Strain number NIES-2171  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Micractinium inermum Hoshina & Fujiwara  
Former name Chlorella sp.; Chlorella vulgaris Beijerinck  
Common name Green alga  
Locality (Date of collection) Botanical garden, Sendai, Sendai, Miyagi, Japan  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Murano, Fumio  
Isolator (Date of isolation) Murano, Fumio  
Identified by Hoshina, Ryo (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2020 Oct]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488574 ) , 18S-ITS1-5.8S-ITS2 ( JX889641 ) , 18S-ITS1-5.8S-ITS2-28S ( AB731604 )  
Cell size (min - max) 2 - 7 μm  
Characteristics Authentic strain (Hoshina et al. 2013)  
Other strain no. Other collection strain no. : IAM C-135
Other strain no. : Kiyohara U-140  
Remarks Cryopreserved; Axenic 
Pröschold, T., Pitsch, G., Darienko, T. 2020 Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a new endosymbiont isolated from ciliates. Diversity-Basel, 12, 200 (article ID).
Keywords: Micractinium tetrahymenae; Tetrahymena; Utricularia; facultative endosymbiosis; ciliate-algae symbiosis
Strain(s): 216721714033 
DOI: 10.3390/d12050200

Pröschold, T., Darienko, T. 2020 Choricystis and Lewiniosphaera gen. nov. (Trebouxiophyceae Chlorophyta), two different green algal endosymbionts in freshwater sponges. Symbiosis, 82, 175-188.
Keywords: Choricystis; Lewiniosphaera; Freshwater sponges; Endosymbiosis
Strain(s): 21672171234240334292 
PubMed: 33328698
DOI: 10.1007/s13199-020-00711-x

Hoshina, R., Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167217121732330 
DOI: 10.1111/pre.12010

Higashiyama, T., Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195.
Strain(s): 2163216621712189 
PubMed: 1956777
DOI: 10.1093/nar/19.22.6191

Yamada, T., Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

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