Strain number NIES-2168  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella sorokiniana Shihira & R.W.Krauss  
Former name Chlorella vulgaris Beijerinck  
Common name Green alga  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Murano, Fumio  
Isolator (Date of isolation) Murano, Fumio  
Identified by Fukushima, Hiroshi; Kessler, E.; Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2020 Oct]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488572 )  
Cell size (min - max)  
Organization Unicellular 
Other strain no. Other collection strain no. : IAM C-133
Other strain no. : Murano (407)U-145  
Remarks Cryopreserved; Axenic 
Liu, X., Fujiwara, M., Kodera, T., Watanabe, K., Akizuki, S., Kishi, M., Koyama, M., Toda, T., Ban, S. 2020 Conditions for continuous cultivation of Chlorella sorokiniana and nutrient removal from anaerobic digestion effluent of aquatic macrophytes. Int. Biodeterior. Biodegrad., 149, 104923 (article ID).
Keywords: Flow-through cultivation; pH-controlled process; Biomass recovery; Green algae; Energy recycling; Wastewater treatment
Strain(s): 2168 
DOI: 10.1016/j.ibiod.2020.104923

Azaman, S. N. A., Wong, D. C. J., Tan, S. W., Yusoff, F. M., Nagao, N., Yeap, S. K. 2020 De novo transcriptome analysis of Chlorella sorokiniana: effect of glucose assimilation, and moderate light intensity. Sci Rep, 10, 17331 (article ID).
Strain(s): 2168 
PubMed: 33060668
DOI: 10.1038/s41598-020-74410-4

Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary).
Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution
Strain(s): 39214521682170225622572258 
DOI: 10.2965/jswe.42.239

Kawamura, K., Sumii, K., Matsumoto, M., Nakase, D., Kosaki, Y., Ishikawa, M. 2018 Determining the optimal cultivation strategy for microalgae for biodiesel production using flow cytometric monitoring and mathematical modeling. Biomass Bioenerg., 117, 24-31.
Keywords: Biofuel; Logistic equation; Optimal cultivation; Simulation model; Triacylglycerols
Strain(s): 2168 
DOI: 10.1016/j.biombioe.2018.07.005

Wang, Y., Chiu, SY., Ho, SH., Liu, Z., Hasunuma, T., Chang, TT., Chang, KF., Chang, JS., Ren NQ., Kondo, A. 2016 Improving carbohydrate production of Chlorella sorokiniana NIES-2168 through semi-continuous process coupled with mixotrophic cultivation. Biotechnol. J., 11, 1072–1081.
Keywords: Carbohydrate; Chlorella; Fed-batch; Mixotrophic cultivation; Semi-continuous process
Strain(s): 2168 
PubMed: 27312599
DOI: 10.1002/biot.201500270

Kishi, M., Kawai, M., Toda, T. 2015 Heterotrophic utilization of ethylene glycol and propylene glycol by Chlorella protothecoides. Algal Res., 11, 428-434.
Keywords: algae; Chlorella protothecoides; Ethylene glycol; Propylene glycol; Heterotrophic; pH
Strain(s): 215121682170 
DOI: 10.1016/j.algal.2015.04.020

Eio, E. J., Kawai, M., Niwa, C., Ito, M., Yamamoto, S., Toda, T. 2015 Biodegradation of bisphenol A by an algal-bacterial system. Environ. Sci. Pollut. Res., 22, 15145–15153.
Keywords: Algal-bacterial system; Bacterial consortium; Biodegradation; Bisphenol A; Inhibition; Photosynthetic oxygen
Strain(s): 2168 
PubMed: 26013738
DOI: 10.1007/s11356-015-4693-2

Dionisio-Sese, M. L., Miyachi, S. 1992 The effect of sodium chloride on carbonic anhydrase activity in marine microalgae. J. Phycol., 28, 619-624.
Keywords: carbonic anhydrase; Cl- activation; Cl- inhibition; marine microalgae; NaCl effect; photosynthesis
Strain(s): 2168 
DOI: 10.1111/j.0022-3646.1992.00619.x

Ohta, S., Chang, T., Aozasa, O., Kondo, M., Miyata, H. 1992 Sustained production of arachidonic and eicosapentaenoic acids by the red alga Porphyridium purpureum cultured in a light/dark cycle. J. Ferment. Bioengin., 74, 398-402.
Strain(s): 214021682256 
DOI: 10.1016/0922-338X(92)90040-2

Yamada, T., Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.