Strain Data

Strain number		NIES-2152
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Parachlorella kessleri (Fott & Nováková) Krienitz, Hegewald, Hepperle, Huss, Rohr & Wolf
Synonym		Chlorella kessleri Fott & Nováková
Former name
Common name		Green alga
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Yakult < IAM;
Isolator (Date of isolation)		Watanabe, Atsushi
Identified by		Kessler, E. (1994); Confirmed by DNA sequencing at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2008 Apr]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		Whole-genome ( BBXU00000000 ) , 18S rRNA ( AB488589 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Starch production (Takeshita et al. 2014) ; Genome decoded strain (Ota et al. 2016)
Other strain no.		Other collection strain no. : IAM C-33
Remarks		Cryopreserved; Axenic
Movie

Reference
Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151. Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion Strain(s): 94, 227, 2152, 2173, 2195, 2280 PubMed: 30658785 DOI: 10.1016/j.carbpol.2018.12.057 Otaki, R., Oishi, Y., Abe, S., Fujiwara, S., Sato, N. 2019 Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri. Bioresour. Technol., 289, 121686 (article ID). Keywords: Carbon metabolism; Chlorella kessleri; Gene expression; Seawater-based biofuel; Triacylglycero Strain(s): 2152 PubMed: 31238290 DOI: 10.1016/j.biortech.2019.121686 Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2019 The Parachlorella genome and transcriptome endorse active RWP-RK, meiosis and flagellar genes in trebouxiophycean algae. Cytologia, 84, 323-330. Keywords: Evolution; Flagellum; MID; Meiosis; Parachlorella; RWP-RK Strain(s): 2152 DOI: 10.1508/cytologia.84.323 Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišová,K., Zachleder, V., Kawano, S. 2018 Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation. Algal Res., 35, 416-426. Keywords: Parachlorella kessleri; Nutrient dilution; 150-L mass cultivation; Whole genome sequencing; Mutation identification; Single-nucleotide polymorphism Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.005 Yamazaki, T., Konosu, E., Takeshita, T., Hireta, A., Ota, S., Kazama, Y., Abe, T., Kawano, S. 2018 Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Res., 36, 37-47. Keywords: Chlorella; Lipids; Starch; Sulfur assimilation; Sulfur starvation Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.022 Hayashia, T., Otakia, R., Hiraia, K., Tsuzukia, M., Sato, N. 2017 Optimization of seawater-based triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, through simultaneous imposition of loweredtemperature and enhanced-light intensity. Algal Res., 28, 100-107. Keywords: Chlorella kessleri; Freshwater green algae; Low temperature; Moderate light; Seawater; Triacylglycerol Strain(s): 2152 DOI: 10.1016/j.algal.2017.10.016 Ota, S., Kawano, S. 2017 Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella. Bio-protocol, 7, e2539 (article ID). Keywords: Alga; Chlorella; Parachlorella; Phosphorus; Polyphosphate; Molybdenum blue reaction Strain(s): 2152 DOI: 10.21769/BioProtoc.2539 Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID). Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM Strain(s): 2152 PubMed: 26811738 DOI: 10.1186/s13068-016-0424-2 Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID). Strain(s): 2152 PubMed: 27180903 DOI: 10.1038/srep25731 Takeshita, T., Takeda,K., Ota, S., Yamazaki, T., Kawano, S. 2015 A simple method for measuring the starch and lipid contents in the cell of microalgae. Cytologia, 80, 475-481. Keywords: Chlorella; Starch; Lipid; Microplate; Trade-off Strain(s): 2152 DOI: 10.1508/cytologia.80.475 Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134. Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light Strain(s): 2151, 2152, 2159, 2169, 2170, 2179 PubMed: 24583913 DOI: 10.1016/j.biortech.2014.01.135 Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187. Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri Strain(s): 2152 PubMed: 24997835 Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Bioresour. Technol., 149, 432-438. Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation Strain(s): 2152 PubMed: 24135567 DOI: 10.1016/j.biortech.2013.09.079

Reference

Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion
Strain(s): 94, 227, 2152, 2173, 2195, 2280
PubMed: 30658785
DOI: 10.1016/j.carbpol.2018.12.057

Otaki, R., Oishi, Y., Abe, S., Fujiwara, S., Sato, N. 2019 Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri. Bioresour. Technol., 289, 121686 (article ID).
Keywords: Carbon metabolism; Chlorella kessleri; Gene expression; Seawater-based biofuel; Triacylglycero
Strain(s): 2152
PubMed: 31238290
DOI: 10.1016/j.biortech.2019.121686

Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2019 The Parachlorella genome and transcriptome endorse active RWP-RK, meiosis and flagellar genes in trebouxiophycean algae. Cytologia, 84, 323-330.
Keywords: Evolution; Flagellum; MID; Meiosis; Parachlorella; RWP-RK
Strain(s): 2152
DOI: 10.1508/cytologia.84.323

Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišová,K., Zachleder, V., Kawano, S. 2018 Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation. Algal Res., 35, 416-426.
Keywords: Parachlorella kessleri; Nutrient dilution; 150-L mass cultivation; Whole genome sequencing; Mutation identification; Single-nucleotide polymorphism
Strain(s): 2152
DOI: 10.1016/j.algal.2018.09.005

Yamazaki, T., Konosu, E., Takeshita, T., Hireta, A., Ota, S., Kazama, Y., Abe, T., Kawano, S. 2018 Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Res., 36, 37-47.
Keywords: Chlorella; Lipids; Starch; Sulfur assimilation; Sulfur starvation
Strain(s): 2152
DOI: 10.1016/j.algal.2018.09.022

Hayashia, T., Otakia, R., Hiraia, K., Tsuzukia, M., Sato, N. 2017 Optimization of seawater-based triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, through simultaneous imposition of loweredtemperature and enhanced-light intensity. Algal Res., 28, 100-107.
Keywords: Chlorella kessleri; Freshwater green algae; Low temperature; Moderate light; Seawater; Triacylglycerol
Strain(s): 2152
DOI: 10.1016/j.algal.2017.10.016

Ota, S., Kawano, S. 2017 Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella. Bio-protocol, 7, e2539 (article ID).
Keywords: Alga; Chlorella; Parachlorella; Phosphorus; Polyphosphate; Molybdenum blue reaction
Strain(s): 2152
DOI: 10.21769/BioProtoc.2539

Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID).
Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM
Strain(s): 2152
PubMed: 26811738
DOI: 10.1186/s13068-016-0424-2

Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID).
Strain(s): 2152
PubMed: 27180903
DOI: 10.1038/srep25731

Takeshita, T., Takeda,K., Ota, S., Yamazaki, T., Kawano, S. 2015 A simple method for measuring the starch and lipid contents in the cell of microalgae. Cytologia, 80, 475-481.
Keywords: Chlorella; Starch; Lipid; Microplate; Trade-off
Strain(s): 2152
DOI: 10.1508/cytologia.80.475

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 2151, 2152, 2159, 2169, 2170, 2179
PubMed: 24583913
DOI: 10.1016/j.biortech.2014.01.135

Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187.
Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri
Strain(s): 2152
PubMed: 24997835

Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Bioresour. Technol., 149, 432-438.
Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation
Strain(s): 2152
PubMed: 24135567
DOI: 10.1016/j.biortech.2013.09.079

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