Strain number NIES-2167  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Didymogenes sphaerica Fujiwara  
Former name Chlorella sorokiniana Shihira et Krauss; Chlorella vulgaris Beijerinck  
Common name Green alga  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (2007) < Yakult < IAM < Watanabe, Atsushi  
Isolator (Date of isolation) Watanabe, Atsushi  
Identified by Hoshina, Ryo (Reidentify)  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488789 ) , 18S-ITS1-5.8S-ITS2-28S ( AB731603 )  
Cell size (min - max) 3 - 10 μm  
Characteristics Authentic strain (Hoshina et al. 2013)  
Other strain no. Other collection strain no. : IAM C-43
Remarks Cryopreserved; Axenic 
Pröschold, T., Pitsch, G., Darienko, T. 2020 Micractinium tetrahymenae (Trebouxiophyceae, Chlorophyta), a new endosymbiont isolated from ciliates. Diversity-Basel, 12, 200 (article ID).
Keywords: Micractinium tetrahymenae; Tetrahymena; Utricularia; facultative endosymbiosis; ciliate-algae symbiosis
Strain(s): 216721714033 
DOI: 10.3390/d12050200

Krivina, E. S., Temraleeva, A. D. 2020 Identification problems and cryptic diversity of Chlorella-clade microalgae (Chlorophyta). Microbiology, 89, 720-732.
Keywords: microalgae; morphology; ecology; phylogeny; 18S−ITS1−5.8S−ITS2 fragments; polyphasic approach
Strain(s): 2167217340324034 
DOI: 10.1134/S0026261720060107

Hoshina, R., Fujiwara, Y. 2013 Molecular characterization of Chlorella cultures of the National Institute for Environmental Studies culture collection with description of Micractinium inermum sp. nov., Didymogenes sphaerica sp. nov., Didymogenes soliella sp. nov., (Chlorellaceae, Trebouxiophyceae). Phycol. Res., 61, 124-132.
Keywords: Chlorella; Didymogenes; group 1 intron; internal transcribed spacer 2; Micractinium; species concept
Strain(s): 2167217121732330 
DOI: 10.1111/pre.12010

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