Strain number NIES-2172  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella vulgaris Beijerinck  
Former name Chlorella ovalis Butcher (before 1993)  
Common name Green alga  
Locality (Date of collection) U.K.  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM < CCAP (Butcher, R. W.);  
Isolator (Date of isolation) George, E. A.  
Identified by Kessler, E.; Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488581 )  
Cell size (min - max)  
Organization Unicellular 
Other strain no. Other collection strain no. : IAM C-176; CCAP 211/21A or B (?)
Remarks Cryopreserved; Axenic 
Jiang, M., Nakano, S. 2022 The crucial influence of trophic status on the relative requirement of nitrogen to phosphorus for phytoplankton growth. Water Res., 222, 118868 (article ID).
Keywords: Nitrogen; Phosphorus; Trophic status; Phytoplankton; Nitrogen-to-phosphorus ratio; Limiting factor
Strain(s): 4420932172 
DOI: 10.1016/j.watres.2022.118868

Jiang, M., Nakano, S. 2021 Application of image analysis for algal biomass quantifcation: a low‑cost and non‑destructive method based on HSI color space. J. Appl. Phycol., 33, 3709–3717.
Keywords: Algal biomass; Quantitative methods; Image analysis; HSI color space; Lambert–Beer law
Strain(s): 3332172 
DOI: 10.1007/s10811-021-02571-4

Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K. 2018 Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent. Biotechnol. Biofuels, 11, 176 (article ID).
Keywords: Microalgae; Wastewater efuent; Enhanced biomass production; Microalgae growth-promoting bacteria
Strain(s): 4821722235 
PubMed: 29983739
DOI: 10.1186/s13068-018-1174-0

Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429.
Keywords: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine
Strain(s): 217222352848284928502851 
PubMed: 19796121
DOI: 10.1111/j.1365-2672.2009.04542.x

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