Strain number NIES-2199  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Botryococcus braunii Kützing  
Former name  
Common name Green alga  
Locality (Date of collection) brick pits, Madingley, Cambridge, England, U.K.  
Latitude / Longitude 52.223633 / 0.040854 
Habitat (Isolation source)  
History < IAM (2007) < Hara, Yoshiaki < UTEX (1986)  
Isolator (Date of isolation) Droop, M. R.  
Identified by  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Jan]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  3-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information 18S rRNA ( AB780365 )  
Cell size (min - max) 8 - 15 μm  
Characteristics oil (hydrocarbon) production (Race A)  
Other strain no. Other collection strain no. : IAM C-529; CCAP 807/1; UTEX LB572; SAG B 807-1
Remarks Cryopreserved[2017 Nov]; Axenic; Very slow growth 
Zhu, J. & Wakisaka, M. 2019 Effect of air nanobubble water on the growth and metabolism of Haematococcus lacustris and Botryococcus braunii. J. Nutr. Sci. Vitaminol., 65, S212-S216.
Keywords: Botryococcus braunii; Haematococcus lacustris; astaxanthin; lipid; nanobubble water
Strain(s): 1442199 
PubMed: 31619633
DOI: 10.3177/jnsv.65.S212

Ghosh, R., Makam, R., Krishnamurthy, V. 2018 Effect of critical parameters on biomass yield from Botryococcus braunii by Response surface methodology. Chem. Sci. Rev. Lett., 7, 409-420.
Keywords: Bio mass; Botryococcus Braunii; Microalgae; Response; Surface Methodology
Strain(s): 2199 

Huang, Y-T., Lai, C-W., Wu, B-W., Lin, K-S., Wu, J.C.S., Md Shahriar A Hossain, Yamauchi, Y., Wu, C.-W. 2017 Advances in bioconversion of microalgae with high biomass and lipid productivity. J. Taiwan Inst. Chem. Eng., 79, 37-42.
Keywords: Bioconversion; Fatty acid; methyl esters; Illumination; Microalgae
Strain(s): 2199 
DOI: 10.1016/j.jtice.2017.05.026

Nogami, R., Ushijima, K., Nishida, H., Wakisaka, M. 2017 Enhancement of growth and lipid production of Botryococcus braunii by steel slags. J. Jpn. Inst. Energy, 96, 372-375 (in Japanese with English summary).
Keywords: Botryococcus braunii; Blast furnace slag; Growth promotion; Lipid production; Steelmaking slag
Strain(s): 2199 
DOI: 10.3775/jie.96.372

Hamana, K., Niitsu, M., Hayashi, H. 2013 Occurrence of homospermidine and thermospermine as a cellular polyamine in unicellular chlorophyte and multicellular charophyte green algae J. Gen. Appl. Micobiol., 59, 313-319.
Keywords: green alga; homospermidine; polyamine; thermospermine
Strain(s): 1815116025529630636036364072283614091410141114191837216421993374 
PubMed: 24005181
DOI: 10.2323/jgam.59.313

Huang, Y., Lee, H., Lai, C. 2013 Engineering of the growth environment of microalgae with high biomass and lipid productivity. J. Nanosci. Nanotechnol., 13, 2117-2121.
Keywords: Microalgae; Cultivation Environment; Carbon Dioxide; Biomass Productivity; Lipid Productivity
Strain(s): 2199 
DOI: 10.1166/jnn.2013.6877

Honda, R., Boonnorat, J., Chiemchaisri, C., Chiemchaisri, w., Yamamoto, K. 2012 Carbon dioxide capture and nutrients removal utilizing treated sewage by concentrated microalgae cultivation in a membrane photobioreactor. Bioresour. Technol., 125, 59-64.
Keywords: Carbon dioxide capture; Nutrients removal; Membrane photobioreactor; Microalgae; Botryococcus braunii
Strain(s): 2199 
PubMed: 23023237
DOI: 10.1016/j.biortech.2012.08.138

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