Strain Data

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Strain number		NIES-2087
Phylum		Cyanophyta
Class		Cyanophyceae
Scientific name		Prochlorococcus marinus S.W.Chisholm, S.L.Frankel, R.Goericke, R.J.Olson, B.Palenik, J.B.Waterbury, L.West-Johnsrud & E.R.Zettler
Synonym
Former name
Common name		Blue-green alga ; Cyanobacteria ; Picoplankton
Locality (Date of collection)		East Mediterranean Sea
Latitude / Longitude		35 / 20
Habitat (Isolation source)		Marine (Seawater)
History		< Kawachi, Masanobu < CCMP
Isolator (Date of isolation)		Vaulot, D. (1991-03-01)
Identified by		Vaulot, D.
State of strain		Cryopreservation; Unialgal; Clonal; Non-axenic
Culture condition (Preculture condition)		Medium: PRO-99 Temperature: 20 C Light intensity: 7-11 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M
Gene information		Whole-genome ( BX548174 )
Cell size (min - max)		1 - 3 μm
Organization		Unicellular; Non-flagellate
Characteristics		Genome decoded strain
Other strain no.		Other collection strain no. : CCMP 1986; CCMP 1378 Other strain no. : MED4
Remarks		Cryopreserved
Movie

Reference
Sekiguchi, Y., Kobayashi, A., Takayama, Y., Oide, M., Fukuda, A., Yamamoto, Y., Okajima, K., Oroguchi, T., Hirakawa, T., Inui, Y., Matsunaga, S., Yamamoto, M., Nakasako, M. 2018 Coherent X-ray Diffraction Imaging of Cyanidioschyzon merolae. In Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology, Eds. by Kuroiwa, T., Miyagishima, S., Matsunaga, S., Sato, N., Nozaki, H., Tanaka, K., Misumi, O., Springer Singapore, pp. 153-173. Strain(s): 2087 DOI: 10.1007/978-981-10-6101-1_10 Kobayashi, A., Sekiguchi, Y., Oroguchi, T., Okajima, K., Fukuda, A., Oide,, M., Yamamoto, M., Nakasako, M. 2016 Specimen preparation for cryogenic coherent X-ray diffraction imaging of biological cells and cellular organelles by using the X-ray free-electron laser at SACLA. J. Synchrot. Radiat., 23, 975-989. Keywords: coherent X-ray diffraction imaging; X-ray free-electron laser; frozen-hydrated non-crystalline specimens; cryogenic X-ray diffraction experiment; structures of cells and cellular organelles Strain(s): 2087 PubMed: 27359147 DOI: 10.1107/S1600577516007736 Komatsu, H., Wada, K., Kanjoh, T., Miyashita, H., Sato, M., Kawachi, M., Kobayashi, M. 2016 Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086.” Photosynth. Res., 130, 445-467. Keywords: Chlorophyll; Divinyl chlorophyll; Pheophytin; Photosynthesis; Prochlorococcus Strain(s): 2086, 2087, 2884, 3376 PubMed: 27334004 DOI: 10.1007/s11120-016-0283-5 Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2016 Additional analysis of cyanobacterial polyamines ─ Distributions of spermidine, homospermidine, spermine, and thermospermine within the phylum Cyanobacteria. ─ Microb. Resour. Syst., 32, 179-186. Keywords: cyanobacteria; homospermidine; polyamine; thermospermine Strain(s): 2086, 2087, 2134, 2412, 2539, 3373

Reference

Sekiguchi, Y., Kobayashi, A., Takayama, Y., Oide, M., Fukuda, A., Yamamoto, Y., Okajima, K., Oroguchi, T., Hirakawa, T., Inui, Y., Matsunaga, S., Yamamoto, M., Nakasako, M. 2018 Coherent X-ray Diffraction Imaging of Cyanidioschyzon merolae. In Cyanidioschyzon merolae: A New Model Eukaryote for Cell and Organelle Biology, Eds. by Kuroiwa, T., Miyagishima, S., Matsunaga, S., Sato, N., Nozaki, H., Tanaka, K., Misumi, O., Springer Singapore, pp. 153-173.
Strain(s): 2087
DOI: 10.1007/978-981-10-6101-1_10

Kobayashi, A., Sekiguchi, Y., Oroguchi, T., Okajima, K., Fukuda, A., Oide,, M., Yamamoto, M., Nakasako, M. 2016 Specimen preparation for cryogenic coherent X-ray diffraction imaging of biological cells and cellular organelles by using the X-ray free-electron laser at SACLA. J. Synchrot. Radiat., 23, 975-989.
Keywords: coherent X-ray diffraction imaging; X-ray free-electron laser; frozen-hydrated non-crystalline specimens; cryogenic X-ray diffraction experiment; structures of cells and cellular organelles
Strain(s): 2087
PubMed: 27359147
DOI: 10.1107/S1600577516007736

Komatsu, H., Wada, K., Kanjoh, T., Miyashita, H., Sato, M., Kawachi, M., Kobayashi, M. 2016 Unique chlorophylls in picoplankton Prochlorococcus sp. “Physicochemical properties of divinyl chlorophylls, and the discovery of monovinyl chlorophyll b as well as divinyl chlorophyll b in the species Prochlorococcus NIES-2086.” Photosynth. Res., 130, 445-467.
Keywords: Chlorophyll; Divinyl chlorophyll; Pheophytin; Photosynthesis; Prochlorococcus
Strain(s): 2086, 2087, 2884, 3376
PubMed: 27334004
DOI: 10.1007/s11120-016-0283-5

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2016 Additional analysis of cyanobacterial polyamines ─ Distributions of spermidine, homospermidine, spermine, and thermospermine within the phylum Cyanobacteria. ─ Microb. Resour. Syst., 32, 179-186.
Keywords: cyanobacteria; homospermidine; polyamine; thermospermine
Strain(s): 2086, 2087, 2134, 2412, 2539, 3373

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