Strain number NIES-2172  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella vulgaris Beijerinck  
Synonym  
Former name Chlorella ovalis Butcher (before 1993)  
Common name Green alga  
Locality (Date of collection) U.K.  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM < CCAP (Butcher, R. W.);  
Isolator (Date of isolation) George, E. A.  
Identified by Kessler, E.; Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488581 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics  
Other strain no. Other collection strain no. : IAM C-176; CCAP 211/21A or B (?)
 
Remarks Cryopreserved; Axenic 
Movie  
Reference
Jiang, M., Nakano, S. 2022 The crucial influence of trophic status on the relative requirement of nitrogen to phosphorus for phytoplankton growth. Water Res., 222, 118868 (article ID).
Keywords: Nitrogen; Phosphorus; Trophic status; Phytoplankton; Nitrogen-to-phosphorus ratio; Limiting factor
Strain(s): 4420932172 
DOI: 10.1016/j.watres.2022.118868

Jiang, M., Nakano, S. 2021 Application of image analysis for algal biomass quantifcation: a low‑cost and non‑destructive method based on HSI color space. J. Appl. Phycol., 33, 3709–3717.
Keywords: Algal biomass; Quantitative methods; Image analysis; HSI color space; Lambert–Beer law
Strain(s): 3332172 
DOI: 10.1007/s10811-021-02571-4

Toyama, T., Kasuya, M., Hanaoka, T., Kobayashi, N., Tanaka, Y., Inoue, D., Sei, K., Morikawa, M., Mori, K. 2018 Growth promotion of three microalgae, Chlamydomonas reinhardtii, Chlorella vulgaris and Euglena gracilis, by in situ indigenous bacteria in wastewater efuent. Biotechnol. Biofuels, 11, 176 (article ID).
Keywords: Microalgae; Wastewater efuent; Enhanced biomass production; Microalgae growth-promoting bacteria
Strain(s): 4821722235 
PubMed: 29983739
DOI: 10.1186/s13068-018-1174-0

Fujii, K., Nakashima, H., Hashidzume, Y. 2010 Isolation of folate-producing microalgae, from oligotrophic ponds in Yamaguchi, Japan. J. Appl. Microbiol., 108, 1421-1429.
Keywords: B-vitamins; Chlamydomonas; Chlorococcum; folate; microalgae; thiamine
Strain(s): 217222352848284928502851 
PubMed: 19796121
DOI: 10.1111/j.1365-2672.2009.04542.x

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