A big image opens when the thumbnail is clicked.(nies-2142.jpg)
Strain number NIES-2142  
Phylum Heterokontophyta  
Class Chrysophyceae  
Scientific name Ochromonas danica Pringsheim  
Synonym  
Former name  
Common name  
Locality (Date of collection) near Everdrup, Denmark  
Latitude / Longitude 55.203341 / 11.94647 
Habitat (Isolation source) Freshwater  
History < IAM (2007) < UTEX (1996);  
Isolator (Date of isolation) Pringsheim, E. G. (1954-**-**)  
Identified by Pringsheim, E. G.  
State of strain Subculture; Unialgal; Clonal; Axenic[2018 Mar]  
Culture condition
(Preculture condition)
Medium:  O (semi-solid)  
Temperature:  20 C
Light intensity:  13-16 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information ITS1-5.8S-ITS2 ( Y07976 ) , 16S rRNA ( X53183 ) , 28S rRNA ( KM590767 GU935636 Y07977 ) , gapC ( AJ512345 ) , gapC1 ( AJ512344 ) , psaA ( KM590836 ) , rbcL ( GU935657 ) , vppa ( AJ251725 ) , odbgp ( LC140879 )  
Cell size (min - max)  
Organization  
Characteristics Mixotrophic ; Phagotrophic  
Other strain no. Other collection strain no. : IAM CS-4 (=CS-2); ATCC 30004; CCAP 933/2; SAG 933-7; UTEX L 1298; CCAP 933/2B; CCMP 585
 
Remarks Axenic 
Movie https://www.youtube.com/watch?v=bEN7IN47VNA&list=PLFTJlLsZcVbiJY3ygYAPKeVOfDdOh9UEW&index=23  
Reference
Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 189141517711152232252332342652742752762772782792802812822842933233243303333443453463473483503533723773883913954074084094134144174614624664875345485535565575585595605625875885895906036056226236956966976986997007017027037047057067077087097107117127137147157167417657667678058379971001100210031004100510061007100910111016101710441045104610471302130313241327133013391340134913531370137513761379138313841385138613871391139213931395139813991400140116991700173018131815181618261827183118621863186418651874196319641965197419751976214221432144214521472148230023312332235123632364236523692370237625062533253425352536253725902633266826892690269126932694269626972707271627172718272027222723272527262729273027312732277027712772277328392840284128422843284428592872287828903391 

Yamamoto, Y., Kawashima, D., Hashizume, A., Hisamotsu, M., Isono, N. 2013 Purification and characterization of 1,3-β-D-glucan phosphorylase from Ochromonas danica. Biosci. Biotechnol. Biochem., 77, 130411-130416.
Keywords: 1,3-β-D-glucan phosphorylase; Ochromonas danica; laminarin; laminarioligosaccharides; 1,3-β-D-glucan
Strain(s): 2142 
PubMed: 24018693
DOI: 10.1271/bbb.130411

Kawahara, T., Kumaki, Y., Kamada, T., Ishii, T., Okino, T. 2009 Absolute configuration of chlorosulfolipids from the chrysophyta Ochromonas danica. J. Org. Chem., 74, 6016-6024.
Strain(s): 2142 
PubMed: 19627129
DOI: 10.1021/jo900860e

McKay, R. M., Gibbs, S. P. 1989 Immunocytochemical localization of ribulose 1,5-bisphosphate carboxylase/oxygenase in light-limited and light-saturated cells of Chlorella pyrenoidosa. Protoplasma, 149, 31-37.
Keywords: pyrenoid; ribulose 1,5-bisphosphate carboxylase/oxygenase; Chlorella pyrenoidosa; immunocytochemistry
Strain(s): 6872142 

Witt, D., Stackebrandt, E. 1988 Disproving the hypothesis of a common ancestry for the Ochromonas danica chrysoplast and Heliobacterium chlorum. Arch. Mikrobiol., 150, 244-248.
Strain(s): 2142 
DOI: 10.1007/BF00407787

Coleman, A. W. 1988 The autofluorescent flagellum: a new phylogenetic enigma. J. Phycol., 24, 118-120.
Keywords: autofluorescence; Chrysophyceae; flagellum; fluorescence; heterokont; Ochromonas; photoreceptor; Prymnesium
Strain(s): 21422144 
DOI: 10.1111/j.1529-8817.1988.tb04465.x

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