| Strain number | NIES-2152 | |||
|---|---|---|---|---|
| Phylum | Chlorophyta | |||
| Class | Trebouxiophyceae | |||
| Scientific name | Parachlorella kessleri (Fott & Nováková) Krienitz, Hegewald, Hepperle, Huss, Rohr & Wolf | |||
| Synonym | Chlorella kessleri Fott & Nováková | |||
| Former name | ||||
| Common name | Green alga | |||
| Locality (Date of collection) | ||||
| Latitude / Longitude | ||||
| Habitat (Isolation source) | ||||
| History | < IAM (2007) < Yakult < IAM; | |||
| Isolator (Date of isolation) | Watanabe, Atsushi | |||
| Identified by | Kessler, E. (1994); Confirmed at NIES by DNA sequencing | |||
| State of strain | Cryopreservation; Unialgal; Clonal; Axenic[2008 Apr] | |||
|
Culture condition (Preculture condition) |
Medium:
C (agar)
Temperature: 20 C Light intensity: 8-15 µmol photons/m2/sec, L/D cycle: 10L:14D Duration: 3 M |
|||
| Gene information | Whole-genome ( BBXU00000000 ) , 18S rRNA ( AB488589 ) | |||
| Cell size (min - max) | ||||
| Organization | Unicellular | |||
| Characteristics | Starch production (Takeshita et al. 2014) ; Genome decoded strain (Ota et al. 2016) | |||
| Other strain no. |
Other collection strain no. : IAM C-33
|
|||
| Remarks | Cryopreserved; Axenic | |||
| Movie | ||||
| Reference |
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Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N.
2019
Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion.
Carbohydr. Polym.,
208/,
142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion Strain(s): 94, 227, 2152, 2173, 2195, 2280 PubMed: 30658785 DOI: 10.1016/j.carbpol.2018.12.057 Otaki, R., Oishi, Y., Abe, S., Fujiwara, S., Sato, N. 2019 Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri. Bioresour. Technol., 289, 121686 (article ID). Keywords: Carbon metabolism; Chlorella kessleri; Gene expression; Seawater-based biofuel; Triacylglycero Strain(s): 2152 PubMed: 31238290 DOI: 10.1016/j.biortech.2019.121686 Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišová,K., Zachleder, V., Kawano, S. 2018 Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation. Algal Res., 35, 416-426. Keywords: Parachlorella kessleri; Nutrient dilution; 150-L mass cultivation; Whole genome sequencing; Mutation identification; Single-nucleotide polymorphism Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.005 Yamazaki, T., Konosu, E., Takeshita, T., Hireta, A., Ota, S., Kazama, Y., Abe, T., Kawano, S. 2018 Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Res., 36, 37-47. Keywords: Chlorella; Lipids; Starch; Sulfur assimilation; Sulfur starvation Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.022 Hayashia, T., Otakia, R., Hiraia, K., Tsuzukia, M., Sato, N. 2017 Optimization of seawater-based triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, through simultaneous imposition of loweredtemperature and enhanced-light intensity. Algal Res., 28, 100-107. Keywords: Chlorella kessleri; Freshwater green algae; Low temperature; Moderate light; Seawater; Triacylglycerol Strain(s): 2152 DOI: 10.1016/j.algal.2017.10.016 Ota, S. & Kawano, S. 2017 Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella. Bio-protocol, 7 Keywords: Alga; Chlorella; Parachlorella; Phosphorus; Polyphosphate; Molybdenum blue reaction Strain(s): 2152 PubMed: 27180903 DOI: 10.21769/BioProtoc.2539 Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID). Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM Strain(s): 2152 PubMed: 26811738 DOI: 10.1186/s13068-016-0424-2 Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID). Strain(s): 2152 PubMed: 27180903 DOI: 10.1038/srep25731 Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134. Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light Strain(s): 2151, 2152, 2159, 2169, 2170, 2179 DOI: 10.1016/j.biortech.2014.01.135 Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187. Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri Strain(s): 2152 PubMed: 24997835 Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Bioresour. Technol., 149, 432-438. Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation Strain(s): 2152 PubMed: 24135567 DOI: 10.1016/j.biortech.2013.09.079 |
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