Strain number NIES-2159  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Parachlorella kessleri (Fott & Nováková) Krienitz, E.H.Hegewald, Hepperle, V.Huss, T.Rohr & M.Wolf  
Synonym Chlorella kessleri Fott & Nováková 1969  
Former name  
Common name Green alga  
Locality (Date of collection) New York City, New York, USA  
Latitude / Longitude 40.703546 /  
Habitat (Isolation source) Freshwater  
History < IAM (2007) < Soeder, C. J. (1966) < CCAP  
Isolator (Date of isolation) Winokur (1945-**-**)  
Identified by Kessler, E. (1993); Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 16S rRNA ( AJ242769 AJ387750 ) , 18S rRNA ( AJ242765 AB488596 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Starch production (Takeshita et al. 2014)  
Other strain no. Other collection strain no. : IAM C-208; CAUP H1901; CCAP 211/11G; SAG 211-11g; UTEX 262
Remarks Cryopreserved; Axenic 
Noguchi, M., Aizawa, R., Nakazawa, D., Hakumura, Y., Furuhashi, Y., Yang, S., Ninomiya, K.,Takahashi, K., Honda, R. 2021 Application of real treated wastewater to starch production by microalgae: Potential effect of nutrients and microbial contamination. Biochem. Eng. J., 169, 107973 (article ID).
Keywords: Biorefinery; Chlorella vulgaris; Chlorination; Microbial contamination; Municipal wastewater; Starch (carbohydrate) production
Strain(s): 215121592170 
DOI: 10.1016/j.bej.2021.107973

Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID).
Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod
Strain(s): 21512152215921692170 
PubMed: 34681157
DOI: 10.3390/biology10101060

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 215121522159216921702179 
PubMed: 24583913
DOI: 10.1016/j.biortech.2014.01.135

Chu, C. Y., Liao, W. R., Huang, R., Lin, L. P. 2004 Haemagglutinating and antibiotic activities of freshwater microalgae. World J. Microbiol. Biotechnol., 20, 817–825.
Keywords: Antibiotic activity; erythrocytes; freshwater microalgae; haemagglutination
Strain(s): 22622721592163216521692175218922082211221322142229223122332240224122432247224832773284 
DOI: 10.1007/s11274-004-8712-6

Yamada, T. & Sakaguchi, K. 1982 Comparative studies on Chlorella cell walls: Induction of protoplast formation. Arch. Mikrobiol., 132, 10-13.
Strain(s): 2276862159216321662168217121892352 
DOI: 10.1007/BF00690809

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