Strain Data

A big image opens when the thumbnail is clicked.(nies-2152.jpg)

Strain number		NIES-2152
Phylum		Chlorophyta
Class		Trebouxiophyceae
Scientific name		Parachlorella kessleri (Fott & Nováková) Krienitz, E.H.Hegewald, Hepperle, V.Huss, T.Rohr & M.Wolf
Synonym		Chlorella kessleri Fott & Nováková 1969
Former name
Common name		Green alga
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)
History		< IAM (2007) < Yakult < IAM;
Isolator (Date of isolation)		Watanabe, Atsushi
Identified by		Kessler, E. (1994); Confirmed by DNA sequencing at NIES
State of strain		Cryopreservation; Unialgal; Clonal; Axenic[2008 Apr]
Culture condition (Preculture condition)		Medium: C (agar) Temperature: 20 C Light intensity: 8-15 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		Whole-genome ( BBXU00000000 ) , 18S rRNA ( AB488589 )
Cell size (min - max)
Organization		Unicellular
Characteristics		Starch production (Takeshita et al. 2014) ; Genome decoded strain (Ota et al. 2016)
Other strain no.		Other collection strain no. : IAM C-33
Remarks		Cryopreserved; Axenic
Movie

Reference
Kasai, Y., Takagi, S., Ota, S., Ishii, K., Takeshita, T., Kawano, S., Harayama, S. 2024 Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity. Biotechnol. Biofuels Bioprod., 17, (article ID). Keywords: Parachlorella kessleri; Genetic transformationElectroporation; Genome editing; CRISPR/Cas9 Strain(s): 2152 PubMed: 38443960 DOI: 10.1186/s13068-024-02484-7 Ota, S., Yoshimura, K., Kosugi, C., Kawano, S. 2023 Taxonomic and physiological studies of Parachlorella kimitsuensis sp. nov. (Trebouxiophyceae), which shows high ammonium tolerance. Algal Res., 71, 103054 (article ID). Keywords: Chlorella; Halophiles; High ammonium; Parachlorella, Parachlorella kimitsuensis sp. nov.; Remediation; Steelmaking wastewater treatment Strain(s): 2152, 2169, 2273, 2275 DOI: 10.1016/j.algal.2023.103054 Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID). Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod Strain(s): 2151, 2152, 2159, 2169, 2170 PubMed: 34681157 DOI: 10.3390/biology10101060 Anam, K., Rahman, D. Y., Hidhayati, N., Rachmayati, R., Susilaningsih, D., Agustini, N. W. S., Praharyawan, S., Susanti, H., Apriastini, M. 2021 Lipid accumulation on optimized condition through biomass production in green algae. IOP Conf. Ser.: Earth Environ. Sci., 762, 012075 (article ID). Strain(s): 2152, 2169, 2170 DOI: 10.1088/1755-1315/762/1/012075 Delrue, F., Cerqueira, M. R. D., Compadre, A., Alvarez, P., Fleury, G., Escoffier, C., Sassi, J. F. 2021 Hydroponic farm wastewater treatment using an indigenous consortium. Processes, 9, 519 (article ID). Strain(s): 227, 2152 DOI: 10.3390/pr9030519 Inoue, A., Ota, S., Matsuzaki, R., Okada, M., Kawachi, M. 2020 Exploration of high oil-producing microalgae strains using a microfluidic chip cell sorter. Jpn. J. Phycol. (Sôrui), 68, 143-152. Keywords: cell sorting; flow cytometry; on-chip Sort; lipid-producing algae; Nile Red Strain(s): 2152 Otaki, R., Oishi, Y., Abe, S., Fujiwara, S., Sato, N. 2019 Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri. Bioresour. Technol., 289, 121686 (article ID). Keywords: Carbon metabolism; Chlorella kessleri; Gene expression; Seawater-based biofuel; Triacylglycero Strain(s): 2152 PubMed: 31238290 DOI: 10.1016/j.biortech.2019.121686 Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2019 The Parachlorella genome and transcriptome endorse active RWP-RK, meiosis and flagellar genes in trebouxiophycean algae. Cytologia, 84, 323-330. Keywords: Evolution; Flagellum; MID; Meiosis; Parachlorella; RWP-RK Strain(s): 2152 DOI: 10.1508/cytologia.84.323 Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151. Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion Strain(s): 94, 227, 2152, 2173, 2195, 2280 PubMed: 30658785 DOI: 10.1016/j.carbpol.2018.12.057 Yamazaki, T., Konosu, E., Takeshita, T., Hireta, A., Ota, S., Kazama, Y., Abe, T., Kawano, S. 2018 Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Res., 36, 37-47. Keywords: Chlorella; Lipids; Starch; Sulfur assimilation; Sulfur starvation Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.022 Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišová,K., Zachleder, V., Kawano, S. 2018 Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation. Algal Res., 35, 416-426. Keywords: Parachlorella kessleri; Nutrient dilution; 150-L mass cultivation; Whole genome sequencing; Mutation identification; Single-nucleotide polymorphism Strain(s): 2152 DOI: 10.1016/j.algal.2018.09.005 Ota, S. & Kawano, S. 2017 Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella. Bio-protocol, 7, e2539 (article ID). Keywords: Alga; Chlorella; Parachlorella; Phosphorus; Polyphosphate; Molybdenum blue reaction Strain(s): 2152 DOI: 10.21769/BioProtoc.2539 Hayashi, T., Otaki, R., Hirai, K., Tsuzuki, M., Sato, N. 2017 Optimization of seawater-based triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, through simultaneous imposition of loweredtemperature and enhanced-light intensity. Algal Res., 28, 100-107. Keywords: Chlorella kessleri; Freshwater green algae; Low temperature; Moderate light; Seawater; Triacylglycerol Strain(s): 2152 DOI: 10.1016/j.algal.2017.10.016 Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID). Strain(s): 2152 PubMed: 27180903 DOI: 10.1038/srep25731 Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID). Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM Strain(s): 2152 PubMed: 26811738 DOI: 10.1186/s13068-016-0424-2 Takeshita, T., Takeda,K., Ota, S., Yamazaki, T., Kawano, S. 2015 A simple method for measuring the starch and lipid contents in the cell of microalgae. Cytologia, 80, 475-481. Keywords: Chlorella; Starch; Lipid; Microplate; Trade-off Strain(s): 2152 DOI: 10.1508/cytologia.80.475 Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187. Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri Strain(s): 2152 PubMed: 24997835 Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134. Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light Strain(s): 2151, 2152, 2159, 2169, 2170, 2179 PubMed: 24583913 DOI: 10.1016/j.biortech.2014.01.135 Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Bioresour. Technol., 149, 432-438. Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation Strain(s): 2152 PubMed: 24135567 DOI: 10.1016/j.biortech.2013.09.079

Reference

Kasai, Y., Takagi, S., Ota, S., Ishii, K., Takeshita, T., Kawano, S., Harayama, S. 2024 Development of a CRISPR/Cas9-mediated gene-editing method to isolate a mutant of the unicellular green alga Parachlorella kessleri strain NIES-2152 with improved lipid productivity. Biotechnol. Biofuels Bioprod., 17, (article ID).
Keywords: Parachlorella kessleri; Genetic transformationElectroporation; Genome editing; CRISPR/Cas9
Strain(s): 2152
PubMed: 38443960
DOI: 10.1186/s13068-024-02484-7

Ota, S., Yoshimura, K., Kosugi, C., Kawano, S. 2023 Taxonomic and physiological studies of Parachlorella kimitsuensis sp. nov. (Trebouxiophyceae), which shows high ammonium tolerance. Algal Res., 71, 103054 (article ID).
Keywords: Chlorella; Halophiles; High ammonium; Parachlorella, Parachlorella kimitsuensis sp. nov.; Remediation; Steelmaking wastewater treatment
Strain(s): 2152, 2169, 2273, 2275
DOI: 10.1016/j.algal.2023.103054

Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID).
Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod
Strain(s): 2151, 2152, 2159, 2169, 2170
PubMed: 34681157
DOI: 10.3390/biology10101060

Anam, K., Rahman, D. Y., Hidhayati, N., Rachmayati, R., Susilaningsih, D., Agustini, N. W. S., Praharyawan, S., Susanti, H., Apriastini, M. 2021 Lipid accumulation on optimized condition through biomass production in green algae. IOP Conf. Ser.: Earth Environ. Sci., 762, 012075 (article ID).
Strain(s): 2152, 2169, 2170
DOI: 10.1088/1755-1315/762/1/012075

Delrue, F., Cerqueira, M. R. D., Compadre, A., Alvarez, P., Fleury, G., Escoffier, C., Sassi, J. F. 2021 Hydroponic farm wastewater treatment using an indigenous consortium. Processes, 9, 519 (article ID).
Strain(s): 227, 2152
DOI: 10.3390/pr9030519

Inoue, A., Ota, S., Matsuzaki, R., Okada, M., Kawachi, M. 2020 Exploration of high oil-producing microalgae strains using a microfluidic chip cell sorter. Jpn. J. Phycol. (Sôrui), 68, 143-152.
Keywords: cell sorting; flow cytometry; on-chip Sort; lipid-producing algae; Nile Red
Strain(s): 2152

Otaki, R., Oishi, Y., Abe, S., Fujiwara, S., Sato, N. 2019 Regulatory carbon metabolism underlying seawater-based promotion of triacylglycerol accumulation in Chlorella kessleri. Bioresour. Technol., 289, 121686 (article ID).
Keywords: Carbon metabolism; Chlorella kessleri; Gene expression; Seawater-based biofuel; Triacylglycero
Strain(s): 2152
PubMed: 31238290
DOI: 10.1016/j.biortech.2019.121686

Ota, S., Oshima, K., Yamazaki, T., Takeshita, T., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2019 The Parachlorella genome and transcriptome endorse active RWP-RK, meiosis and flagellar genes in trebouxiophycean algae. Cytologia, 84, 323-330.
Keywords: Evolution; Flagellum; MID; Meiosis; Parachlorella; RWP-RK
Strain(s): 2152
DOI: 10.1508/cytologia.84.323

Mathiot, C., Ponge, P., Gallard, B., Sassi, J.-F., Delrue, F., Le Moigne, N. 2019 Microalgae starch-based bioplastics: screening of ten strains and plasticization of unfractionated microalgae by extrusion. Carbohydr. Polym., 208/, 142-151.
Keywords: Microalgae; Bioplastics; Starch; Plasticization; Extrusion
Strain(s): 94, 227, 2152, 2173, 2195, 2280
PubMed: 30658785
DOI: 10.1016/j.carbpol.2018.12.057

Yamazaki, T., Konosu, E., Takeshita, T., Hireta, A., Ota, S., Kazama, Y., Abe, T., Kawano, S. 2018 Independent regulation of the lipid and starch synthesis pathways by sulfate metabolites in the green microalga Parachlorella kessleri under sulfur starvation conditions. Algal Res., 36, 37-47.
Keywords: Chlorella; Lipids; Starch; Sulfur assimilation; Sulfur starvation
Strain(s): 2152
DOI: 10.1016/j.algal.2018.09.022

Takeshita, T., Ivanov, I. N., Oshima, K., Ishii, K., Kawamoto, H., Ota, S., Yamazaki, T., Hirata, A., Kazama, Y., Abe, T., Hattori, M., Bišová,K., Zachleder, V., Kawano, S. 2018 Comparison of lipid productivity of Parachlorella kessleri heavy-ion beam irradiation mutant PK4 in laboratory and 150-L mass bioreactor, identification and characterization of its genetic variation. Algal Res., 35, 416-426.
Keywords: Parachlorella kessleri; Nutrient dilution; 150-L mass cultivation; Whole genome sequencing; Mutation identification; Single-nucleotide polymorphism
Strain(s): 2152
DOI: 10.1016/j.algal.2018.09.005

Ota, S. & Kawano, S. 2017 Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella. Bio-protocol, 7, e2539 (article ID).
Keywords: Alga; Chlorella; Parachlorella; Phosphorus; Polyphosphate; Molybdenum blue reaction
Strain(s): 2152
DOI: 10.21769/BioProtoc.2539

Hayashi, T., Otaki, R., Hirai, K., Tsuzuki, M., Sato, N. 2017 Optimization of seawater-based triacylglycerol accumulation in a freshwater green alga, Chlorella kessleri, through simultaneous imposition of loweredtemperature and enhanced-light intensity. Algal Res., 28, 100-107.
Keywords: Chlorella kessleri; Freshwater green algae; Low temperature; Moderate light; Seawater; Triacylglycerol
Strain(s): 2152
DOI: 10.1016/j.algal.2017.10.016

Ota, S., Yoshihara, M., Yamazaki, T., Takeshita, T., Hirata, A., Konomi, M., Oshima, K., Hattori, M., Bišová, K., Zachleder, V., Kawano, S. 2016 Deciphering the relationship among phosphate dynamics, electron-dense body and lipid accumulation in the green alga Parachlorella kessleri. Sci Rep, 6, 25731 (article ID).
Strain(s): 2152
PubMed: 27180903
DOI: 10.1038/srep25731

Ota, S., Oshima, K., Yamazaki, T., Kim, S., Yu, Z., Yoshihara, M., Takeda, K., Takeshita, T., Hirata, A., Bišová, K., Zachleder, V., Hattori, M., Kawano, S. 2016 Highly efficient lipid production in the green alga Parachlorella kessleri: draft genome and transcriptome endorsed by whole-cell 3D ultrastructure. Biotechnol. Biofuels, 9, 13 (article ID).
Keywords: Genome; Green alga; Lipid body; Parachlorella kessleri; RNA-seq; Whole-genome sequence; Transcriptome; 3D-TEM
Strain(s): 2152
PubMed: 26811738
DOI: 10.1186/s13068-016-0424-2

Takeshita, T., Takeda,K., Ota, S., Yamazaki, T., Kawano, S. 2015 A simple method for measuring the starch and lipid contents in the cell of microalgae. Cytologia, 80, 475-481.
Keywords: Chlorella; Starch; Lipid; Microplate; Trade-off
Strain(s): 2152
DOI: 10.1508/cytologia.80.475

Yamazaki, T; Hirai, C; Ota, S; Kuwano, K; Kawano, S. 2014 Use of FM1-43, a membrane-specific fluorescent dye, to estimate plasma membrane integrity in the cryopreservation of green algae. CryoLetters, 35, 180-187.
Keywords: FM1-43, cryopreservation, two-step freezing, plasma membrane, green algae, Parachlorella kessleri
Strain(s): 2152
PubMed: 24997835

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 2151, 2152, 2159, 2169, 2170, 2179
PubMed: 24583913
DOI: 10.1016/j.biortech.2014.01.135

Ota, S., Matsuda, T., Takeshita, T., Yamazaki, T., Kazama, Y., Abe, T., Kawano, S. 2013 Phenotypic spectrum of Parachlorella kessleri (Chlorophyta) mutants produced by heavy-ion irradiation. Bioresour. Technol., 149, 432-438.
Keywords: Algae; Breeding; Parachlorella; Heavy-ion irradiation; Mutation
Strain(s): 2152
PubMed: 24135567
DOI: 10.1016/j.biortech.2013.09.079

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