A big image opens when the thumbnail is clicked.(nies-0068.jpg)
Strain number NIES-68  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium peracerosum-strigosum-littorale complex  
Former name  
Common name Desmid  
Locality (Date of collection) Damchan, Nepal (1965-11-05)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Pool soil)  
History < Ichimura, Terunobu  
Isolator (Date of isolation) Ichimura, Terunobu  
Identified by Ichimura, Terunobu  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  C  
Temperature:  15 (20) C
Light intensity:  7 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (21 D)  
Gene information Whole-genome ( BQMA00000000 ) , Mitochondrial DNA ( LC704714 ) , Plastid DNA ( LC704715 ) , 18S rRNA ( AB363692 ) , 5.8S-ITS2-28S ( AB625555 ) , CpCAB1 ( AB363403 ) , CpHSP70 ( AB363402 ) , CpMADS1 mRNA ( AB091476 ) , CpPI mRNA ( AB012698 ) , ESTs(cDNA) ( AU294770-AU295959 ) , chloroplast phosphoribulokinas ( AB650527 )  
Cell size (min - max) 90 - 110 μm  
Organization Unicellular 
Characteristics Heterothallic: Mating type (−) ; Mating group I B ; Genome decoded strain (Sekimoto et al. 2023)  
Other strain no. Other strain no. : N-31-24  
Remarks Axenic 
Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 53545859626364656826126664319432043214548455045514552 
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Kawai, J., Kanazawa, M., Suzuki, R., Kikuchi, N., Hayakawa, Y., Sekimoto, H. 2022 Highly efficient transformation of the model zygnematophycean alga Closterium peracerosum-strigosum-littorale complex by square-pulse electroporation. New Phytol., 233, 569-578.
Keywords: Closterium; electroporation; evolution; transformation; Zygnematophyceae
Strain(s): 6768 
PubMed: 34605030
DOI: 10.1111/nph.17763

Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 535455646566676826126243194320 
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

Higo, A., Kawashima, T., Borg, M., Zhao, M., López-Vidriero, I., Sakayama, H., Montgomery, S, A., Sekimoto, H., Hackenberg, D., Shimamura, M., Nishiyama, T., Sakakibara, K., Tomita, Y., Togawa, T., Kunimoto, K., Osakabe, A., Suzuki, Y., Yamato, K. T., Ishizaki, K., Nishihama, R., Kohchi, T., Franco-Zorrilla, J. M., Twell, D., Berger, F., Araki, T. 2018 Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants. Nat. Commun., 9, 5283 (article ID).
Strain(s): 6768 
PubMed: 30538242
DOI: 10.1038/s41467-018-07728-3

Sekimoto, H. 2017 Sexual reproduction and sex determination in green algae. J. Plant Res., 130, 423-431.
Keywords: Closterium; Chlamydomonas; Sexual reproduction; Sex determination
Strain(s): 6768 
PubMed: 28188480
DOI: 10.1007/s10265-017-0908-6

Kanda, N., Ichikawa, M., Ono, A., Toyoda, A., Fujiyama, A., Abe, J., Tsuchikane, Y., Nishiyama, T., Sekimoto, H. 2017 CRISPR/Cas9-based knockouts reveal that CpRLP1 is a negative regulator of the sex pheromone PR-IP in the Closterium peracerosum-strigosum-littorale complex. Sci Rep, 7, 17873 (article ID).
Strain(s): 6768 
PubMed: 29259295
DOI: 10.1038/s41598-017-18251-8

Abe, J., Hirano, N., Komiya, A., Kanda, N., Fujiwara, A., Hori, S., Tsuchikane, Y., Sekimoto, H. 2016 Preparation of knockdown transformants of unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Bio-protocol, 6, e1813 (article ID).
Keywords: Knockdown; Alga; Closterium; Charophycean; Transformation
Strain(s): 6768 
DOI: 10.21769/BioProtoc.1813

Abe, J., Hori, S., Sato, M., Sekimoto, H. 2016 Concanavalin A disrupts the release of fibrous material necessary for zygote formation of a unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Front. Plant Sci., 7, 1040 (article ID).
Keywords: charophycean; Closterium; conjugation; gamete; Concanavalin A (Con A); sexual reproduction
Strain(s): 6768 
PubMed: 27468295
DOI: 10.3389/fpls.2016.01040

Hirano, N., Marukawa, Y., Abe, J., Hashiba, S., Ichikawa, M., Tanabe, Y., Ito, M., Nishii, I., Tsuchikane, Y., Sekimoto, H. 2015 A receptor-like kinase, related to cell wall sensor of higher plants, is required for sexual reproduction in the unicellular charophycean alga, Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 56, 1456-1462.
Keywords: Cell wall; Charophycean alga; Closterium peracerosum–strigosum–littorale complex; CrRLK1L-1; Receptor-like kinase; Sexual reproduction
Strain(s): 6768 
PubMed: 25941232
DOI: 10.1093/pcp/pcv065

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 5859606162636465676869702612666 
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Hori, S., Sekimoto, H., Abe, J. 2012 Properties of cell surface carbohydrates in sexual reproduction of the Closterium peracerosum–strigosum–littorale complex (Zygnematophyceae, Charophyta). Phycol. Res., 60, 254-260.
Keywords: cell-cell recognition; Closterium; ConcanavalinA; conjugation; lectin; Lycopersicon esculentum lectin; sexual reproduction
Strain(s): 6768 
DOI: 10.1111/j.1440-1835.2012.00656.x

Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary).
Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone
Strain(s): 58596061626364656768697026145484551 

Abe, J., Hori, S., Tsuchikane, Y., Kitao, N., Kato, M., Shiraiwa, H. 2011 Stable nuclear transformation of the Closterium peracerosum–strigosum–littorale complex. Plant Cell Physiol., 52, 1676-1685.
Strain(s): 6768 
PubMed: 21804095
DOI: 10.1093/pcp/pcr103

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53545859646567686970229261 
PubMed: 27041716
DOI: 10.1111/j.1529-8817.2008.00577.x

Abe, J., Hiwatashi, Y., Ito, M., Hasebe, M., Sekimoto, H. 2008 Expression of exogenous genes under the control of endogenous HSP70 and CAB promoters in the Closterium peracerosum–strigosum–littorale complex. Plant Cell Physiol., 49, 625-632.
Strain(s): 68 
PubMed: 18308758
DOI: 10.1093/pcp/pcn039

Imaizumi, M., Sekimoto, H., Noguchi, T. 2007 Induction of sex pheromone secretion and observations of the secretory pathway in the Closterium peracerosum-strigosum-littorale complex. Plant Sci., 173, 206-212.
Keywords: Closterium peracerosum-strigosum-littorale; Immunoelectron microscopy; Protoplast release-inducing protein; Sex pheromone; Sexual reproduction; Zygote formation
Strain(s): 6768 
DOI: 10.1016/j.plantsci.2007.05.002

Akatsuka, S., Tsuchikane, Y., Fukumoto, R., Fujii, T., Sekimoto, H. 2006 Physiological characterization of the sex pheromone protoplast-release-inducing protein from the Closterium peracerosum-strigosum-littorale complex (Charophyta). Phycol. Res., 54, 116-121.
Keywords: Closterium; conjugation; mucilage; protoplast release; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1111/j.1440-1835.2006.00418.x

Hamada, S., Sekimoto, H., Tanabe, Y., Tsuchikane, Y., Ito, M. 2006 Isolation of myosin XI genes from the Closterium peracerosum-strigosum-littorale complex and analysis of their expression during sexual reproduction. J. Plant Res., 119, 105-113.
Keywords: Closterium; Cytokinesis; Myosin XI; Real-time PCR; Sexual cell division; Sexual reproduction
Strain(s): 656768 
PubMed: 16456621
DOI: 10.1007/s10265-005-0249-8

Tanabe, Y., Hasebe, M., Sekimoto, H., Nishiyama, T., Kitani, M., Henschel, K., Münster, T., Theißen, G., Nozaki, H., Ito, M. 2005 Characterizaion of MADS-box genes in charophycean green alga and its implication for the evolution of MADS-box genes. Proc. Nati. Acad. Sci. U. S. A., 102, 2436-2441.
Keywords: charophyve; land plants; Chara; Coleochaete; Closterium
Strain(s): 6768 
PubMed: 15699346
DOI: 10.1073/pnas.0409860102

Tsuchikane, Y., Fujii, T., Ito, M., Sekimoto, H. 2005 A sex pheromone, protoplast release-inducing protein (PR-IP) inducer, induces sexual cell division and production of PR-IP in Closterium. Plant Cell Physiol., 46, 1472-1476.
Keywords: Closterium; conjugation; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 
PubMed: 15970600
DOI: 10.1093/pcp/pci158

Sekimoto, H. 2004 Molecular characterization of a novel sex pheromone, PR-IP inducer, in Closterium peracerosum-strigosum-littorale complex. Jpn. J. Phycol. (Sôrui), 52, Suppl., 117-122.
Keywords: Closterium; conjugation; gamete mating; sex pheromone, sexual reproduction
Strain(s): 6768 

Tsuchikane, Y., Fukumoto, R., Akatsuka, S. Fujii, T., Sekimoto, H. 2003 Sex pheromones that induce sexual cell division in the Closterium peracerosum-strigosum-littorale complex (Charophyta). J. Phycol., 39, 303-309.
Keywords: Closterium; conjugation; gametangial cell; pheromone; sexual cell division; sexual reproduction
Strain(s): 6768 
DOI: 10.1046/j.1529-8817.2003.02062.x

Sekimoto, H., Tanabe, Y., Takizawa, M., Ito, N., Fukumoto, R., Ito, M. 2003 Expressed sequence tags from the Closterium peracerosum-strigosum-littorale complex, a unicellular charophycean alga, in the sexual reproduction process. DNA Res., 10, 147-153.
Keywords: Closterium; conjugation; EST; sexual reproduction
Strain(s): 6768 
PubMed: 14621293
DOI: 10.1093/dnares/10.4.147

Akatsuka, S., Sekimoto, H., Iwai, H., Fukumoto, R., Fujii, T. 2003 Mucilage secretion regulated by sex pheromones in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 44, 1081-1087.
Keywords: Closterium; mucilage; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1093/pcp/pcg131

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261262263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Sekimoto, H. 2002 Production and secretion of a biologically active Closterium sex pheromone by Saccharomyces cerevisiae. Plant Physiol. Biochem., 40, 789-794.
Keywords: Closterium; conjugation; pheromone; PR-IP; sexual reproduction
Strain(s): 6768 
DOI: 10.1016/S0981-9428(02)01432-8

Sekimoto, H. & Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315.
Strain(s): 64656768 

Sekimoto, H., Fukumoto, R., Dohmae, N., Takio, K. Fujii, T., Kamiya, Y. 1998 Molecular cloning of a novel sex pheromone responsible for the release of a different sex pheromone in Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 39, 1169-1175.
Keywords: Clostreium; conjugation; gamete; mating; sex pheromone; sexual reproduction
Strain(s): 6768 
DOI: 10.1093/oxfordjournals.pcp.a029317

Endo, B., Fujii, T., Kamiya, Y., Sekimoto, H. 1997 Analysis of genomic sequences encoding a sex pheromone from the Closterium peracerosum-strigosum-littorale complex. J. Plant Res., 110, 463-467.
Keywords: cis-element; Closterium; genomic DNA; protoplast-release- inducing protein (PR-IP); sex pheromone; transcription
Strain(s): 6768 
DOI: 10.1007/BF02506807

Nojiri, T., Fujii, T., Sekimoto, H. 1995 Purification and characterization of a novel sex pheromone that induces the release of another sex pheromone during sexual reproduction of the heterothallic Closterium peracerosum-strigosum-littorale complex. Plant Cell Physiol., 36, 79-84.
Keywords: Closterium; conjugation (Closterium); protoplast-release-inducing protein; pheromone
Strain(s): 6768 
DOI: 10.1093/oxfordjournals.pcp.a078747

Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615.
Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation
Strain(s): 64656768 
DOI: 10.1111/j.1529-8817.1995.tb02557.x

Sekimoto, H., Sone, Y., Fujii, T. 1994 Regulation of expression of the genes for a sex pheromone by an inducer of the sex pheromone in the Closterium peracerosum-strigosum-littorale complex. Planta, 193, 137-144.
Keywords: Clostreium complex; inducer (of protoplast-release-inducing protein); pheromone; protoplast-release-inducing protein (PR-IP); sexual reproduction
Strain(s): 6768 
PubMed: 7764622
DOI: 10.1007/BF00191617

Sekimoto, H. & Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese).
Strain(s): 64656768 

Sekimoto, H., Satoh, S., Fujii, T. 1993 Analysis of binding of biotinylated protoplast-release-inducing protein that induces release of gametic protoplasts in the Closterium peracerosum-strigosum-littorale complex. Planta, 189, 468-474.
Keywords: Clostreium; conjugation (Closterium); protoplast-release; inducing protein; pheromone; receptor
Strain(s): 6768 
PubMed: 24178507
DOI: 10.1007/BF00194447

Sekimoto, H. & Fujii, T. 1992 Analysis of gametic protoplast release in the Closterium peracerosum-strigosum-littorale complex (Chlorophyta). J. Phycol., 28, 615-619.
Keywords: biologically active glycoprotein; Closterium peracerosum-strigosum-littorale complex; conjugation; gametic protoplast release
Strain(s): 6768 
DOI: 10.1111/j.0022-3646.1992.00615.x

Sekimoto, H., Satoh, S., Fujii, T. 1992 Biochemical and physiological properties of a gametic protoplast-release-inducing protein in Closterium. Korean J. Phycol., 7, 121-129.
Keywords: Clostreium; conjugation; glycoprotein; protoplast release in conjugation
Strain(s): 6768 

Sekimoto, H. & Fujii, T. 1991 Sexual reproduction of microalgae : its physiological and biochemical study. Cell Science, 7, 562-571 (in Japanese with English title).
Strain(s): 6768 

Sekimoto, H., Satoh, S., Fujii, T. 1990 Biochemical and physiological properties of a protein inducing protoplast release during conjugation in the Closterium peracerosum-strigosum-littorale complex. Planta, 182, 348-354.
Keywords: Clostreium; conjugation (Closterium); glycoprotein; protoplast release in conjugation
Strain(s): 6768 
DOI: 10.1007/BF02411384

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39454651525354555658596061626364656667686970261262266268 

Ichimura, T. 1973 The life cycle and its control in some species of Closterium, with special reference to the biological species problems. Thesis D. Sci., University of Tokyo, 69 pp., 11 tables, 40 figs.
Strain(s): 666768125127174175176177179180181182183185198202262337338450451 

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