Strain Data

Strain number		NIES-2160
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Parachlorella kessleri (Fott & Nováková) Krienitz, E.H.Hegewald, Hepperle, V.Huss, T.Rohr & M.Wolf
Synonym		Chlorella kessleri Fott & Nováková 1969
Former name
Common name		Green alga
Locality (Date of collection)		New York City, New York, USA
Latitude / Longitude		40.703546 /
Habitat (Isolation source)		Freshwater
History		< IAM (2007) < Tsuzuki, Mikio (1988) < Miyachi, Shigetoh (1977) < Schmid, G. H.
Isolator (Date of isolation)		Pratt, R.
Identified by		Confirmed by DNA sequencing at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		18S rRNA ( AB488597 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Starch production (Nakamura et al. 1982)
Other strain no.		Other collection strain no. : IAM C-531; ATCC 11468; CCAP 211/11H; SAG 211-11h; UTEX 263
Remarks		Cryopreserved; Axenic
Movie

Reference
Miyauchi, H., Harada, K., Suzuki, Y., Okada, K., Aoki, M., Umemura, T., Fujiwara,S., Tsuzuki, M. 2021 Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production. Algal Res., 58, 102394 (article ID). Keywords: Microalgae; Attached cell culture; Wastewater; Phosphorus uptake; Chlorella Strain(s): 2160 DOI: 10.1016/j.algal.2021.102394 Strain(s): 24, 47, 144, 2146, 2160 Miyauchi, H., Okada, K., Fujiwara, S., Tsuzuki, M. 2020 Characterization of CO₂ fixation on algal biofilms with an infrared gas analyzer and importance of a space-rich structure on the surface. Algal Res., 46, 101814 (article ID). Keywords: Attached culture; Carbon fixation; Chlorella; Photosynthesis Strain(s): 2160 DOI: 10.1016/j.algal.2020.101814 Tsuzuki, M., Okada, K., Isoda, H., Hirano, M., Odaka, T., Saijo, H., Aruga, R., Miyauchi, H., Fujiwara, S. 2019 Physiological properties of photoautotrophic microalgae and cyanobacteria relevant to industrial biomass production. Mar. Biotechnol., 21, 406-415. Keywords: Algae; Biomass; Chlorella; Culture system; Cyanobacteria; Photosynthesis Strain(s): 2160 PubMed: 30927152 DOI: 10.1007/s10126-019-09890-1 Tamamizu, K. & Kumazaki, S. 2019 Spectral microscopic imaging of heterocysts and vegetative cells in two filamentous cyanobacteria based on spontaneous Raman scattering and photoluminescence by 976 nm excitation. Biochim. Biophys. Acta-Bioenerg., 1860, 78-88. Keywords: Heterocystous cyanobacteria; Raman scattering microscopy; Anabaena variabilis; Rivularia; Photosystems; Thylakoid membranes Strain(s): 2095, 2160 PubMed: 30414930 DOI: 10.1016/j.bbabio.2018.11.012 Hasegawa, M., Yoshida, T., Yabuta, M., Terazima, M., Kumazaki, S. 2011 Anti-Stokes fluorescence spectra of chloroplasts in Parachlorella kessleri and maize at room temperature as characterized by near-infrared continuous-wave laser fluorescence microscopy and absorption microscopy. J. Phys. Chem. B, 115, 4184-4194. Strain(s): 2160 PubMed: 21417246 DOI: 10.1021/jp111306k Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116. Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection Strain(s): 18, 144, 250, 530, 551, 2138, 2160, 2258, 2280 PubMed: 18079144 DOI: 10.1093/pcp/pcm172 Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184. Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection Strain(s): 2160, 2169, 2173, 2186, 4016 DOI: 10.1046/j.1529-8817.2003.02052. Yamamoto, M., Nozaki, H., Kawano, S. 2001 Evolutionary relationships among multiple modes of cell division in the genus Nannochloris (Chlorophyta) revealed by genome size, actin gene multiplicity, and phylogeny. J. Phycol., 37, 106-120. Keywords: actin gene; autosporulation; binary fission; budding; cell division; Chlorophyta; DNA content; genome size; phylogeny; Nannochloris Strain(s): 2160, 4016 DOI: 10.1046/j.1529-8817.2001.037001106.x Furihata, T., Pomprasirt, S., Sakurai, H. 1997 Characteristics of sulfite transport by Chlorella vulgaris. Plant Cell Physiol., 38, 398-403. Keywords: Chlorella; plasma membrane; sulfite; transport Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a029182 Takeda, H. 1993 Chemical composition of cell walls as a taxonomical marker. J. Plant Res., 106, 195-200. Keywords: anisotropy; cell wall sugar composition; chemotaxonomy; Chlorella; classification; lytic enzyme; rigid wall; ruthenium red Strain(s): 2160, 2170 DOI: 10.1007/BF02344585 Shiraiwa, Y., Goyal, A., Tolbert, N. E. 1993 Alkalization of the medium by unicellular green algae during uptake of dissolved inorganic carbon. Plant Cell Physiol., 34, 649-657. Keywords: alkalization; dissolved inorganic carbon concentrating mechanism; photosynthesis Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a078467 Shiraiwa, Y. & Miyachi, S. 1993 Factors controlling induction of carbonic anhydrase and efficiency of photosynthesis in Chlorella vulgaris 11h cells. Plant Cell Physiol., 24, 919-923. Keywords: carbonic anhydrase; Chlorella vulgaris (11h cell); CO2 fixation; photosynthesis Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a076596 Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553. Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176 DOI: 10.1111/j.0022-3646.1992.00550.x Takeda, H. 1991 Sugar composition of the cell wall and the taxonomy of Chlorella (Chlorophyceae). J. Phycol., 27, 224-232. Keywords: anisotropy; cell wall sugar composition; chemotaxonomy; Chlorella; Chlorophyta; classification; glucosamine; rigid wall; ruthenium red staining Strain(s): 2160, 2170 DOI: 10.1111/j.0022-3646.1991.00224.x Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO₂ concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856. Strain(s): 48, 2093, 2138, 2160, 2235 PubMed: 1666759 DOI: 10.1104/pp.93.3.851 Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104. Strain(s): 2093, 2138, 2160, 2173, 2235 DOI: 10.1016/0304-3770(89)90051-X Grotjohann, N. & Kowallik, W. 1989 Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri. Physiol. Plant., 75, 43-46. Keywords: blue/UV-photoreceptor; Chlorella kessleri; phosphofructokinase Strain(s): 2160 DOI: 10.1111/j.1399-3054.1989.tb02061.x Takeda, H. 1988 Classification of Chlorella strains by cell wall sugar composition. Phytochemistry, 27, 3823-3826. Keywords: Chlorella ellipsoidea; C. fusca; C. kessleri; C. saccharophila; C. sorokiniana; C. vulgaris; Chlorophyta; anisotropy; cell wall sugar composition; chemotaxonomy; Ruthenium Red staining Strain(s): 226, 2150, 2160, 2170 DOI: 10.1016/0031-9422(88)83025-5 Miyachi, S. & Miyachi, S. 1987 Some biochemical changes related to starch breakdown induced by blue light illumination and by addition of ammonia to Chlorella cells. Plant Cell Physiol., 28, 309-314. Keywords: ammonia; adenylates; blue light; Chlorella; orthophosphate; phosphorylase Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a077297 Kuchitsu, K., Oh-hama, T., Tsuzuki, M., Miyachi, S. 1987 Detection and characterization of acidic compartments (vacuoles) in Chlorella vulgaris 11h cells by ³¹P-in vivo NMR spectroscopy and cytochemical techniques. Arch. Mikrobiol., 148, 83-87. Strain(s): 2160 Hirano, H., Takeda, Y., Iwamura, T. 1986 Unique repetitive sequences of 170 bp in Chlorella. Plant Mol. Biol., 7, 311-317. Keywords: Chlorella; HaeIII family; repetitive sequence; tandem repeat Strain(s): 2160, 2170 PubMed: 24302372 DOI: 10.1007/BF00752903 Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233. Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport Strain(s): 2093, 2160, 2173, 2235 DOI: 10.1111/j.1574-6968.1986.tb01860.x Tsuzuki, M. 1986 Transport and fixation of inorganic carbon in the cells of Chlorella vulgaris 11h grown under ordinary air. Plant Cell Physiol., 27, 1233-1240. Keywords: carbon dioxide; carbonic anhydrase; Chlorella vulgaris; inorganic carbon; photosynthesis Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a077220 Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319. Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus Strain(s): 687, 2157, 2160, 2170, 2173, 2280 Tsuzuki, M., Miyachi, S., Berry, J. A. 1985 Intercellular accumulation of inortanic carbon and its active species taken up by Chlorella vulgaris 11h. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 53-66. Strain(s): 2160 Nakamura, Y. & Imamura, M. 1985 Regulation of ADP-glucose pyrophosphorylase from Chlorella vulgaris. Plant Physiol., 78, 601-605. Strain(s): 2160 PubMed: 16664290 DOI: 10.1104/pp.78.3.601 Miyachi, S. & Miyachi, S. 1985 Ammonia induces starch degradation in Chlorella cells. Plant Cell Physiol., 26, 245-252. Keywords: ammonia effect; Chlorella; dark CO2 fixation; starch degradation; sucrose formation Strain(s): 687, 2157, 2160, 2170, 2173 DOI: 10.1093/oxfordjournals.pcp.a076903 Shiraiwa, Y. & Miyachi, S. 1985 Effects of temperature and CO₂ concentration on induction of carbonic anhydrase and changes in efficiency of photosynthesis in Chlorella vulgaris 11h. Plant Cell Physiol., 26, 543-549. Keywords: carbonic anhydrase; Chlorella vulgaris; CO2 concentration; photosynthesis; temperature Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a076938 Miyachi, S., Tsuzuki, M., Yagawa, Y. 1985 Carbonic anhydrase in various microalgae. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 145-154. Strain(s): 2093, 2160, 2173 Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO₂ concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C₃-plants. II. Electron Transport, Transport of CO₂, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92. Strain(s): 2160, 2235 Ohmori, M., Miyachi, S., Okabe, K., Miyachi, S. 1984 Effects of ammonia on respiration, adenylate levels, amino acid synthesis and CO₂ fixation in cells of Chlorella vulgaris 11h in darkness. Plant Cell Physiol., 25, 749-756. Keywords: adenylate level; amino acid; ammonia effect; Chlorella vulgaris; dark CO2 fixation; respiration Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a076768 Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240. Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280 DOI: 10.1111/j.1749-6632.1984.tb12341.x Nakamura, Y. & Imamura, M. 1983 Change in properties of starch when photosynthesized at different temperature in Chlorella vulgaris. Plant Sci. Lett., 31, 123-131. Keywords: Chlorella; photosynthetic CO2 fixation; starch metabolism; temperature Strain(s): 2160 DOI: 10.1016/0304-4211(83)90048-2 Nakamura, Y. 1983 Change in molecular weight distribution in starch when degraded at different temperatures in Chlorella vulgaris. Plant Sci. Lett., 30, 259-265. Keywords: Chlorella; photosynthetic CO2 fixation; starch metabolism; temperature Strain(s): 2160 DOI: 10.1016/0304-4211(83)90164-5 Nakamura, Y. & Imamura, M. 1983 Characterization of Chlorella phosphorylase: the glucan specificity and effect of temperature. Phytochemistry, 22, 2395-2399. Keywords: Chlorella vulgaris; Chlorophyceae; phosphorylase; photosynthesis; starch; temperature Strain(s): 2160 DOI: 10.1016/0031-9422(83)80126-5 Tsuzuki, M. 1983 Mode of HCO₃-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37. Strain(s): 2160, 2170, 2235 Miyachi, S., Tsuzuki, M., Avramova, S. T. 1983 Utilization modes of inorganic carbon for photosynthesis in various species of Chlorella. Plant Cell Physiol., 24, 441-451. Keywords: bicarbonate fixation; carbonic anhydrase; Chlorella; photosynthetic CO2-fixation Strain(s): 687, 2093, 2157, 2160, 2173 Nakamura, Y. & Imamura, M. 1983 Characteristics of a-glucan phosphorylase from Chlorella vulgaris. Phytochemistry, 22, 835-840. Keywords: Chlorella vulgaris; Chlorophyceae; phospholyrase; starch; ADP-glucose; UDP-glucose Strain(s): 2160 DOI: 10.1016/0031-9422(83)85008-0 Nakamura, Y. & Miyachi, S. 1982 Effect of temperature on starch degradation in Chlorella vulgaris 11h cells. Plant Cell Physiol., 23, 333-341. Keywords: Chlorella vulgaris; CO2 fixation; starch; sucrose; temperature (starch degradation) Strain(s): 2160 Matumura, H., Muto, S., Miyachi, S. 1982 Light-induced conversion of NAD⁺ to NADP⁺ in Chlorella cells. Biochim. Biophys. Acta, 679, 300-307. Strain(s): 2160, 2161 DOI: 10.1016/0005-2728(82)90300-0 Nakamura, Y. & Miyachi, S. 1982 Changes in starch photosynthesized at different temperature in Chlorella. Plant Sci. Lett., 27, 1-6. Strain(s): 2160 DOI: 10.1016/0304-4211(82)90065-7 Tsuzuki, M., Muto, S., Miyachi, S. 1981 Role of carbonic anhydrase in photosynthesis of higher plants: A possible limiting step in CO₂ transport. In Photosynthesis. VI. Regulation of Carbon Metabolism, Ed. by Akoyunoglou, G., Balaban International Science Services, Philadelphia, pp. 483-492. Strain(s): 2160 Yamada, T. & Sakaguchi, K. 1981 Protoplast induction in Chlorella species. Agric. Biol. Chem., 45, 1905-1909. Strain(s): 685, 2160, 2165, 2170, 2189 DOI: 10.1271/bbb1961.45.1905 Shiraiwa, Y., Fakler, J., Miyachi, S. 1981 Factors controlling carbonic anhydrase activity in Chlorella vulgalis 11h. In Photosynthesis. VI. Regulation of Carbon Metabolism, Ed. by Akoyunoglou, G., Balaban International Science Services, Philadelphia, pp. 493-499. Strain(s): 2160 Miyachi, S. & Tsuzuki, M. 1981 Carbonic anhydrase and carbon uptake in photosynthesis of green algae and terrestrial C₃ plants. In Proceedings of the First Binational Joint-Symposium under Japan-Australia Cooperative Science Programme "Integration of Photosynthetic Carbon Metabolism as Basis of Plant Productivity", pp. 15-18. Strain(s): 2160 Nakamura, Y. & Miyachi, S. 1980 Effects of temperature on glycolate metabolism in Chlorella. Plant Cell Physiol., 21, 1541-1549. Keywords: Chlorella; CO2 fixation; glucose-polymer; glycolate; sucrose; temperature Strain(s): 2160 DOI: 10.1093/pcp/21.8.1541 Nakamura, Y. & Miyachi, S. 1980 Effects of temperature and CO₂ concentration on photosynthetic CO₂ fixation by Chlorella. Plant Cell Physiol., 21, 765-774. Keywords: Chlorella; CO2 fixation; glucose-polymer; glycolate; sucrose; temperature Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a076051 Tsuzuki, M., Shiraiwa, Y., Miyachi, S. 1980 Role of carbonic anhydrase in photosynthesis in Chlorella derived from kinetic analysis of ¹⁴CO₂ fixation. Plant Cell Physiol., 21, 677-688. Keywords: carbonic anhydrase; Chlorella; CO2 fixation; kinetic analysis; photosynthesis Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a076042 Miyachi, S. & Miyachi, S. 1980 Effects of ammonia on carbon metabolism in photosynthesizing Chlorella vulgaris 11h: the replacement of blue light by ammonium ion. In The Blue Light Syndrome, Ed. by Senger, H., Springer-Verlag, Berlin, pp. 429-434. Strain(s): 2160 Miyachi, S., Kamiya, A., Miyachi, S. 1980 Effects of blue light on respiration and non-photosynthetic CO₂ fixation in Chlorella 11 h cells. In The Blue Light Syndrome, Ed. by Senger, H., Springer-Verlag, Berlin, pp. 321-328. Strain(s): 2160 Hogetsu, D. & Miyachi, S. 1979 Role of carbonic anhydrase in photosynthetic CO₂ fixation in Chlorella. Plant Cell Physiol., 20, 747-756. Keywords: carbonic anhydrase; Chlorella; CO2 fixation; photosynthesis; ribulose 1,5-bisphosphate carboxylase Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a075865 Tsuzuki, M. & Miyachi, S. 1979 Effects of CO₂ concentration during growth and of ethoxyzolamide on CO₂ compensation point in Chlorella. FEBS Lett., 103, 221-223. Strain(s): 2160 Miyachi, S. & Shiraiwa, Y. 1979 Form of inorganic carbon utilized for photosynthesis in Chlorella vulgaris 11h cells. Plant Cell Physiol., 20, 341-348. Keywords: carbon dioxide fixation; carbonic anbydrase; Chlorella; photosynthesis; transport of CO2 Strain(s): 2160 DOI: 10.1093/oxfordjournals.pcp.a075818 Hogetsu, D. & Miyachi, S. 1977 Effects of CO₂ concentration during growth on subsequent photosynthetic CO₂ fixation. Plant Cell Physiol., 18, 347-352. Strain(s): 2160 Nakamura, Y. & iyachi, S. 1977 Conversion of glucose-polymer to sucrose in Chlorella induced by high temperature. In Biological Solar Energy Conversion, Eds. by Mitsui, A. et al., Academic Press, New York, pp. 203-212. Strain(s): 2160 Miyachi, S., Hogetsu, D., Miyachi, S. 1977 Effect of environmental factors on photosynthetic carbon metabolism. In Photosynthetic CO2 Assimilation and Photorespiration, Eds. by Vaklinova, S. G. et al., Publishing House of Bulgarian Academy of Science, Sofia, pp. 15-30. Strain(s): 2160 Miyachi, S., Kamiya, S., Miyachi, S. 1977 Wavelength-effect of incident light on carbon metabolish in Chlorella cells. In Biological Solar Energy Conversion, Eds. by Mitsui, A. et al., Academic Press, New York, pp. 167-182. Strain(s): 2160, 2162

Reference

Miyauchi, H., Harada, K., Suzuki, Y., Okada, K., Aoki, M., Umemura, T., Fujiwara,S., Tsuzuki, M. 2021 Development of an algal cell-attached solid surface culture system for simultaneous wastewater treatment and biomass production. Algal Res., 58, 102394 (article ID).
Keywords: Microalgae; Attached cell culture; Wastewater; Phosphorus uptake; Chlorella
Strain(s): 2160
DOI: 10.1016/j.algal.2021.102394

Strain(s): 24, 47, 144, 2146, 2160

Miyauchi, H., Okada, K., Fujiwara, S., Tsuzuki, M. 2020 Characterization of CO₂ fixation on algal biofilms with an infrared gas analyzer and importance of a space-rich structure on the surface. Algal Res., 46, 101814 (article ID).
Keywords: Attached culture; Carbon fixation; Chlorella; Photosynthesis
Strain(s): 2160
DOI: 10.1016/j.algal.2020.101814

Tsuzuki, M., Okada, K., Isoda, H., Hirano, M., Odaka, T., Saijo, H., Aruga, R., Miyauchi, H., Fujiwara, S. 2019 Physiological properties of photoautotrophic microalgae and cyanobacteria relevant to industrial biomass production. Mar. Biotechnol., 21, 406-415.
Keywords: Algae; Biomass; Chlorella; Culture system; Cyanobacteria; Photosynthesis
Strain(s): 2160
PubMed: 30927152
DOI: 10.1007/s10126-019-09890-1

Tamamizu, K. & Kumazaki, S. 2019 Spectral microscopic imaging of heterocysts and vegetative cells in two filamentous cyanobacteria based on spontaneous Raman scattering and photoluminescence by 976 nm excitation. Biochim. Biophys. Acta-Bioenerg., 1860, 78-88.
Keywords: Heterocystous cyanobacteria; Raman scattering microscopy; Anabaena variabilis; Rivularia; Photosystems; Thylakoid membranes
Strain(s): 2095, 2160
PubMed: 30414930
DOI: 10.1016/j.bbabio.2018.11.012

Hasegawa, M., Yoshida, T., Yabuta, M., Terazima, M., Kumazaki, S. 2011 Anti-Stokes fluorescence spectra of chloroplasts in Parachlorella kessleri and maize at room temperature as characterized by near-infrared continuous-wave laser fluorescence microscopy and absorption microscopy. J. Phys. Chem. B, 115, 4184-4194.
Strain(s): 2160
PubMed: 21417246
DOI: 10.1021/jp111306k

Shimonaga, T., Konishi, M., Oyama, Y., Fujiwara, S., Satoh, A., Fujita, N., Colleoni, C., Buléon, A., Putaux, J.-L., Ball, S. G., Yokoyma, A., Hara, Y., Nakamura, Y., Tsuzuki, M. 2008 Variation in Storage α-polyglucans of the Porphyridiales (Rhodophyta). Plant Cell Physiol., 49, 103-116.
Keywords: Amylose; Floridean starch; Glycogen; Porphyridiales; Rhodophyta; Semi-amylopection
Strain(s): 18, 144, 250, 530, 551, 2138, 2160, 2258, 2280
PubMed: 18079144
DOI: 10.1093/pcp/pcm172

Yamamoto, M., Nozaki, H., Miyazawa, Y., Koide, T., Kawano, S. 2003 Relationship between presence of a mother cell wall and speciation in the unicellular microalga Nannochloris (Chlorophyta). J. Phycol., 39, 172-184.
Keywords: 18S rRNA; actin gene; autosporulation; binary fission; budding; Chlorella; Chlorophyta; molecular phylogeny; TBR, tree bisection reconnection
Strain(s): 2160, 2169, 2173, 2186, 4016
DOI: 10.1046/j.1529-8817.2003.02052.

Yamamoto, M., Nozaki, H., Kawano, S. 2001 Evolutionary relationships among multiple modes of cell division in the genus Nannochloris (Chlorophyta) revealed by genome size, actin gene multiplicity, and phylogeny. J. Phycol., 37, 106-120.
Keywords: actin gene; autosporulation; binary fission; budding; cell division; Chlorophyta; DNA content; genome size; phylogeny; Nannochloris
Strain(s): 2160, 4016
DOI: 10.1046/j.1529-8817.2001.037001106.x

Furihata, T., Pomprasirt, S., Sakurai, H. 1997 Characteristics of sulfite transport by Chlorella vulgaris. Plant Cell Physiol., 38, 398-403.
Keywords: Chlorella; plasma membrane; sulfite; transport
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a029182

Takeda, H. 1993 Chemical composition of cell walls as a taxonomical marker. J. Plant Res., 106, 195-200.
Keywords: anisotropy; cell wall sugar composition; chemotaxonomy; Chlorella; classification; lytic enzyme; rigid wall; ruthenium red
Strain(s): 2160, 2170
DOI: 10.1007/BF02344585

Shiraiwa, Y., Goyal, A., Tolbert, N. E. 1993 Alkalization of the medium by unicellular green algae during uptake of dissolved inorganic carbon. Plant Cell Physiol., 34, 649-657.
Keywords: alkalization; dissolved inorganic carbon concentrating mechanism; photosynthesis
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a078467

Shiraiwa, Y. & Miyachi, S. 1993 Factors controlling induction of carbonic anhydrase and efficiency of photosynthesis in Chlorella vulgaris 11h cells. Plant Cell Physiol., 24, 919-923.
Keywords: carbonic anhydrase; Chlorella vulgaris (11h cell); CO2 fixation; photosynthesis
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a076596

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 686, 687, 2150, 2151, 2160, 2163, 2169, 2175, 2176
DOI: 10.1111/j.0022-3646.1992.00550.x

Takeda, H. 1991 Sugar composition of the cell wall and the taxonomy of Chlorella (Chlorophyceae). J. Phycol., 27, 224-232.
Keywords: anisotropy; cell wall sugar composition; chemotaxonomy; Chlorella; Chlorophyta; classification; glucosamine; rigid wall; ruthenium red staining
Strain(s): 2160, 2170
DOI: 10.1111/j.0022-3646.1991.00224.x

Tsuzuki, M., Ohnuma, E., Sato, N., Takaku, T., Kawaguchi, A. 1990 Effects of CO₂ concentration during growth on fatty acid composition in microalgae. Plant Physiol., 93, 851-856.
Strain(s): 48, 2093, 2138, 2160, 2235
PubMed: 1666759
DOI: 10.1104/pp.93.3.851

Tsuzuki, M. & Miyachi, S. 1989 The function of carbonic anhydrase in aquatic photosynthesis. Aquatic Botany, 34, 85-104.
Strain(s): 2093, 2138, 2160, 2173, 2235
DOI: 10.1016/0304-3770(89)90051-X

Grotjohann, N. & Kowallik, W. 1989 Influence of blue light on the activity of phosphofructokinase in Chlorella kessleri. Physiol. Plant., 75, 43-46.
Keywords: blue/UV-photoreceptor; Chlorella kessleri; phosphofructokinase
Strain(s): 2160
DOI: 10.1111/j.1399-3054.1989.tb02061.x

Takeda, H. 1988 Classification of Chlorella strains by cell wall sugar composition. Phytochemistry, 27, 3823-3826.
Keywords: Chlorella ellipsoidea; C. fusca; C. kessleri; C. saccharophila; C. sorokiniana; C. vulgaris; Chlorophyta; anisotropy; cell wall sugar composition; chemotaxonomy; Ruthenium Red staining
Strain(s): 226, 2150, 2160, 2170
DOI: 10.1016/0031-9422(88)83025-5

Miyachi, S. & Miyachi, S. 1987 Some biochemical changes related to starch breakdown induced by blue light illumination and by addition of ammonia to Chlorella cells. Plant Cell Physiol., 28, 309-314.
Keywords: ammonia; adenylates; blue light; Chlorella; orthophosphate; phosphorylase
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a077297

Kuchitsu, K., Oh-hama, T., Tsuzuki, M., Miyachi, S. 1987 Detection and characterization of acidic compartments (vacuoles) in Chlorella vulgaris 11h cells by ³¹P-in vivo NMR spectroscopy and cytochemical techniques. Arch. Mikrobiol., 148, 83-87.
Strain(s): 2160

Hirano, H., Takeda, Y., Iwamura, T. 1986 Unique repetitive sequences of 170 bp in Chlorella. Plant Mol. Biol., 7, 311-317.
Keywords: Chlorella; HaeIII family; repetitive sequence; tandem repeat
Strain(s): 2160, 2170
PubMed: 24302372
DOI: 10.1007/BF00752903

Aizawa, K. & Miyachi, S. 1986 Carbonic anhydrase and CO₂ concentrating mechanisms in microalgae and cyanobacteria. FEMS Microbiol. Lett., 39, 215-233.
Keywords: aquatic algae; cyanobacteria; photoautotrophs; photorespiration; photosynthesis; transport
Strain(s): 2093, 2160, 2173, 2235
DOI: 10.1111/j.1574-6968.1986.tb01860.x

Tsuzuki, M. 1986 Transport and fixation of inorganic carbon in the cells of Chlorella vulgaris 11h grown under ordinary air. Plant Cell Physiol., 27, 1233-1240.
Keywords: carbon dioxide; carbonic anhydrase; Chlorella vulgaris; inorganic carbon; photosynthesis
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a077220

Miyachi, S., Tsuzuki, M., Maruyama, I., Ganter, M., Miyachi, S. 1986 Effects of CO₂ concentration during growth on the intracellular structure of Chlorella and Scenedesmus (Chlorophyta). J. Phycol., 22, 313-319.
Keywords: Chlorella; CO2 concentration; electron microscopy; pyrenoid starch; Scenedesmus
Strain(s): 687, 2157, 2160, 2170, 2173, 2280

Tsuzuki, M., Miyachi, S., Berry, J. A. 1985 Intercellular accumulation of inortanic carbon and its active species taken up by Chlorella vulgaris 11h. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 53-66.
Strain(s): 2160

Nakamura, Y. & Imamura, M. 1985 Regulation of ADP-glucose pyrophosphorylase from Chlorella vulgaris. Plant Physiol., 78, 601-605.
Strain(s): 2160
PubMed: 16664290
DOI: 10.1104/pp.78.3.601

Miyachi, S. & Miyachi, S. 1985 Ammonia induces starch degradation in Chlorella cells. Plant Cell Physiol., 26, 245-252.
Keywords: ammonia effect; Chlorella; dark CO2 fixation; starch degradation; sucrose formation
Strain(s): 687, 2157, 2160, 2170, 2173
DOI: 10.1093/oxfordjournals.pcp.a076903

Shiraiwa, Y. & Miyachi, S. 1985 Effects of temperature and CO₂ concentration on induction of carbonic anhydrase and changes in efficiency of photosynthesis in Chlorella vulgaris 11h. Plant Cell Physiol., 26, 543-549.
Keywords: carbonic anhydrase; Chlorella vulgaris; CO2 concentration; photosynthesis; temperature
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a076938

Miyachi, S., Tsuzuki, M., Yagawa, Y. 1985 Carbonic anhydrase in various microalgae. In Inorganic Carbon Uptake by Aquatic Photosynthetic Organisms, Eds. by Lucas, W. J. & Berry, J. A., American Society of Plant Physiologists, Rockville, Md, pp. 145-154.
Strain(s): 2093, 2160, 2173

Miyachi, S., Tsuzuki, M., Aizawa, K. 1985 Factors which enhance photosynthesis in microalgae under limiting CO₂ concentrations. In Kinetics of Photosynthetic Carbon Metabolism in C₃-plants. II. Electron Transport, Transport of CO₂, Mathematical Models, Eds. by Viil, J. et al., Akademiya Nauka, Estonia SSR, pp. 88-92.
Strain(s): 2160, 2235

Ohmori, M., Miyachi, S., Okabe, K., Miyachi, S. 1984 Effects of ammonia on respiration, adenylate levels, amino acid synthesis and CO₂ fixation in cells of Chlorella vulgaris 11h in darkness. Plant Cell Physiol., 25, 749-756.
Keywords: adenylate level; amino acid; ammonia effect; Chlorella vulgaris; dark CO2 fixation; respiration
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a076768

Tsuzuki, M., Shimamoto, T., Yang, S. Y., Miyachi, S. 1984 Diversity in intracellular locality, nature, and function of carbonic anhydrase in verious plants. Ann. NY Acad. Sci., 429, 238-240.
Strain(s): 687, 2160, 2161, 2162, 2170, 2173, 2235, 2280
DOI: 10.1111/j.1749-6632.1984.tb12341.x

Nakamura, Y. & Imamura, M. 1983 Change in properties of starch when photosynthesized at different temperature in Chlorella vulgaris. Plant Sci. Lett., 31, 123-131.
Keywords: Chlorella; photosynthetic CO2 fixation; starch metabolism; temperature
Strain(s): 2160
DOI: 10.1016/0304-4211(83)90048-2

Nakamura, Y. 1983 Change in molecular weight distribution in starch when degraded at different temperatures in Chlorella vulgaris. Plant Sci. Lett., 30, 259-265.
Keywords: Chlorella; photosynthetic CO2 fixation; starch metabolism; temperature
Strain(s): 2160
DOI: 10.1016/0304-4211(83)90164-5

Nakamura, Y. & Imamura, M. 1983 Characterization of Chlorella phosphorylase: the glucan specificity and effect of temperature. Phytochemistry, 22, 2395-2399.
Keywords: Chlorella vulgaris; Chlorophyceae; phosphorylase; photosynthesis; starch; temperature
Strain(s): 2160
DOI: 10.1016/0031-9422(83)80126-5

Tsuzuki, M. 1983 Mode of HCO₃-utilization by the cells of Chlamydomonas reinhardtii grown under ordinary air. Z. Pflanzenphysiol., 110, 29-37.
Strain(s): 2160, 2170, 2235

Miyachi, S., Tsuzuki, M., Avramova, S. T. 1983 Utilization modes of inorganic carbon for photosynthesis in various species of Chlorella. Plant Cell Physiol., 24, 441-451.
Keywords: bicarbonate fixation; carbonic anhydrase; Chlorella; photosynthetic CO2-fixation
Strain(s): 687, 2093, 2157, 2160, 2173

Nakamura, Y. & Imamura, M. 1983 Characteristics of a-glucan phosphorylase from Chlorella vulgaris. Phytochemistry, 22, 835-840.
Keywords: Chlorella vulgaris; Chlorophyceae; phospholyrase; starch; ADP-glucose; UDP-glucose
Strain(s): 2160
DOI: 10.1016/0031-9422(83)85008-0

Nakamura, Y. & Miyachi, S. 1982 Effect of temperature on starch degradation in Chlorella vulgaris 11h cells. Plant Cell Physiol., 23, 333-341.
Keywords: Chlorella vulgaris; CO2 fixation; starch; sucrose; temperature (starch degradation)
Strain(s): 2160

Matumura, H., Muto, S., Miyachi, S. 1982 Light-induced conversion of NAD⁺ to NADP⁺ in Chlorella cells. Biochim. Biophys. Acta, 679, 300-307.
Strain(s): 2160, 2161
DOI: 10.1016/0005-2728(82)90300-0

Nakamura, Y. & Miyachi, S. 1982 Changes in starch photosynthesized at different temperature in Chlorella. Plant Sci. Lett., 27, 1-6.
Strain(s): 2160
DOI: 10.1016/0304-4211(82)90065-7

Tsuzuki, M., Muto, S., Miyachi, S. 1981 Role of carbonic anhydrase in photosynthesis of higher plants: A possible limiting step in CO₂ transport. In Photosynthesis. VI. Regulation of Carbon Metabolism, Ed. by Akoyunoglou, G., Balaban International Science Services, Philadelphia, pp. 483-492.
Strain(s): 2160

Yamada, T. & Sakaguchi, K. 1981 Protoplast induction in Chlorella species. Agric. Biol. Chem., 45, 1905-1909.
Strain(s): 685, 2160, 2165, 2170, 2189
DOI: 10.1271/bbb1961.45.1905

Shiraiwa, Y., Fakler, J., Miyachi, S. 1981 Factors controlling carbonic anhydrase activity in Chlorella vulgalis 11h. In Photosynthesis. VI. Regulation of Carbon Metabolism, Ed. by Akoyunoglou, G., Balaban International Science Services, Philadelphia, pp. 493-499.
Strain(s): 2160

Miyachi, S. & Tsuzuki, M. 1981 Carbonic anhydrase and carbon uptake in photosynthesis of green algae and terrestrial C₃ plants. In Proceedings of the First Binational Joint-Symposium under Japan-Australia Cooperative Science Programme "Integration of Photosynthetic Carbon Metabolism as Basis of Plant Productivity", pp. 15-18.
Strain(s): 2160

Nakamura, Y. & Miyachi, S. 1980 Effects of temperature on glycolate metabolism in Chlorella. Plant Cell Physiol., 21, 1541-1549.
Keywords: Chlorella; CO2 fixation; glucose-polymer; glycolate; sucrose; temperature
Strain(s): 2160
DOI: 10.1093/pcp/21.8.1541

Nakamura, Y. & Miyachi, S. 1980 Effects of temperature and CO₂ concentration on photosynthetic CO₂ fixation by Chlorella. Plant Cell Physiol., 21, 765-774.
Keywords: Chlorella; CO2 fixation; glucose-polymer; glycolate; sucrose; temperature
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a076051

Tsuzuki, M., Shiraiwa, Y., Miyachi, S. 1980 Role of carbonic anhydrase in photosynthesis in Chlorella derived from kinetic analysis of ¹⁴CO₂ fixation. Plant Cell Physiol., 21, 677-688.
Keywords: carbonic anhydrase; Chlorella; CO2 fixation; kinetic analysis; photosynthesis
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a076042

Miyachi, S. & Miyachi, S. 1980 Effects of ammonia on carbon metabolism in photosynthesizing Chlorella vulgaris 11h: the replacement of blue light by ammonium ion. In The Blue Light Syndrome, Ed. by Senger, H., Springer-Verlag, Berlin, pp. 429-434.
Strain(s): 2160

Miyachi, S., Kamiya, A., Miyachi, S. 1980 Effects of blue light on respiration and non-photosynthetic CO₂ fixation in Chlorella 11 h cells. In The Blue Light Syndrome, Ed. by Senger, H., Springer-Verlag, Berlin, pp. 321-328.
Strain(s): 2160

Hogetsu, D. & Miyachi, S. 1979 Role of carbonic anhydrase in photosynthetic CO₂ fixation in Chlorella. Plant Cell Physiol., 20, 747-756.
Keywords: carbonic anhydrase; Chlorella; CO2 fixation; photosynthesis; ribulose 1,5-bisphosphate carboxylase
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a075865

Tsuzuki, M. & Miyachi, S. 1979 Effects of CO₂ concentration during growth and of ethoxyzolamide on CO₂ compensation point in Chlorella. FEBS Lett., 103, 221-223.
Strain(s): 2160

Miyachi, S. & Shiraiwa, Y. 1979 Form of inorganic carbon utilized for photosynthesis in Chlorella vulgaris 11h cells. Plant Cell Physiol., 20, 341-348.
Keywords: carbon dioxide fixation; carbonic anbydrase; Chlorella; photosynthesis; transport of CO2
Strain(s): 2160
DOI: 10.1093/oxfordjournals.pcp.a075818

Hogetsu, D. & Miyachi, S. 1977 Effects of CO₂ concentration during growth on subsequent photosynthetic CO₂ fixation. Plant Cell Physiol., 18, 347-352.
Strain(s): 2160

Nakamura, Y. & iyachi, S. 1977 Conversion of glucose-polymer to sucrose in Chlorella induced by high temperature. In Biological Solar Energy Conversion, Eds. by Mitsui, A. et al., Academic Press, New York, pp. 203-212.
Strain(s): 2160

Miyachi, S., Hogetsu, D., Miyachi, S. 1977 Effect of environmental factors on photosynthetic carbon metabolism. In Photosynthetic CO2 Assimilation and Photorespiration, Eds. by Vaklinova, S. G. et al., Publishing House of Bulgarian Academy of Science, Sofia, pp. 15-30.
Strain(s): 2160

Miyachi, S., Kamiya, S., Miyachi, S. 1977 Wavelength-effect of incident light on carbon metabolish in Chlorella cells. In Biological Solar Energy Conversion, Eds. by Mitsui, A. et al., Academic Press, New York, pp. 167-182.
Strain(s): 2160, 2162

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