Strain number NIES-2146  
Phylum Heterokontophyta  
Class Eustigmatophyceae  
Scientific name Nannochloropsis oceanica Suda & Miyashita  
Former name Formerly identified as Nannochloropsis oculata (Droop) Hibberd, re-identified by DNA analysis[2022 Oct]  
Common name  
Locality (Date of collection) Institute of Fisheries, Nagasaki, Nagasaki, Japan  
Latitude / Longitude  
Habitat (Isolation source) Marine  
History < IAM (2007) < Maruyama, Ko (around 1986)  
Isolator (Date of isolation) Maruyama, Ko (1986-**-**)  
Identified by Maruyama, Ko, et al.  
State of strain Cryopreservation; Unialgal; Clonal; Axenic[2018 Feb]  
Culture condition
(Preculture condition)
Medium:  ESM (agar)  
Temperature:  20 C
Light intensity:  5-9 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  4 M  
Gene information 18S rRNA ( LC730857 )  
Cell size (min - max) 3 - 8 μm  
Organization Unicellular 
Characteristics Live food (Wullur et al. 2011)  
Other strain no. Other collection strain no. : IAM ST-6
Remarks Cryopreserved; Axenic 
Pilátová, J., Pánek, T., Oborník, M., Čepička, I., Mojzeš, P. 2022 Revisiting biocrystallization: purine crystalline inclusions are widespread in eukaryotes. ISME J., 16, 2290-2294.
Strain(s): 673039951604214622852860 
PubMed: 35672454
DOI: 10.1038/s41396-022-01264-1

Strain(s): 244714421462160 

Sun, H., Wu, T., Chen, H Y., Ren, Y., Yang, S., Huang, J., Mou, H., Chen, F. 2021 Powerful tools for productivity improvements in microalgal production. Renew. Sust. Energ. Rev., 152, 111609 (article ID).
Keywords: Microalgal production; Productivity improvement; Kinetic model; Metabolic flux analysis; Genetic tool; Lipid biosynthesis
Strain(s): 14421402146 
DOI: 10.1016/j.rser.2021.111609

Shikata, T., Taniguchi, E., Sakamoto, S., Kitatsuji, S., Yamasaki, Y., Yoshida, M., Oikawa, H. 2020 Phylogeny, growth and toxicity of the noxious red-tide dinoflagellate Alexandrium leei in Japan. Reg. Stud. Mar. Sci., 36, 101265 (article ID).
Keywords: Fish; Haemolytic activity; Photoinhibition; rDNA; Rotifer; Temperature
Strain(s): 2146 
DOI: 10.1016/j.rsma.2020.101265

Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399.
Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes
Strain(s): 21452146 
PubMed: 29511798
DOI: 10.1007/s00299-018-2270-0

Shih, C., Chen, H., Lee, H., Tsai, H. 2015 Purple chromoprotein gene serves as a new selection marker for transgenesis of the microalga Nannochloropsis oculata. PLoS One, 10, e0120780 (article ID).
Strain(s): 2146 
PubMed: 25793255
DOI: 10.1371/journal.pone.0120780

Fukuda, S., Iwamoto, K., Atsumi, M., Yokoyama, A., Nakayama, T., Ishida, K-I., Inouye, I., Shiraiwa, Y. 2014 Global searches for microalgae and aquatic plants that can eliminate radioactive cesium, iodine and strontium from the radio-polluted aquatic environment: a bioremediation strategy. J. Plant Res., 127, 79-89.
Keywords: Algal phytoremediation; Bioaccumulation; Radiopollution; Radionuclide elimination; Radioactive cesium; The Fukushima 1 Nuclear Power Plant accident
Strain(s): 243671155160203233246329333391405417440487503529531538548553587597678727805843859931995100310121015101710311044104512591326133113771382138814111435143914401441144214581728182618311833184018461862186519562131214421462147215021752176218522682325234123522377241124122437285428552856285828592860 
PubMed: 24346654
DOI: 10.1007/s10265-013-0596-9

Wullur, S., Sakakura, Y., Hagiwara, A. 2011 Application of the minute monogonont rotifer Proales similis de Beauchamp in larval rearing of seven-band grouper epinephelus septemfasciatus. Aquaculture, 315, 355-360.
Keywords: Rotifer; Proales similis; Brachionus rotundiformis; Live food; Fatty acid; Epinephelus septemfasciatus
Strain(s): 2146 
DOI: 10.1016/j.aquaculture.2011.02.025

Maruyama, I., Nakamura, T., Matubayashi, T., Ando, Y., Maeda, T. 1986 Identification of the alga known as "marine Chlorella" as a member of the Eustigmatophyceae. Jpn. J. Phycol. (Sôrui), 34, 319-325.
Strain(s): 2146 

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