Strain number NIES-2166  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Coccomyxa subellipsoidea E.Acton  
Former name Chlorella vulgaris Beijerinck; 'Chlorella' saccharophila (Krüger) Migula  
Common name Green alga  
Locality (Date of collection) Marble Point Antarctica  
Latitude / Longitude  
Habitat (Isolation source) (Dried algal peat)  
History < IAM (2007) < Holm-Hansen, O.  
Isolator (Date of isolation) Holm-Hansen, O.  
Identified by Kessler, E.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information Whole-genome ( AGSI00000000 )  
Cell size (min - max) 3 - 10 μm  
Organization Unicellular 
Characteristics Genome decoded strain (Blanc et al. 2012)  
Other strain no. Other collection strain no. : IAM C-169
Other strain no. : Holm-Hansen M-42-a  
Remarks Cryopreserved; Axenic 
Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., Kulikovskiy, M. 2021 A new species of freshwater algae Nephrochlamys yushanlensis sp. nov. (Selenastraceae, Sphaeropleales) and its lipid accumulation during nitrogen and phosphorus starvation. J. Phycol., 57, 606-618.
Strain(s): 2166 
PubMed: 33296071
DOI: 10.1111/jpy.13116

Kania, K., Zienkiewicz, M., Drożak, A. 2020 Stable transformation of unicellular green alga Coccomyxa subellipsoidea C-169 via electroporation. Protoplasma, 257, 607–611.
Keywords: Coccomyxa subellipsoidea C-169; Hygromycin B resistance; Electrotransformation; Electroporation; Stable genome transformation
Strain(s): 2166 
PubMed: 31741062
DOI: 10.1007/s00709-019-01447-2

Hashizume, M., Yoshida, M., Demura, M., Watanabe, M. M. 2020 Culture study on utilization of phosphite by green microalgae. J. Appl. Phycol., 32, 889–899.
Keywords: Phosphite utilization; Chlorella vulgaris; Coccomyxa subellipsoidea
Strain(s): 216621702280 
DOI: 10.1007/s10811-020-02088-2

Maltsev, Y., Maltseva, I., Maltseva, S., Kociolek, J. P., Kulikovskiy, M. 2019 Fatty acid content and profile of the novel strain of Coccomyxa elongata (Trebouxiophyceae, Chlorophyta) cultivated at reduced nitrogen and phosphorusconcentrations. J. Phycol., 55, 1154-1165.
Keywords: Coccomyxa; fatty acid profile lipid; nitrogen and phosphorus deprivation; Trebouxiophyceae
Strain(s): 216622522353 
PubMed: 31318981
DOI: 10.1111/jpy.12903

Hamada, M., Schröder, K., Bathia, J., Kürn, U., Fraune, S., Khalturina, M., Khalturin, K., Shinzato, C., Satoh, N., Bosch, T. C.G. 2018 Metabolic co-dependence drives the evolutionary ancient Hydra-Chlorella symbiosis. eLife, 7, e35122 (article ID).
Keywords: Chlorella; Hydra; evolutionary biology; genome; nitrogen metabolism; symbiosis
Strain(s): 216622352541 
PubMed: 29848439
DOI: 10.7554/eLife.35122

Ranjan, P., Kateriya, S. 2018 Localization and dimer stability of a newly identifed microbial rhodopsin from apolar, non-motile green algae. BMC Res. Notes, 11, 65 (article ID).
Keywords: Coccomyxa subellipsoidea; Proton-pumping rhodopsin; Optogenetics; Localization of the microbial rhodopsin in eukaryotes; Chlorella vulgaris
Strain(s): 2166 
PubMed: 29361974
DOI: 10.1186/s13104-018-3181-4

Zongyi, Y., Lu, L., Chen, J., Wei, D. 2018 Effect of crude glycerol on heterotrophic growth of Chlorella pyrenoidosa and Coccomyxa subellipsoidea C-169. J. Appl. Phycol., 30, 2989–2996.
Keywords: Chlorophyceae; Microalgae; Crude glycerol; Heterotrophic growth; Biodiesel
Strain(s): 2166 
DOI: 10.1007/s10811-018-1551-x

Bertuzz, S., Gustavs, L., Pandolfini, G., Tretiac, M. 2017 Heat shock treatments for the control of lithobionts: A case study with epilithic green microalgae. Int. Biodeterior. Biodegrad., 123, 236-243.
Keywords: Biocides; Biodeteriogens; Chlorophyll a fluorescence; Hydration; Polyols; Non-reducing sugars
Strain(s): 2166 
DOI: 10.1016/j.ibiod.2017.06.023

Wang, C., Wang, Z., Luo, F., Li, Y. 2017 The augmented lipid productivity in an emerging oleaginous model alga Coccomyxa subellipsoidea by nitrogen manipulation strategy. World J. Microbiol. Biotechnol., 33, 160 (article ID).
Keywords: Coccomyxa subellipsoidea; Nitrogen manipulation; Lipid; Fatty acids; Metabolites
Strain(s): 2166 
PubMed: 28752265
DOI: 10.1007/s11274-017-2324-4

Peng, H., Wei, D., Chen, G., Chen, F. 2016 Transcriptome analysis reveals global regulation in response to CO2 supplementation in oleaginous microalga Coccomyxa subellipsoidea C-169. Biotechnol. Biofuels, 9, 151 (article ID).
Keywords: Coccomyxa subellipsoidea C-169; Elevated CO2; Lipid accumulation; Transcriptomic analysis; Phosphoenolpyruvate carboxylase; Pyruvate carboxylase; Carbamoyl-phosphate synthetase II; Ferredoxin; Vacuolar; H+-ATPase
Strain(s): 2166 
PubMed: 27453726
DOI: 10.1186/s13068-016-0571-5

Pfaff, S., Borchhardt, N., Boy, J., Karsten, Ulf., Gustavs, L. 2016 Desiccation tolerance and growth-temperature requirements of Coccomyxa (Trebouxiophyceae, Chlorophyta) strains from Antarctic biological soil crusts. Algol. Stud., 151/152, 3-19.
Keywords: Antarctica; biological soil crusts; ecophysiology; desiccation; cold adaptation; endemis
Strain(s): 2166 
DOI: 10.1127/algol_stud/2016/0245

Lemieux, C., Otis, C.,Turmel, M. 2014 Chloroplast phylogenomic analysis resolves deep-level relationships within the green algal class Trebouxiophyceae. BMC Evol. Biol., 14, 211 (article ID).
Keywords: Chlorophyta; Trebouxiophyceae; Plastid genome; Phylogenomics
Strain(s): 18242166 
PubMed: 25270575
DOI: 10.1186/s12862-014-0211-2

Blanc, G., Agarkova, I., Grimwood, J., Kou, A., Brueggeman, A., Dunigan, D. D., Gurnon, J., Ladunga, I., Lindquist, E., Lucas, S., Pangilinan, J., Proschold, T., Salamov, A., Schmutz, J., Weeks, D., Yamada, T., Lomsadze, A., Borodovsky, M., Claverie, J. M., Grigoriev, I. V., Van Etten, J. L. 2012 The genome of the polar eukaryotic microalga Coccomyxa subellipsoidea reveals traits of cold adaptation. Genome Biol., 13, R39 (article ID).
Strain(s): 2166 
PubMed: 22630137
DOI: 10.1186/gb-2012-13-5-r39

Smith, D. R., Burki, F., Yamada, T., Grimwood, J., Grigoriev, I. V., Van Etten, J. L., Keeling, P. J. 2011 The GC-rich mitochondrial and plastid genomes of the green alga Coccomyxa give insight into the evolution of organelle DNA nucleotide landscape. PLoS One, 6, e23624 (article ID).
Strain(s): 2166 
PubMed: 21887287
DOI: 10.1371/journal.pone.0023624

Higashiyama, T., Yamada, T. 1991 Electrophoretic karyotyping and chromosomal gene mapping of Chlorella. Nucleic Acids Res., 19, 6191-6195.
Strain(s): 2163216621712189 
PubMed: 1956777
DOI: 10.1093/nar/19.22.6191

Yamada, T., Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

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