVA-CHL1 (Raphidocelis subcapitata) maintained in microalgal culture collections worldwide. PLoS One, 15, e0241889 (article ID).
Strain(s): 35 
PubMed: 33166324
DOI: 10.1371/journal.pone.0241889

Widyaningrum, D., Iida, D., Tanabe, Y., Hayashi, Y., Kurniasih, S. D., Ohama, T. 2019 Acutely induced cell mortality in the unicellular green alga Chlamydomonas reinhardtii (Chlorophyceae) following exposure to acrylic resin nanoparticles. J. Phycol., 55, 118-133.
Keywords: abnormal swimming behavior; cell mortality; cell wall damage; Chlamydomonas rein-hardtii; poly(isobutyl-cyanoacrylate) nanoparticles; ROS
Strain(s): 3514436241842143243845654557173273785810221048184821502169220122022208220922122280235224622578 
PubMed: 30304548
DOI: 10.1111/jpy.12798

Religia, P., Kato, Y., Fukushima, E. O., Matsuura, T., Muranaka, T., Watanabe, H. 2019 Atrazine exposed phytoplankton causes the production of non-viable 2 offspring on Daphnia magna. Mar. Environ. Res., 145, 177-183.
Keywords: Atrazine; Daphnia magna; Raphidocelis subcapitata; Risk assessment; Sublethal effects
Strain(s): 35 
DOI: 10.1016/j.marenvres.2019.02.007

Chin, Y.-Y., Chu, W.-L., Kok, Y.-Y., Phang, S.-M., Wong, C.-Y., Tan, B.-K., Mustafa, E. M. 2019 Sensitivity of selected tropical microalgae isolated from a farmland and a eutrophic lake to atrazine and endosulfan. J. Appl. Phycol., 31, 2981–299.
Keywords: Atrazine; Endosulfan; Microalgae; Oxidative stress; Chlorella; Scenedesmus
Strain(s): 35 
DOI: 10.1007/s10811-019-01800-1

Kashem, MA., Kimoto, K., Iribe, Y., Suzuki, M. 2019 Development of microalgae biosensor chip by incorporating microarray oxygen sensor for pesticides sensing. Biosensors, 9, 133 (article ID).
Keywords: biosensor chip; fluorescence oxygen sensor; microalgae; pesticides
Strain(s): 35 
PubMed: 31726653
DOI: 10.3390/bios9040133

Koga, T., Kashiwabara, M., Hirakawa, S., Shimizu, N., Ishibashi, Y. 2019 Development of an algal growth inhibition test as small-scale method for testing whole effluent toxicity. J. Environ. Chem., 29, 67-77 (in Japanese with English summary).
Keywords: whole effluent toxicity; algal growth inhibition; small-scale method
Strain(s): 35 
DOI: 10.5985/jec.29.67

Suzuki, S., Yamaguchi, H., Nakajima, N., Kawachi, M. 2018 Raphidocelis subcapitata (=Pseudokirchneriella subcapitata) provides an insight into genome evolution and environmental adaptations in the Sphaeropleales. Sci Rep, 8, 8058 (article ID).
Strain(s): 35 
PubMed: 29795299
DOI: 10.1038/s41598-018-26331-6

Tasmin, R., Shimasaki, Y., Tsuyama, M., Qiu, X., Khalil, F., Mukai, K., Khanam, M. R. M., Yamada, N., Fukuda, S., Kang, I. J., Oshima, Y. 2018 Effects of water temperature and light intensity on the acute toxicity of herbicide thiobencarb to a green alga, Raphidocelis subcapitata. Environ. Sci. Pollut. Res., 25, 25363–25370.
Keywords: Thiobencarb; Raphidocelis subcapitata; Watertemperature; Lightintensity; Photosystem II; Effectiveconcentration
Strain(s): 35 
PubMed: 29946846
DOI: 10.1007/s11356-018-2599-5

Jin, J., Zhang, X., Kusui, T. 2018 Preliminary toxicity Assessment of combined Sewer overflows in Toyama, Japan. J. Water Environ. Technol., 16, 185-198.
Keywords: combined sewer overflows (CSOs); toxicity identification evaluation (TIE); toxicity test; heavy metals; aquatic organisms
Strain(s): 35 
DOI: 10.2965/jwet.17-041

Hamana, K., Kobayashi, M., Furuchi, T., Hayashi, H., Niitsu, M. 2018 Polyamine analysis of unicellular, colonial, and multicellular green algae —Detection of aminobutylcadaverine, N1-aminopentylspermidine, N8-aminopentylspermidine, and penta-amines.— Microb. Resour. Syst., 34, 73-82.
Keywords: aminobutylcadaverine; aminopentylspermidine; green alga; penta-amine; polyamine; thermospermine
Strain(s): 1835242691735127112782185218722352261254025723983 

Yeo, B.-S., Chu, W.-L., Wong, C.-Y., Kok, Y.-Y., Phang, S.-M., Tan, B.-K., Mustafa, E. M. 2018 Combined effects of glufosinate ammonium and temperature on the growth, photosynthetic pigment content and oxidative stress response of Chlorella sp. and Pseudokirchneriella subcapitata. J. Appl. Phycol., 30, 3043–3055.
Keywords: Glufosinate ammonium; Temperature; Herbicides; Chlorella; Pseudokirchneriella subcapitata
Strain(s): 35 
DOI: 10.1007/s10811-018-1613-0

Takeda, F., Suzuki, M., Okamoto, S., Minamiyama, M. 2018 Evaluation of four types of biological treatment processes in wastewater treatment plants with regard to reducing ability of growth-inhibiting effect on alga in summer and winter and characterization of algal growth-inhibiting chemicals. J. Jpn. Soc. Wat. Environ., 41, 179-191 (in Japanese with English summary).
Keywords: Whole effluent toxicity test; Toxicity identification evaluation; Pseudokirchneriella subcapitata; Wastewater management
Strain(s): 35 
DOI: 10.2965/jswe.41.179

Yamagishi, T., Yamaguchi, H., Suzuki, S., Horie, Y., Tatarazako, N. 2017 Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata (=Selenastrum capricornutum) and their variations under exposure to the typical toxicants potassium dichromate and 3,5-DCP. PLoS One, 12, e0171259 (article ID).
Strain(s): 35 
PubMed: 28152022
DOI: 10.1371/journal.pone.0171259

Yamagishi, T., Horie, Y., Tatarazako, N. 2017 Synergism between macrolide antibiotics and the azole fungicide ketoconazole in growth inhibition testing of the green alga Pseudokirchneriella subcapitata. Chemosphere, 174, 1-7.
Keywords: Azole fungicide; Clarithromycin; Erythromycin; Isobologram; Ketoconazole; Pseudokirchneriella subcapitata
Strain(s): 35 
PubMed: 28152378
DOI: 10.1016/j.chemosphere.2017.01.071

Takeda, F., Minamiyama, M., Okamoto, S. 2017 Seasonal variation in ability of wastewater treatment for reduction in biological effects evaluated based on algal growth. J. Water Environ. Technol., 15, 96-105.
Keywords: pollutant release and transfer register (PRTR) information; Pseudokirchneriella subcapitata; toxicity identification evaluation (TIE); wastewater treatment; whole effluent toxicity (WET)
Strain(s): 35 
DOI: 10.2965/jwet.16-051

Tamura, I., Yasuda, Y., Kagota, K., Yoneda, S., Nakada, N., Vimal Kumar, V., Yutaka Kameda, Y., Kumiko Kimura, K., Norihisa Tatarazako, N., Hiroshi Yamamoto, H. 2017 Contribution of pharmaceuticals and personal care products (PPCPs) to whole toxicity of water samples collected in effluent-dominated urban streams. Ecotox. Environ. Safe., 144, 338-350.
Keywords: PPCPs; Anionic surfactant; Whole toxicity; Bioassay
Strain(s): 35 
PubMed: 28646739
DOI: 10.1016/j.ecoenv.2017.06.032

Katsumata, M., Ikushima, Y., Bennett, K., Sato, Y., Takeuchi, A., Tatarazako, N., Hakamata, T. 2017 Validation of rapid algal bioassay using delayed fluorescence in an interlaboratory ring study. Sci. Total Environ., 605-606, 842-851.
Keywords: Chemical toxicity test; Growth inhibition; Alga; Delayed fluorescence; Surrogate of biomass
Strain(s): 35 
PubMed: 28683428
DOI: 10.1016/j.scitotenv.2017.06.228

Mano, H., Takeda, F., Kitamura, T., Okamoto, S., Suzuki, Y., Park, CB., Yasui, N., Kobayashi, K., Tanaka, Y., Yamashita, N., Minamiyama, M. 2017 Water quality comparison of secondary effluent and reclaimed water to ambient river water of southern Okinawa Island via biological evaluation. Environ. Monit. Assess., 189, 442 (article ID).
Keywords: Bioassay; Ecological risk; Feasibility study; Water reclamation; Water reuse
Strain(s): 35 
PubMed: 28791526
DOI: 10.1007/s10661-017-6160-7

Ando, D., Fujisawa, T., Katagi, T. 2017 Fate of flumioxazin in aquatic plants: Two algae (Pseudokirchneriella subcapitata, Synechococcus sp.), duckweed (Lemna sp.), and water milfoil (Myriophyllum elatinoides). J. Agric. Food Chem., 65, 8813-8822.
Keywords: aquatic plants; conjugation; flumioxazin; metabolism; sediment exposure; water exposure
Strain(s): 35937 
PubMed: 28920683
DOI: 10.1021/acs.jafc.7b03355

Kusui, T., Itatsu, Y., Jin, J. 2017 Whole effluent toxicity assessment of industrial effluents. In Toxicity and biodegradation testing, Eds. by Bidoia, E. D. & Montagnolli, R. N., Humana Press, New York, NY, pp. 331-347.
Strain(s): 35 
DOI: 10.1007/978-1-4939-7425-2_17

Yamashita, N., Fukunaga, A., Tanaka, H. 2017 Examination of microplate AGP test using green algae, diatom and blue-green algae and water quality evaluation of river water and treated sewage effluent by AGP test. Environmental science, 30, 250-260 (in Japanese with English summary).
Keywords: Algal growth potential; mircoplate; bioassay; eutrophication; water quality evaluation
Strain(s): 3590805 
DOI: 10.11353/sesj.30.250

Watanabe, H., Tamura, I., Abe, R. Takanobu, H., Nakamura, A., Suzuki, T., Hirose, A., Nishimura, T., Tatarazako, N. 2016 Chronic toxicity of an environmentally relevant mixture of pharmaceuticals to three aquatic organisms (alga, daphnid, and fish). Environ. Toxicol. Chem., 35, 996-1006.
Keywords: Mixture toxicity; Pharmaceutical; Short-term chronic toxicity test; Concentration addition; Independent action
Strain(s): 35 
PubMed: 26472177
DOI: 10.1002/etc.3285

Yagi, Satoshi, Kawakami, H., Fujiwara, T., Ishikawa, N., Ito, A., Sasamoto, M., Umita, T. 2016 Individual and combined toxicity of antibacterial substances to green alga Pseudokirchneriella subcapitata. J. Jpn. Soc. Wat. Environ., 3, 63-70 (in Japanese with English summary).
Keywords: Green algar; EC50; Toxicity test; Antibacterial substance; Combined toxicity
Strain(s): 35 
DOI: 10.2965/jswe.39.63

Mano, H., Okamoto, S. 2016 Preliminary ecological risk assessment of 10 PPCPs and their contributions to the toxicity of concentrated surface water on an algal species in the middle basin of Tama River. J. Water Environ. Technol., 14, 423-436.
Keywords: bioassay; environmental risk; hazard quotien
Strain(s): 35 
DOI: 10.2965/jwet.15-045

Yachi, S., Nagai, T., Katsumata, M. 2016 Variation in delayed fluorescence of green algae and diatom during the exposure and a subsequent recovery period. Jpn. J. Environ. Toxicol., 19, 35-46 (in Japanese with English summary).
Keywords: Recovery test; Physiological state of algae; 3,5-DCP
Strain(s): 35 
DOI: 10.11403/jset.19.35

Mori, I. C., Arias-Barreiro, C. R., Koutsaftis, A., Ogo, A., Kawano, T., Yoshizuka, K., Inayat-Hussain, S. H., Aoyama, I. 2015 Toxicity of tetramethylammonium hydroxide to aquatic organisms and its synergistic action with potassium iodide. Chemosphere, 120, 299-304.
Keywords: Tetramethylammonium hydroxide; Potassium iodide; Aquatic toxicity; Synergism; D. magna; Semiconductor wastewater
Strain(s): 35 
PubMed: 25151133
DOI: 10.1016/j.chemosphere.2014.07.011

Mano, H., Takeda, F., Kitamura, T., Okamoto, S., Kobayashi, K., Takabatake, H., Yamashita, N., Tanaka, H. 2015 Evalutation of toxicity reduction of treated wastewater by NF and RO membrane technologies based on algal growth inhibition test and short term toxicity test on embryo and sac-fry stages of Medaka. J. Jpn. Soc. Civill Engineers, Ser. G (Environmental Research), 71, III 179-III 187(in Japanese with English summary).
Keywords: bioassay; nanofiltration membrane; reclaimed water; reverse osmosis membrane; toxicity
Strain(s): 35 
DOI: 10.2208/jscejer.71.III_179

Itatsu, Y., Takano, T., Jin, J., Fukutomi, M., Kusui, T. 2015 Ecotoxicological assessment of industrial effluents: Toxicity characterization and impact on receiving water. J. Environ. Chem., 25, 19-26 (in Japanese with English summary).
Keywords: whole effluent toxicity; industrial effluent; toxicity characterization
Strain(s): 35 
DOI: 10.5985/jec.25.19

Tasmin, R., Shimasaki, Y., Tsuyama, M., qiu, X., Khalil, F., Okino, N., Yamada, N., Fukuda, S., Kang, I.-J., Oshima, Y. 2014 Elevated water temperature reduces the acute toxicity of the widely used herbicide diuron to a green alga, Pseudokirchneriella subcapitata. Environ. Sci. Pollut. Res., 21, 1064-1070.
Keywords: Diuron; Pseudokirchneriella subcapitata; Water temperature; Photosynthetic activity; Intracellular H2O2.
Strain(s): 35 
PubMed: 23872901
DOI: 10.1007/s11356-013-1989-y

Kusumi, T., Takata, Y., Itatsu, Y., Zha, J. 2014 Whole effluent toxicity assessment of industrial effluents in Toyama, Japan with a battery of short-term chronic bioassays. J. Water Environ. Technol., 12, 55-63.
Keywords: aqquatic organism; ecotoxic effect; industrial effluent; whole effluent toxicity (WET)
Strain(s): 35 
DOI: 10.2965/jwet.2014.55

Takeda, F., Nakano, K., Aikawa, Y., Nishimura, O., Shimada, Y., Fukuro, S., Nakazawa, T., Tanaka, H., Hayashi, N., Inamori, Y. 2014 Analysis of impact of allelopathy by submerged macrophytes simulated by ecosystem model on microalgal biomass and community. J. Jpn. Soc. Wat. Environ., 15, 15-28 (in Japanese with English summary).
Keywords: Allelopathy; Potamogeton pusillus; E cosystem model; M icroalgal community; Cladoceran
Strain(s): 3587 
DOI: 10.2965/jswe.37.15

Takeuchi, A., Katsumata, M., Koike, T., Takata, Y., Itatsu, Y., Kusui, T. 2014 Comparison of the conventional algal growth inhibition tests using cell counting and algal bioassay using delayed fluorescence: application to industrial effluents. J. Water Environ. Technol., 12, 367-377.
Keywords: algal growth inhibition test; delayed fluorescence; rapid bioassay; whole effluent toxixity
Strain(s): 35 
DOI: 10.2965/jwet.2014.367

Nakano, S., Chang, K.-H., Shijima, A., Miyamoto, H., Sato, Y., Noto, Y., Ha, J.-Y., Sakamoto, M. 2014 A usage of CO2 hydrate: Convenient method to increase CO2 concentration in culturing algae. Bioresour. Technol., 172, 444-448.
Keywords: CO 2; Algal culture; Pseudokirchneriella; Population growth
Strain(s): 35 
PubMed: 25263943
DOI: 10.1016/j.biortech.2014.09.019

Nagai, T., Kamo, M. 2014 Comparative modeling of the effect of metal mixtures on algal growth using biotic ligand model, concentration additive, and independent action. Jpn. J. Environ. Toxicol., 17, 57-68 (in Japanese with English summary).
Keywords: Zinc; Copper; Cadmium; Metal speciation; Ecological risk assessment
Strain(s): 35 
DOI: 10.11403/jset.17.57

Nagai, T., Taya, K., Annoh, H., Ishihara, S. 2013 Application of a fluorometric microplate algal toxicity assay for riverine periphytic algal species. Ecotox. Environ. Safe., 94, 37-44.
Keywords: Attached algae; In-vivo fluorescence; Pesticides; Species sensitivity distribution
Strain(s): 357173414487512797 
DOI: 10.1016/j.ecoenv.2013.04.020

Shoji, R., Okita, N., nakanishi, H. 2013 Analysis of the effects of humic acid extracter from the Tama River sediment on the Pseudokirchneriella subcapitata growth inhibition by copper by NICA (non-ideal competitive adsorption)-Donnan model. J. Jpn. Soc. Wat. Environ., 36, 1-9 (in Japanese with English summary).
Keywords: River sediment; Ecotoxicity; Heavy metal
Strain(s): 35 
DOI: 10.2965/jswe.36.1

Tamura, I., Kagota, K., Yasuda, Y., Yoneda, S., Morita, J., Nakada, N., Kameda, Y., Kimura, K., Tatarazako, N., Yamamoto, H. 2013 Ecotoxicity and screening level ecotoxicological risk assessment of five antimicrobial agents: triclosan, triclocarban, resorcinol, phenoxyethanol and p-thymol. J. Appl. Toxicol., 33, 1222-1229
Keywords: antimicrobial agents; triclosan; triclocarban; phenoxyethanol; ecotoxicity; green algae; Ceriodaphnia dubia; ecological risk
Strain(s): 35 
PubMed: 2806922
DOI: 10.1002/jat.2771

Nagai, T., Horio, T., Yokoyama, A., Kamiya, T., Takano, H., Makino, T. 2012 Ecological risk assessment of on-site soil washing with iron(III) chloride in cadmium-contaminated paddy field. Ecotox. Environ. Safe., 80, 84-90.
Keywords: Risk assessment; Soil washing; Bioassay; Species sensitivity distribution
Strain(s): 35 
PubMed: 22377402
DOI: 10.1016/j.ecoenv.2012.02.011

Ioki, M., Ohkoshi, M., Nakajima, N., Nakahira-Yanaka, Y., Watanabe, M. M. 2012 Isolation of herbicide-resistant mutants of Botryococcus braunii. Bioresour. Technol., 109, 300-303.
Keywords: Botryococcus braunii; Paraquat; Methyl viologen; Basta; Glufosinate
Strain(s): 351192272302235 
PubMed: 21906932
DOI: 10.1016/j.biortech.2011.07.101

Suda, T., Hata, T., Kawai, S., Okamura, H., Nishida, T. 2012 Treatment of tetracycline antibiotics by laccase in the presence of 1-hydroxybenzotriazole. Bioresour. Technol., 103, 498-501.
Keywords: Tetracycline antibiotics; Bacterial growth inhibition; Algal growth inhibition; Laccase; 1-Hydroxybenzotriazole
Strain(s): 35 
PubMed: 22071243
DOI: 10.1016/j.biortech.2011.10.041

Morita, M., Murayama, K., Komori, K., Minamiyama, M. 2012 Evaluation of levofloxacin in chlorination by algal growth inhibition. J. Environ. Chem., 22, 183-192 (in Japanese with English summary).
Keywords: hydrogen peroxide; marine ecotoxicity; seaweed gametophytes; phytoplankton; zooplankton
Strain(s): 35 
DOI: 10.5985/jec.22.183

Nagai, T., Ishihara, S., Yokoyama, A., Iwafune, T. 2011 Effects of four rice paddy herbicides on algal cell viability and the relationship with population recovery. Environ. Toxicol. Chem., 30, 1898-1905.
Keywords: pesticide; ecological risk; flow cytometry; green algae
Strain(s): 35 
PubMed: 21590715
DOI: 10.1002/etc.582

Inoue, Y., Hata, T., Kawai, S., Okamura,H., Nishida, T. 2010 Elimination and detoxification of triclosan by manganese peroxidase from white rot fungus. J. Hazard. Mater., 180, 764-767.
Keywords: Triclosan; Bacterial growth inhibition; Algal growth inhibition; Manganese peroxidase; Ligninolytic enzymes; White rot fungi
Strain(s): 35 
PubMed: 20434837
DOI: 10.1016/j.jhazmat.2010.04.024

Shoji, R., Nkayama, H., Sakai, Y., Mohri, S., Yamada, M. 2008 Evaluation of the ecotoxicity of solid wastes using rapid leaching test and bioassays. J. Environ. Sci. Health Part A-Toxic/Hazard. Subst. Environ. Eng. , 43, 1048-1053.
Keywords: Heavy metals; ecotoxicity; organic compounds; rapid leaching test; bioassays
Strain(s): 35 
PubMed: 8569319
DOI: 10.1080/10934520802059995

Murakami, M., Sato, N., Anegawa, A., Nakada, N., Harada, A., Komatsu, T., Takada, H., Tanaka, H., Ono, Y., Furumai, H. 2008 Multiple evaluations of the removal of pollutants in road runoff by soil infiltration. Water Res., 42, 2745-2755.
Keywords: Groundwater contamination; Infiltration facility; Road dust; Urban runoff; Soil infiltration column test; Water-soluble micropollutants
Strain(s): 35 
DOI: 10.1016/j.watres.2008.02.004

Yamamoto, H., Nakamura, Y., Nakamura, Y., Kitani, C., Imari, T., Sekizawa, J., Takao, Y., Yamashita, N., Hirai, N., Oda, S., Takarazako, N. 2007 Initial ecological risk assessment of eight selected human pharmaceuticals in Japan. Environ. Sci., 14, 177-193.
Keywords: pharmaceuticals; initial risk assessment; aquatic organisms; predicted no effect concentration
Strain(s): 35 
PubMed: 17762841

Nakajima, N., Teramoto, T., Kasai, F., Sano, T., Tamaoki, M., Aono, M., Kubo, A., Kamada, H., Azumi, Y., Saji, H. 2007 Glycosylation of bisphenol A by freshwater microalgae. Chemosphere, 69, 934-941.
Keywords: Metabolite; Glucoside; Galactoside; Endocrine disruptors
Strain(s): 359496151245424468763 
PubMed: 17629547
DOI: 10.1016/j.chemosphere.2007.05.088

Takei, T., Odake, H., Miura, K., Takagi, Y. 2006 Ecotoxicity of tetradecanoic acid, 2-sulfo-, 1-metylester, sodium salt (C14MES). J. Oleo Sci., 55, 121-126.
Keywords: MES; ecotoxicity; alga; daphnia; reproduction
Strain(s): 35 
DOI: 10.5650/jos.55.121

Yamashita, N., Yasojima, M., Nakada, N., Miyajima, K., Komori, K., Suzuki, Y., Tanaka, H. 2006 Effects of antibacterial agents, levofloxacin and clarithromycin, on aquatic organisms. Water Sci. Technol., 53, 65-72.
Keywords: antibacterial agent; clarithromycin; ecotoxicity; levofloxacin; risk
Strain(s): 35 
PubMed: 16862775
DOI: 10.2166/wst.2006.338

Yamashita, N., Tanaka, H., Miyajima, K., Suzuki, Y. 2006 Evaluation of effect of seaage effluent on river water quality using AGP and algal growth inhibition tests. J. JSCE, 62, 191-200 (in Japanese with English summary).
Keywords: AGP test; algal growth inhibition test; sewage effluent; Tama River
Strain(s): 35 
DOI: 10.2208/jscejg.62.191

Hatakeyama, S. 2006 Assessment of overall herbicide effects on river ecosystems through periphyton and aquatic plants. Jpn. J. Environ. Toxicol., 9, 51-60 (in Japanese with English title).
Strain(s): 35 
DOI: 10.11403/jset.9.51

Katsumata, M., Koike, T., Nishikawa, M., Kazumura, K., Tsuchiya, H. 2006 Rapid ecotoxicological bioassay using delayed fluorescence in the green alga Pseudokirchneriella subcapitata. Water Res., 40, 3393-3400.
Keywords: Delayed fluorescence; Algal growth inhibition test; Pseudokirchneriella subcapitata; Simazine (CAT); 3,5-dichlorophenol (3,5-DCP)
Strain(s): 35 
PubMed: 16970970
DOI: 10.1016/j.watres.2006.07.016

Kamaya,Y., Tsuboi, S., Takada, T., Suzuki, K. 2006 Growth stimulation and inhibition effects of 4-hydroxybenzoic acid and some related compounds on the freshwater green alga Pseudokirchneriella subcapitata. Arch. Environ. Contam. Toxicol., 51, 537-541.
Strain(s): 35 
DOI: 10.1007/s00244-005-0320-4

Kaneko, H., Shimada, A. Hirayama, K. 2004 Short-term algal toxicity test based on phosphate uptake. Water Res., 38, 2173-2177.
Keywords: Selenastrum capricornutum; Phosphate uptake; Short-term toxicity test
Strain(s): 35 
PubMed: 15087199
DOI: 10.1016/j.watres.2004.02.006

Ando, T., Otsuka, S., Nishiyama, M., Sendo, K., Watanabe, M. M., Matsumoto, S. 2003 Toxic effects of dichloromethane and trichloroethlene on the growth of planktonic green algae, Chlorella vulgaris NIES227, Selenastrum capricornutum NIES35, and Volvulina steinii NIES545. Microbes Environ., 18, 43-46.
Keywords: algal growth potential (AGP); bioindicator; dichloromethane; organic solvents; trichloroethlene
Strain(s): 35227545 
DOI: 10.1264/jsme2.18.43

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19212223242526272829303132333435363738394041444546505152535455565758596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123124125127128129130131132133134135137138139144147148150151152153154155156157158159160162163164165166167168169170171172173174175176177178179180181182183185186187188189191192193194195197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268271287288289290294295297298299300301302303305306308309310311312313329334336337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Okamura, H., Piao, M., Aoyama, I., Sudo, M., Okubo, T., Nakamura, M. 2002 Algal growth inhibition by river water pollutants in the agricultural area around Lake Biwa, Japan. Environ. Pollut., 117, 411-419.
Keywords: Algal bioassay; Herbicide; Lake Biwa; Runoff; Simetryn
Strain(s): 35 
DOI: 10.1016/S0269-7491(01)00196-8

Tatarazako, N., Yamamoto, K., Iwasaki, K. 2002 Subacute toxicity of wood preservatives, DDAC and BAAC, in several aquatic organisms. J. Health Sci., 48, 359-365.
Keywords: biological test; wood preservative; chronic toxicity; daphnia; green algae; fish
Strain(s): 35 
DOI: 10.1248/jhs.48.359

Sasao, A., Matsuo, H., Tanaka, Y. 2001 Inhibitory effect of tea (Camellia sinessis (L.) O.Kuntze var. sinensis) liquor / extract on blue-green algal growth. Jpn. J. Limnol., 62, 115-122 (in Japanese with English summary).
Keywords: tea; M. aeruginosa; growth inhibitor; tannic acid
Strain(s): 35 
DOI: 10.3739/rikusui.62.115

Nakai, S., Yamane, S., Hosomi, M. 2000 Algal growth inhibition effects of Myriophyllum spicatum-releasing four allelopathic polyphenols. J. Jpn. Soc. Wat. Environ., 23, 726-730 (in Japanese with English summary).
Keywords: allelochemical; polyphenol; algae; concentration-dependent synergistic growth inhibition; autoxidation
Strain(s): 35718796489512 

Okamura, H., Aoyama, I., Liu, D., Maguire, R. J., Pacepavicius, G. J., Lau, Y. L. 2000 Fate and ecotoxicity of the new antifouling compound irgarol 1051 in the aquatic environment. Water Res., 34, 3523-3530.
Keywords: antifouling compound; degradation product; ecotoxicity; fate; berbicide; Irgarol 1051; M1; Seto Inland Sea Japan; transformation
Strain(s): 35 
DOI: 10.1016/S0043-1354(00)00095-6

Kaneko, H., Takeuchi, H., Hirayama, K. 2000 Development of short-term algal toxicity test using oxygen evolution rate. Jpn. J. Environ. Toxicol., 3, 3-9 (in Japanese with English summary).
Keywords: algal toxicity test; oxygen evolution; short-term bioassay; sensitivity
Strain(s): 35 
DOI: 10.11403/jset.3.3

Nishida, T., Tsutsumi, Y., Kemi, M., Haneda, T., Okamura, H. 1999 Decolorization of anthraquinone dyes by white-rot fungi and its related enzymes. J. Jpn. Soc. Wat. Environ., 22, 465-471 (in Japanese with English summary).
Keywords: anthraquinone dye; white-rot fungi; laccase; decolorization; toxicity
Strain(s): 35 

Ono, Y., Okamura,H., Kawara, O., Aoyama, I., Oda, Y. 1999 Genotoxicity and ecotoxicity of sediment in road runoff. J. Jpn. Soc. Wat. Environ., 22, 561-567 (in Japanese with English summary).
Keywords: road runoff; road sediment; genotoxicity; growth inhibition; acute toxicity
Strain(s): 35 

Kaneko, H. 1997 Toxicity of municipal waste incinerator fly ash using algal growth. J. Jpn.n Soc. Waste Management Experts, b>8, 321-326 (in Japanese with English summary).
Keywords: MWI fly ash; leaching test; ecotoxicity; algal assay; heavy metal
Strain(s): 35 
DOI: 10.3985/jswme.8.321

Kaneko, H., Yamaguchi, M., Ikeda, F. 1996 Toxicity of municipal solid waste incinerator fly ash and the contribution of Cadmium. J. Jpn. Soc. Wat. Environ. [Mizukankyô Gakkai Shi], 19, 563-568 (in Japanese with English summary).
Keywords: MWI fly ash; leachate; algal assay; Daphnia test; hazardous waste
Strain(s): 35 

Kaneko, H., Yamaguchi, M. 1995 Toxicity evaluation of municipal solid waste incinerator fly ash by algal assay. Reports Facul. Eng. Yamanashi Univ., 46, 84-89 (in Japanese with English summary).
Strain(s): 35 

Nakai, S., Inoue, H., Hosomi, M., Okada, M., Murakami, A. 1995 Control of phytoplankton growth by bioactive compounds extracted from macrophytes. J. Jpn. Soc. Wat. Environ., 18, 1012-1019 (in Japanese with English summary).
Keywords: macrophyte; algae; allelopathy; antialgal bioactive compound; growth inhibition
Strain(s): 3587 
DOI: 10.2965/jswe.18.1012

Hatakeyama, S., Fukushima, S., Kasai, F., Shiraishi, H. 1994 Assessment of herbicide effects on algal production in the Kokai River (Japan) using a model stream and Selenastrum bioassay. Ecotoxicology, 3, 143-156.
Keywords: herbicides; effects; algal production; algal community; channel carrying river water; bioassay; pesticide analysis
Strain(s): 35 

Kasai, F., Hatakeyama, S. 1993 Herbicide susceptibility in two green algae, Chlorella vulgaris and Selenastrum capricornutum. Chemosphere, 27, 899-904.
Strain(s): 35227 
DOI: 10.1016/0045-6535(93)90019-2

Kasai, F., Takamura, N., Hatakeyama, S. 1993 Effects of simetryne on growth of various freshwater algal taxa. Environ. Pollut., 79, 77-83.
Strain(s): 35447377102137151230231255275280298 
DOI: 10.1016/0269-7491(93)90180-V

Nakamura, H. 1993 Results of AGP experiments [AGP, T-P, PO4-P no shiken oyobi bunsekikekka]. Unpublished report in Ebara Yujiraito Co. Ltd,. 32 pp. (in Japanese).
Strain(s): 35101 

Noike, T., Souma, Y. 1993 Survey on the influence factors of eutrophication of lakes and the necessity of advanced waste treatment [Koshô no fu-eiyôka no kanôsei to kôdo shori no hitsuyôsei ni kansuru chôsa]. J. Jpn. Soc. Sewage Works [Gesuidô-Kyôkai Shi], 30, 38-44 (in Japanese).
Strain(s): 35 

Tsukui, T., Yamazaki, M. 1992 Studies on eutrophication in rivers (II). Determination of AGP in the Kanda River System. Tokyo-to Kankyô Kagaku Kenkyûjo Nenpô 1992, 162-166 (in Japanese with English Summary).
Strain(s): 35 

Kawanobe, K. 1990 A report in the completion of the research work [Chôsa kenkyû syûryô hôkoku]. Unpublished report in Kankyô Hozen Kyôkai, 3 pp. (in Japanese).
Strain(s): 35227 

Yagi, O., Ohkubo, N., Tomioka, N., Okada, M. 1989 Limiting nutrients of algal growth in Ushiku Marsh. Jpn. J. Limnol., 50, 139-148 (in Japanese with English summary).
Keywords: algal growth potential; Microcystis; Selenastrum; nutrient
Strain(s): 35478 

Nakahara, M., Ito, H., Yano, H., Harimaya, K. 1988 Eutrophication in three reservoirs as the sources of the waterworks in Kobe City. Jpn. J. Water Treat. Biol., 24, 19-24 (in Japanese with English title).
Strain(s): 35 

Konno, R., Wakabayashi, A. 1987 Effects of chemical substance on the growth of algae [Sôrui no zôsyoku ni oyobosu kagakubusshitsu no eikyô]. Tokyo-to Kankyô Kagaku Kenkyûjo Nenpô. 1987, 4 pp. (in Japanese).
Strain(s): 35 

Yagi, O., Hagiwara, T., Takamura, Y., Sudo, R. 1987 Limiting nutrients of algal growth in Lake Kasumigaura. Jpn. J. Water Poll. Res., 10, 115-122 (in Japanese with English summary).
Keywords: Microcystis; blue-green algae; nutrients; algal growth potential
Strain(s): 35478 

Yagi, O., Sudo, R., Imaoka, T., Teranishi, S. 1984 Algal growth potential on munisipal wastewater using red tide organisms. Res. Rep. Natl. Inst. Environ. Stud., No. 63, 231-242 (in Japanese with English summary).
Strain(s): 35 

Sudo, R., Tai, S., Yagi, O., Okada, M., Hosomi, M., Yamane, A. N. 1981 Comprehensive studies on the eutrophication of fresh-water areas. Determination of algal growth potential by algal assay procedure. Res. Rep. Natl. Inst. Environ. Stud., No. 26, 1-53 (in Japanese with English title).
Strain(s): 35 

Hosomi, M., Sudo, R. 1979 Studies on the effects of sediments on algal growth - Algal growth potential of sediments. Res. Rep. Natl. Inst. Environ. Stud., No. 6, 115-121 (in Japanese with English summary).
Strain(s): 35 

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