Strain number NIES-2162  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Parachlorella kessleri (Fott & Nováková) Krienitz, E.H.Hegewald, Hepperle, V.Huss, T.Rohr & M.Wolf  
Synonym Chlorella kessleri Fott & Nováková 1969  
Former name Formerly identified as Chlorella vulgaris, re-identified at NIES by DNA analysis  
Common name Green alga  
Locality (Date of collection) New York City, New York, USA (locality of wild type strain (NIES-2160))  
Latitude / Longitude 40.703546 /  
Habitat (Isolation source) Freshwater  
History < IAM (2007) < Kamiya, A. (1990-10-23) < Miyachi, Shigetoh < Schmid, G. H.  
Isolator (Date of isolation)  
Identified by Re-identified by NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488599 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics White mutant of NIES-2160  
Other strain no. Other collection strain no. : IAM C-539; SAG 9.80
Other strain no. : Schwarze 125  
Remarks Cryopreserved; Axenic 
Kamiya, A. 1989 Effects of blue light and ammonia on nitrogen metabolism in a colorless mutant of Chlorella. Plant Cell Physiol., 30, 513-521.
Keywords: ammonia effect; blue light effect; Chlorella; glutamine synthetase; nitrate uptake; nitrogen metabolism
Strain(s): 2162 
DOI: 10.1093/oxfordjournals.pcp.a077770

Kamiya, A. 1988 Blue light-induced in vivo absorbance changes and in vitro activation of nitrate reductase in a nitrate-starved Chlorella mutant. Plant Cell Physiol., 29, 489-496.
Keywords: blue light effect; Chlorella; light-induced absorbancechange; NADH-nitrate reductase
Strain(s): 2162 
DOI: 10.1093/oxfordjournals.pcp.a077519

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 6872160216121622170217322352280 
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Miyachi, S., Kamiya, A., Muto, S. 1977 Isolation of peroxisomes from colorless Chlorella mutant cells and intracellular localization of isocitratelyase, malate synthase and phosphoenolpyruvate carboxylase. Plant Cell Physiol., Spec. Iss. (Photosynthetic Organelles), 347-353.
Strain(s): 2162 

Miyachi, S., Kamiya, S., Miyachi, S. 1977 Wavelength-effect of incident light on carbon metabolish in Chlorella cells. In Biological Solar Energy Conversion, Eds. by Mitsui, A. et al., Academic Press, New York, pp. 167-182.
Strain(s): 21602162 

Kamiya, A., Miyachi, S. 1975 Blue light-induced formation of photphoenolpyruvate carboxylase in colorless Chlorella mutant cells. Plant Cell Physiol., 16, 729-736.
Strain(s): 2162 

Kamiya, A., Miyachi, S. 1974 Effects of blue light on respiration and carbon dioxyde fixation in colorless Chlorella mutant cells. Plant Cell Physiol., 15, 927-937.
Strain(s): 2162 
DOI: 10.1093/oxfordjournals.pcp.a075082

Schwarze, P., Frandsen, N. O. 1960 Herstellung von Chlorella-Farbmutanten mit Hilfe von radioaktiven Isotopen. Naturwissenschaften, 47, 47.
Strain(s): 21612162 

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