Strain Data

Strain number		NIES-64
Phylum		Streptophyta
Class		Zygnematophyceae
Scientific name		Closterium peracerosum-strigosum-littorale complex
Synonym
Former name
Common name		Desmid
Locality (Date of collection)		Lake Kasumigaura, Tamatsukuri, Ibaraki, Japan (1974-11-**)
Latitude / Longitude		36.0428477398 / 140.373233439
Habitat (Isolation source)		Freshwater (Lake water)
History		< Watanabe, Makoto M.
Isolator (Date of isolation)		Watanabe, Makoto M. (1974-11-**)
Identified by		Watanabe, Makoto M.
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 15 (20) C Light intensity: 7 (10-17) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (21 D)
Gene information		18S rRNA ( AB363686 ) , 5.8S-ITS2-28S ( AB625544 )
Cell size (min - max)		10 - 250 μm
Organization		Unicellular
Characteristics		Heterothallic: Mating type (−) ; Mating group II B
Other strain no.		Other strain no. : KAS-4-29
Remarks		Axenic
Movie

Reference
Kawaguchi, Y. W. & Yamamichi, M. 2025 A single episode of sexual reproduction can produce large variation in population growth rates under dual stressors. J. Evol. Biol., Keywords: Adaptation; evolutionary rescue; multiple stressors; population biology; sex; transgressive segregation Strain(s): 64, 65 PubMed: 40241314 DOI: 10.1093/jeb/voaf041 Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID). Keywords: gene duplication; dosage compensation; copy number variation; green algae Strain(s): 53, 54, 58, 59, 62, 63, 64, 65, 68, 261, 2666, 4319, 4320, 4321, 4548, 4550, 4551, 4552 PubMed: 37348049 DOI: 10.1093/gbe/evad115 Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746. Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation Strain(s): 53, 54, 55, 64, 65, 66, 67, 68, 261, 4319, 4320 PubMed: 29948484 DOI: 10.1007/s10265-018-1043-8 Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary). Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551 Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9. Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666 PubMed: 21858438 DOI: 10.1007/s00497-011-0174-z Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203. Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261 PubMed: 27041716 DOI: 10.1111/j.1529-8817.2008.00577.x Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352. Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction Strain(s): 64, 65, 228, 229 DOI: 10.1007/PL00013941 Sekimoto, H. & Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315. Strain(s): 64, 65, 67, 68 Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615. Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation Strain(s): 64, 65, 67, 68 DOI: 10.1111/j.1529-8817.1995.tb02557.x Sekimoto, H. & Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese). Strain(s): 64, 65, 67, 68 Nakayama, T., Ishii, E., Kanazawa, H. 1987 The study on the isolation and culture of the protoplast from heterothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 14, 95-114. Strain(s): 64, 65 Kato, A., Ohmura, K., Kanazawa, H., Sasaki, K. 1983 Natural and artificial production of protoplasts from heterothallic and homothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 7-15. Strain(s): 64, 65 Kato, A. & Sasaki, K. 1983 Effects of tunica-mycin on sexual reproduction in heterothallic strains of Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 1-6. Strain(s): 64, 65 Watanabe, M. M. & Ichimura, T. 1982 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. IV. Hybrid breakdown between two closely related groups, Group II-A and Group II-B. Bot. Mag. Tokyo, 95, 241-247. Keywords: Closterium; fertility; hybrid breakdown; post-zygotic isolating mechanism; viability Strain(s): 51, 52, 53, 64, 65 DOI: 10.1007/BF02488536 Kato, A., Obokata, J., Sasaki, K. 1981 Mating type interaction in Closterium peracerosum-strigosum-littorale: mating induced protoplast release. Plant Cell Physiol., 22, 1215-1222. Keywords: Closterium peracerosum-strigosum-littorale; conjugation; disruped cell; mating type interaction; protoplast release Strain(s): 64, 65 DOI: 10.1093/oxfordjournals.pcp.a076273 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 DOI: 10.1007/BF02489099 Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10. Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs. Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268

Reference

Kawaguchi, Y. W. & Yamamichi, M. 2025 A single episode of sexual reproduction can produce large variation in population growth rates under dual stressors. J. Evol. Biol.,
Keywords: Adaptation; evolutionary rescue; multiple stressors; population biology; sex; transgressive segregation
Strain(s): 64, 65
PubMed: 40241314
DOI: 10.1093/jeb/voaf041

Kawaguchi, Y. W., Tsuchikane, Y., Tanaka, K., Taji, T., Suzuki, Y., Toyoda, A., Ito, M., Watano, Y., Nishiyama, T., Sekimoto, H., Tsuchimatsu, T. 2023 Extensive copy number variation explains genome size variation in the unicellular Zygnematophycean alga, Closterium peracerosum–strigosum–littorale complex. Genome Biol. Evol., 15, evad115 (article ID).
Keywords: gene duplication; dosage compensation; copy number variation; green algae
Strain(s): 53, 54, 58, 59, 62, 63, 64, 65, 68, 261, 2666, 4319, 4320, 4321, 4548, 4550, 4551, 4552
PubMed: 37348049
DOI: 10.1093/gbe/evad115

Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 53, 54, 55, 64, 65, 66, 67, 68, 261, 4319, 4320
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

Sekimoto, H. 2012 Speciation and reproductive isolation in unicellular charophycean alga, Closterium peraceroum-strigosum-littorale complex. IFO Res. Commun., 26, 71-85 (in Japanese with English summary).
Keywords: Closterium: heterothallism; homothallism; reproductive isolation; sex pheromone
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 4548, 4551

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261
PubMed: 27041716
DOI: 10.1111/j.1529-8817.2008.00577.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 263, 266, 267, 268, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 312, 313, 329, 334, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352.
Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction
Strain(s): 64, 65, 228, 229
DOI: 10.1007/PL00013941

Sekimoto, H. & Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315.
Strain(s): 64, 65, 67, 68

Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615.
Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation
Strain(s): 64, 65, 67, 68
DOI: 10.1111/j.1529-8817.1995.tb02557.x

Sekimoto, H. & Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese).
Strain(s): 64, 65, 67, 68

Nakayama, T., Ishii, E., Kanazawa, H. 1987 The study on the isolation and culture of the protoplast from heterothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 14, 95-114.
Strain(s): 64, 65

Kato, A., Ohmura, K., Kanazawa, H., Sasaki, K. 1983 Natural and artificial production of protoplasts from heterothallic and homothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 7-15.
Strain(s): 64, 65

Kato, A. & Sasaki, K. 1983 Effects of tunica-mycin on sexual reproduction in heterothallic strains of Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 1-6.
Strain(s): 64, 65

Watanabe, M. M. & Ichimura, T. 1982 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. IV. Hybrid breakdown between two closely related groups, Group II-A and Group II-B. Bot. Mag. Tokyo, 95, 241-247.
Keywords: Closterium; fertility; hybrid breakdown; post-zygotic isolating mechanism; viability
Strain(s): 51, 52, 53, 64, 65
DOI: 10.1007/BF02488536

Kato, A., Obokata, J., Sasaki, K. 1981 Mating type interaction in Closterium peracerosum-strigosum-littorale: mating induced protoplast release. Plant Cell Physiol., 22, 1215-1222.
Keywords: Closterium peracerosum-strigosum-littorale; conjugation; disruped cell; mating type interaction; protoplast release
Strain(s): 64, 65
DOI: 10.1093/oxfordjournals.pcp.a076273

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261
DOI: 10.1007/BF02489099

Watanabe, M. M. & Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10.
Strain(s): 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 266, 268

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