Strain number NIES-2151  
Phylum Chlorophyta  
Class Chlorophyceae  
Scientific name Graesiella emersonii (Shihira & Krauss) Nozaki et al.  
Synonym Chlorella emersonii Shihira & Krauss; Chlorella fusca Shihira & Krauss var. vacuolata Shihira & Krauss; Graesiella vacuolata (Shihira & Krauss) Kalina & Puncochárová  
Former name Chlorella pyrenoidosa Chick  
Common name Green alga  
Locality (Date of collection) Berlin-Dahlem, Germany  
Latitude / Longitude 52.447941 / 13.284166 
Habitat (Isolation source) Freshwater (Tap water)  
History < IAM (2007) < BIU (UTEX; 1961);  
Isolator (Date of isolation) Emerson, R. (1926-**-**)  
Identified by Kessler, E.; Confirmed by DNA sequencing at NIES  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 C
Light intensity:  8-15 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M  
Gene information 18S rRNA ( AB488562 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Starch production (Takeshita et al. 2014)  
Other strain no. Other collection strain no. : IAM C-105; CCAP 211/8C; SAG 211-8c; UTEX 252; UTCC 89; NIBB 4001; NIBB 4002; NIBB 4003; NIBB 4004; NIBB 4005. NIBB 4006
Remarks Cryopreserved; Axenic 
Noguchi, M., Aizawa, R., Nakazawa, D., Hakumura, Y., Furuhashi, Y., Yang, S., Ninomiya, K.,Takahashi, K., Honda, R. 2021 Application of real treated wastewater to starch production by microalgae: Potential effect of nutrients and microbial contamination. Biochem. Eng. J., 169, 107973 (article ID).
Keywords: Biorefinery; Chlorella vulgaris; Chlorination; Microbial contamination; Municipal wastewater; Starch (carbohydrate) production
Strain(s): 215121592170 
DOI: 10.1016/j.bej.2021.107973

Maltsev, Y., Maltseva, K., Kulikovskiy, M., Maltseva, S. 2021 Influence of light conditions on microalgae growth and content of lipids, carotenoids, and fatty acid composition. Biology-Basel, 10, 1060 (article ID).
Keywords: carotenoids; fatty acids; light; lipids; microalgae; photoperiod
Strain(s): 21512152215921692170 
PubMed: 34681157
DOI: 10.3390/biology10101060

Kawasaki, S., Yoshida, R., Ohkoshi, K., Toyoshima, H., 2020 Coelastrella astaxanthina sp. nov. (Sphaeropleales, Chlorophyceae), a novel microalga isolated from an asphalt surface in midsummer in Japan. Phycol. Res., 68, 107-114.
Keywords: astaxanthin; dry conditions; photo-oxidative stress; Scenedesmaceae; terrestrial microalgae
Strain(s): 2151 
DOI: 10.1111/pre.12412

Htet, AN., Noguchi, M., Ninomiya, K., Tsuge, Y., Kuroda, K., Kajita, S., Masai, E., Katayama, Y., Shikinaka, K., Otsuka, Y., Nakamura, M., Honda, R., Takahashi, K. 2018 Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid. J. Biosci. Bioeng., 125, 717-722.
Keywords: Microalgae; Chlorella emersonii; Acid hydrolysis; Fermentation; Escherichia coli; 2-pyrone 4,6-dicarboxylic acid
Strain(s): 2151 
PubMed: 29395960
DOI: 10.1016/j.jbiosc.2017.12.026

Kishi, M., Kawai, M., Toda, T. 2015 Heterotrophic utilization of ethylene glycol and propylene glycol by Chlorella protothecoides. Algal Res., 11, 428-434.
Keywords: algae; Chlorella protothecoides; Ethylene glycol; Propylene glycol; Heterotrophic; pH
Strain(s): 215121682170 
DOI: 10.1016/j.algal.2015.04.020

Takeshita, T., Ota, S., Yamazaki, T., Hirata, A., Zachleder, V., Kawano, S. 2014 Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions. Bioresour. Technol., 158, 127-134.
Keywords: Accumulation shift; Lipid; Chlorella; Starch; Strong light
Strain(s): 215121522159216921702179 
PubMed: 24583913
DOI: 10.1016/j.biortech.2014.01.135

Kessler, E. & Huss, V. A. R. 1992 Comparative physiology and biochemistry and taxonomic assignment of the Chlorella (Chlorophyceae) strains of the Culture Collection of the University of Texas at Austin. J. Phycol., 28, 550-553.
Keywords: chemotaxonomy; Chlorella; Chlorophyta; UTEX culture collection; utilization for research and biotechnology
Strain(s): 6866872150215121602163216921752176 
DOI: 10.1111/j.0022-3646.1992.00550.x

Brand, J. J. & Wright, J. N. 1989 Hydrogen production by eukaryotic algae. Biotech. Bioeng., 33, 1482-1488.
Strain(s): 2151216921902194220122022203220422112213221922792291 

Maruyama, K. 1977 Classification of Chlorella strain by infrared absorption spectra of cell samples. Bot. Mag. Tokyo, 90, 67-77.
Strain(s): 2262276856872151216321702175 
DOI: 10.1007/BF02489470

Maruyama, K. 1977 Classification of Chlorella strains by cell appearance and group sera. Bot. Mag. Tokyo, 90, 57-66.
Strain(s): 2262276852151216321702175 
DOI: 10.1007/BF02489469

Sargent, D. F. & Taylor, C. P. S. 1972 Light-induced inhibition of respiration in DCMU-poisoned Chlorella caused by phytosystem I activity. Can. J. Bot., 50, 13-21.
Strain(s): 2151 
DOI: 10.1139/b72-003

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.