| Strain number | NIES-2167 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Trebouxiophyceae | |||
| Scientific name | Didymogenes sphaerica Fujiwara | |||
| Synonym | ||||
| Former name | Chlorella sorokiniana Shihira et Krauss; Chlorella vulgaris Beijerinck | |||
| Common name | Green alga | |||
| Locality (Date of collection) | ||||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | ||||
| History | < IAM (2007) < Yakult < IAM < Watanabe, Atsushi | |||
| Isolator (Date of isolation) | Watanabe, Atsushi | |||
| Identified by | Hoshina, Ryo (Reidentify) | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb] | |||
|
Culture condition (Preculture condition) |
Medium:
C (agar)
Temperature: 20 C Light intensity: 8-15 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 3 M |
|||
| Gene information | 18S rRNA ( AB488789 ) , 18S-ITS1-5.8S-ITS2-28S ( AB731603 ) | |||
| Cell size (min - max) | 3 - 10 μm | |||
| Organization | ||||
| Characteristics | Authentic strain (Hoshina et al. 2013) | |||
| Other strain no. |
Other collection strain no. : IAM C-43
|
|||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
|---|
|
Pröschold, T., Pitsch, G., Darienko, T.
2020
Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a new endosymbiont isolated from ciliates.
Diversity-Basel,
12,
200 (article ID).
Keywords: Micractinium tetrahymenae; Tetrahymena; Utricularia; facultative endosymbiosis; ciliate-algae symbiosis Strain(s): 2167, 2171, 4033 DOI: 10.3390/d12050200 Krivina, E. S., Temraleeva, A. D. 2020 Identification problems and cryptic diversity of Chlorella-clade microalgae (Chlorophyta). Microbiology, 89, 720-732. Keywords: microalgae; morphology; ecology; phylogeny; 18S−ITS1−5.8S−ITS2 fragments; polyphasic approach Strain(s): 2167, 2173, 4032, 4034 DOI: 10.1134/S0026261720060107 Hoshina, R., Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132. Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept Strain(s): 2167, 2171, 2173, 2330 DOI: 10.1111/pre.12010 |
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