Strain Data

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Strain number		NIES-2145
Phylum		Heterokontophyta
Class		Eustigmatophyceae
Scientific name		Nannochloropsis oceanica Suda & Miyashita
Synonym
Former name		Formerly identified as Nannochloropsis oculata (Droop) Hibberd, re-identified by DNA analysis[2017]
Common name
Locality (Date of collection)
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< IAM (2007) < Hara, Yoshiaki (1988) < Suisan Center of Fukushima Prefecture
Isolator (Date of isolation)
Identified by
State of strain		Subculture; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: ESM (agar) Temperature: 20 C Light intensity: 5-9 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 4 M
Gene information
Cell size (min - max)
Organization		Unicellular
Characteristics		oil (hydrocarbon) production ; TAG (Nobusawa et al.)
Other strain no.		Other collection strain no. : IAM ST-4
Remarks
Movie

Reference
Kurita, T., Moroi, K., Iwai, M., Okazaki, K., Shimizu, S., Nomura, S., Saito, F., Maeda, S., Takami, A., Sakamoto, A., Ohta, H., Sakuma, T., Yamamoto, T. 2020 Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES‐2145. Genes Cells, 25, 695-702. Strain(s): 2145 PubMed: 32888368 DOI: 10.1111/gtc.12805 Saito, T., Ichihara, T., Inoue, H., Uematsu, T., Hamada, S., Watanabe, T., Takimura, Y., Webb, J. 2020 Comparison of areal productivity of Nannochloropsis oceanica between lab-scale and industrial-scale raceway pond. Mar. Biotechnol., 22, 836–841. Keywords: Microalgae production; Nannochloropsis; Open raceway pond; Sequential batch culture Strain(s): 2145 PubMed: 32860094 DOI: 10.1007/s10126-020-09990-3 Shimakawa, G., Murakami, A., Niwa, K., Matsuda, Y., Wada, A., Miyake, C. 2019 Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs. Photosynth. Res., 139, 401–411. Keywords: Reactive oxygen species; P700 oxidation; Photosystem I; Seaweeds Strain(s): 705, 1959, 2138, 2145, 2147 PubMed: 29845382 DOI: 10.1007/s11120-018-0522-z Sugihara, S., Ozaki, T., Tojo, T., Endo, H., Saito, T., Takimura, Y. 2019 Identification of novel 3-ketoacyl-acyl carrier protein synthase involved in producing medium chain fatty acids from microalgae. Bioresour. Technol. Reports, 7, 100184 (article ID). Keywords: 3-ketoacyl ACP synthase; Medium chain fatty acid; Microalgae; Nannochloropsis Strain(s): 2145 DOI: 10.1016/j.biteb.2019.03.016 Nobusawa, T., Yamakawa-Ayukawa, K., Saito, F., Nomura, S., Takami, A., Ohta, H. 2019 A homolog of Arabidopsis SDP1 lipase in Nannochloropsis is involved in degradation of de novo-synthesized triacylglycerols in the endoplasmic reticulum. Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1864, 1185-1193. Keywords: Nannochloropsis; Lipase; Triacylglycerol; SDP1 Strain(s): 2145 PubMed: 31152796 DOI: 10.1016/j.bbalip.2019.05.013 Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary). Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution Strain(s): 39, 2145, 2168, 2170, 2256, 2257, 2258 DOI: 10.2965/jswe.42.239 Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399. Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes Strain(s): 2145, 2146 PubMed: 29511798 DOI: 10.1007/s00299-018-2270-0 Nobusawa, T., Hori, K., Mori, H., Kurokawa, K., Ohta, H. 2017 Differently localized lysophosphatidic acid acyltransferasescrucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis. Plant J., 90, 547–559. Keywords: Nannochloropsis; triacylglycerol (TAG); biofuels; lysophosphatidic acid acyltransferase (LPAT); lipid droplets Strain(s): 2145 PubMed: 28218992 DOI: 10.1111/tpj.13512 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 3391 Iwai, M., Hori, K., Sasaki-Sekimoto, Y., Shimojima, M., Ohta, H. 2015 Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii. Front. Microbiol. 6, 912 (article ID). Keywords: algae; Nannochloropsis; phosphorus starvation; inducible promoter; triacylglycerol Strain(s): 2145 PubMed: 26441858 DOI: 10.3389/fmicb.2015.00912 Nakanishi, K., Deuchi, K., Kuwano, K. 2012 Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage. J. Appl. Phycol., 24, 1381-1385. Keywords: Cryopreservation; Cryoprotectant; Chlorella vulgaris; Nannochloropsis oculata; Tetraselmis tetrathele; Chlorophyll Strain(s): 2145, 2170 DOI: 10.1007/s10811-012-9790-8 Sharifah, E. N., Eguchi, M. 2011 The phytoplankton Nannochloropsis oculata enhances the ability ofRoseobacter clade bacteria to inhibit the growth of Vibrio anguillarum. PLoS One, 6, e26756 (article ID). Strain(s): 2145 PubMed: 22053210 DOI: 10.1371/journal.pone.0026756

Reference

Kurita, T., Moroi, K., Iwai, M., Okazaki, K., Shimizu, S., Nomura, S., Saito, F., Maeda, S., Takami, A., Sakamoto, A., Ohta, H., Sakuma, T., Yamamoto, T. 2020 Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga, Nannochloropsis oceanica NIES‐2145. Genes Cells, 25, 695-702.
Strain(s): 2145
PubMed: 32888368
DOI: 10.1111/gtc.12805

Saito, T., Ichihara, T., Inoue, H., Uematsu, T., Hamada, S., Watanabe, T., Takimura, Y., Webb, J. 2020 Comparison of areal productivity of Nannochloropsis oceanica between lab-scale and industrial-scale raceway pond. Mar. Biotechnol., 22, 836–841.
Keywords: Microalgae production; Nannochloropsis; Open raceway pond; Sequential batch culture
Strain(s): 2145
PubMed: 32860094
DOI: 10.1007/s10126-020-09990-3

Shimakawa, G., Murakami, A., Niwa, K., Matsuda, Y., Wada, A., Miyake, C. 2019 Comparative analysis of strategies to prepare electron sinks in aquatic photoautotrophs. Photosynth. Res., 139, 401–411.
Keywords: Reactive oxygen species; P700 oxidation; Photosystem I; Seaweeds
Strain(s): 705, 1959, 2138, 2145, 2147
PubMed: 29845382
DOI: 10.1007/s11120-018-0522-z

Sugihara, S., Ozaki, T., Tojo, T., Endo, H., Saito, T., Takimura, Y. 2019 Identification of novel 3-ketoacyl-acyl carrier protein synthase involved in producing medium chain fatty acids from microalgae. Bioresour. Technol. Reports, 7, 100184 (article ID).
Keywords: 3-ketoacyl ACP synthase; Medium chain fatty acid; Microalgae; Nannochloropsis
Strain(s): 2145
DOI: 10.1016/j.biteb.2019.03.016

Nobusawa, T., Yamakawa-Ayukawa, K., Saito, F., Nomura, S., Takami, A., Ohta, H. 2019 A homolog of Arabidopsis SDP1 lipase in Nannochloropsis is involved in degradation of de novo-synthesized triacylglycerols in the endoplasmic reticulum. Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1864, 1185-1193.
Keywords: Nannochloropsis; Lipase; Triacylglycerol; SDP1
Strain(s): 2145
PubMed: 31152796
DOI: 10.1016/j.bbalip.2019.05.013

Komatsu, K., Onouchi, H., Imai, A., Kawasaki, N., Hashim, E. F., Mohd Rajuddin, M. K. 2019 Effects of dissolved organic matter in soil extracts on the growth of microalgae. J. Jpn. Soc. Wat. Environ., 42, 239-246 (in Japanese with English summary).
Keywords: Soil extracts; Microalgae; Microplate technique; Excitation emission matrix; Molecular size distribution
Strain(s): 39, 2145, 2168, 2170, 2256, 2257, 2258
DOI: 10.2965/jswe.42.239

Pollner, E., Farré, E. M., Bennlng, C. 2018 Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp. Plant Cell Reports, 37, 1383-1399.
Keywords: Nannochloropsis; Algal biotechnology; Marker-free engineering; Gene stacking; Synthetic biology; Episomes
Strain(s): 2145, 2146
PubMed: 29511798
DOI: 10.1007/s00299-018-2270-0

Nobusawa, T., Hori, K., Mori, H., Kurokawa, K., Ohta, H. 2017 Differently localized lysophosphatidic acid acyltransferasescrucial for triacylglycerol biosynthesis in the oleaginous alga Nannochloropsis. Plant J., 90, 547–559.
Keywords: Nannochloropsis; triacylglycerol (TAG); biofuels; lysophosphatidic acid acyltransferase (LPAT); lipid droplets
Strain(s): 2145
PubMed: 28218992
DOI: 10.1111/tpj.13512

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 3391

Iwai, M., Hori, K., Sasaki-Sekimoto, Y., Shimojima, M., Ohta, H. 2015 Manipulation of oil synthesis in Nannochloropsis strain NIES-2145 with a phosphorus starvation–inducible promoter from Chlamydomonas reinhardtii. Front. Microbiol. 6, 912 (article ID).
Keywords: algae; Nannochloropsis; phosphorus starvation; inducible promoter; triacylglycerol
Strain(s): 2145
PubMed: 26441858
DOI: 10.3389/fmicb.2015.00912

Nakanishi, K., Deuchi, K., Kuwano, K. 2012 Cryopreservation of four valuable strains of microalgae, including viability and characteristics during 15 years of cryostorage. J. Appl. Phycol., 24, 1381-1385.
Keywords: Cryopreservation; Cryoprotectant; Chlorella vulgaris; Nannochloropsis oculata; Tetraselmis tetrathele; Chlorophyll
Strain(s): 2145, 2170
DOI: 10.1007/s10811-012-9790-8

Sharifah, E. N., Eguchi, M. 2011 The phytoplankton Nannochloropsis oculata enhances the ability ofRoseobacter clade bacteria to inhibit the growth of Vibrio anguillarum. PLoS One, 6, e26756 (article ID).
Strain(s): 2145
PubMed: 22053210
DOI: 10.1371/journal.pone.0026756

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