Strain Data

Strain number		NIES-64
Phylum		Streptophyta
Class		Charophyceae
Scientific name		Closterium peracerosum-strigosum-littorale complex
Synonym
Former name
Common name		Desmid
Locality (Date of collection)		Lake Kasumigaura Tamatsukuri Ibaraki Japan (1974-11-**)
Latitude / Longitude		36.0428477398 / 140.373233439
Habitat (Isolation source)		Freshwater (Lake water)
History		< Watanabe, Makoto M.
Isolator (Date of isolation)		Watanabe, Makoto M. (1974-11-**)
Identified by		Watanabe, Makoto M.
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: C Temperature: 15 (20) C Light intensity: 7 (10-17) µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 3 M (21 D)
Gene information		18S rRNA ( AB363686 ) , 5.8S-ITS2-28S ( AB625544 )
Cell size (min - max)		10 - 250 μm
Organization		Unicellular
Characteristics		Heterothallic: Mating type (−) ; Mating group II B
Other strain no.		Other strain no. : KAS-4-29
Remarks		Axenic
Movie

Reference
Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746. Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation Strain(s): 53, 54, 55, 64, 65, 66, 67, 68, 261, 262 PubMed: 29948484 DOI: 10.1007/s10265-018-1043-8 Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9. Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666 PubMed: 21858438 DOI: 10.1007/s00497-011-0174-z Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203. Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261 PubMed: 27041716 DOI: 10.1111/j.1529-8817.2008.00577.x Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55. Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729 Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352. Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction Strain(s): 64, 65, 228, 229 DOI: 10.1007/PL00013941 Sekimoto, H., Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315. Strain(s): 64, 65, 67, 68 Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615. Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation Strain(s): 64, 65, 67, 68 DOI: 10.1111/j.1529-8817.1995.tb02557.x Sekimoto, H., Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese without English title). Strain(s): 64, 65, 67, 68 Nakayama, T., Ishii, E., Kanazawa, H. 1987 The study on the isolation and culture of the protoplast from heterothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 14, 95-114. Strain(s): 64, 65 Kato, A., Ohmura, K., Kanazawa, H., Sasaki, K. 1983 Natural and artificial production of protoplasts from heterothallic and homothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 7-15. Strain(s): 64, 65 Kato, A., Sasaki, K. 1983 Effects of tunica-mycin on sexual reproduction in heterothallic strains of Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 1-6. Strain(s): 64, 65 Watanabe, M. M., Ichimura, T. 1982 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. IV. Hybrid breakdown between two closely related groups, Group II-A and Group II-B. Bot. Mag. Tokyo, 95, 241-247. Keywords: Closterium; fertility; hybrid breakdown; post-zygotic isolating mechanism; viability Strain(s): 51, 52, 53, 64, 65 DOI: 10.1007/BF02488536 Kato, A., Obokata, J., Sasaki, K. 1981 Mating type interaction in Closterium peracerosum-strigosum-littorale: mating induced protoplast release. Plant Cell Physiol., 22, 1215-1222. Keywords: Closterium peracerosum-strigosum-littorale; conjugation; disruped cell; mating type interaction; protoplast release Strain(s): 64, 65 DOI: 10.1093/oxfordjournals.pcp.a076273 Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10. Strain(s): 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24. Strain(s): 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261 DOI: 10.1007/BF02489099 Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs. Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 262, 266, 268

Reference

Tsuchikane, Y., Kobayashi, H., Kato, M., Watanabe, J., Wu, J. T., Sekimoto, H. 2018 Identification of a new mating group and reproductive isolation in the Closterium peracerosum–strigosum–littorale complex. J. Plant Res., 131, 735–746.
Keywords: Alga; Closterium; Mating group; Reproductive isolation; Sex pheromone; Speciation
Strain(s): 53, 54, 55, 64, 65, 66, 67, 68, 261, 262
PubMed: 29948484
DOI: 10.1007/s10265-018-1043-8

Tsuchikane, Y., Tsuchiya, M., Hindák, F., Nozaki, H., Sekimoto, H. 2012 Zygospore formation between homothallic and heterothallic strains of Closterium. Sex. Plant Reprod., 25, 1-9.
Keywords: Closterium; Conjugation; Homotallism; Heterothallism: Dexual reproduction; Zygospore
Strain(s): 58, 59, 60, 61, 62, 63, 64, 65, 67, 68, 69, 70, 261, 2666
PubMed: 21858438
DOI: 10.1007/s00497-011-0174-z

Tsuchikane, Y., Ito, M., Sekimoto, H. 2008 Reproductive isolation by sex pheromones in the Closterium peracerosum-strigosum-littorale complex (Zygnematales, Charophyceae). J. Phycol., 44, 1197-1203.
Keywords: Closterium; conjugation; pheromone; reproductive isolation; sexual reproduction
Strain(s): 53, 54, 58, 59, 64, 65, 67, 68, 69, 70, 229, 261
PubMed: 27041716
DOI: 10.1111/j.1529-8817.2008.00577.x

Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 19, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 44, 45, 46, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 73, 74, 75, 76, 77, 78, 79, 80, 81, 87, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 119, 120, 122, 123, 124, 125, 127, 128, 129, 130, 131, 132, 133, 134, 135, 137, 138, 139, 144, 147, 148, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178, 179, 180, 181, 182, 183, 185, 186, 187, 188, 189, 191, 192, 193, 194, 195, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 224, 226, 227, 228, 229, 230, 231, 232, 241, 242, 243, 244, 245, 246, 248, 257, 259, 261, 262, 263, 266, 267, 268, 271, 287, 288, 289, 290, 294, 295, 297, 298, 299, 300, 301, 302, 303, 305, 306, 308, 309, 310, 311, 312, 313, 329, 334, 336, 337, 338, 339, 340, 341, 342, 349, 351, 359, 360, 361, 362, 375, 378, 379, 380, 382, 384, 385, 390, 394, 396, 397, 398, 415, 416, 418, 419, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 436, 437, 438, 439, 440, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458, 459, 460, 464, 468, 469, 474, 478, 479, 480, 481, 503, 504, 505, 506, 507, 509, 510, 512, 514, 515, 522, 523, 524, 527, 528, 529, 530, 531, 532, 536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584, 585, 592, 593, 594, 595, 596, 597, 598, 604, 610, 628, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, 661, 662, 663, 664, 665, 666, 667, 672, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726, 727, 728, 729

Sekimoto, H. 2000 Intercellular communication during sexual reproduction of Closterium (Conjugatophyceae). J. Plant Res., 113, 343-352.
Keywords: chemotaxis; Closterium; conjugation; mating; sex pheromone; sexual reproduction
Strain(s): 64, 65, 228, 229
DOI: 10.1007/PL00013941

Sekimoto, H., Fukumoto, R. 1999 Sex pheromones in Closterium. Chem. Regul. Plant, 34, 305-315.
Strain(s): 64, 65, 67, 68

Sekimoto, H., Sone, Y., Fujii, T. 1995 Biochemical, physiological, and molecular analysis of sexual isolation in the species complex Closterium peracerosum-strigosum-littorale (Chlorophyta). J. Phycol., 31, 611-615.
Keywords: Chlorophyta; Closterium peracerosum-strigosum-littorale complex; conjugation; pheromone; plotoplast-release-inducing protein (PR-IP); sexual isolation
Strain(s): 64, 65, 67, 68
DOI: 10.1111/j.1529-8817.1995.tb02557.x

Sekimoto, H., Fujii, T. 1993 Cell-cell interaction involved in the sexual reproduction of Closterium [Mikadukimo no yûsei seisyoku ni kakawaru saibôkan jôhô kôkan]. Iden, 47 (1), 43-48 (in Japanese without English title).
Strain(s): 64, 65, 67, 68

Nakayama, T., Ishii, E., Kanazawa, H. 1987 The study on the isolation and culture of the protoplast from heterothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 14, 95-114.
Strain(s): 64, 65

Kato, A., Ohmura, K., Kanazawa, H., Sasaki, K. 1983 Natural and artificial production of protoplasts from heterothallic and homothallic Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 7-15.
Strain(s): 64, 65

Kato, A., Sasaki, K. 1983 Effects of tunica-mycin on sexual reproduction in heterothallic strains of Closterium. J. Fac. Sci., Hokkaido Univ., Ser. V (Bot.), 13, 1-6.
Strain(s): 64, 65

Watanabe, M. M., Ichimura, T. 1982 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. IV. Hybrid breakdown between two closely related groups, Group II-A and Group II-B. Bot. Mag. Tokyo, 95, 241-247.
Keywords: Closterium; fertility; hybrid breakdown; post-zygotic isolating mechanism; viability
Strain(s): 51, 52, 53, 64, 65
DOI: 10.1007/BF02488536

Kato, A., Obokata, J., Sasaki, K. 1981 Mating type interaction in Closterium peracerosum-strigosum-littorale: mating induced protoplast release. Plant Cell Physiol., 22, 1215-1222.
Keywords: Closterium peracerosum-strigosum-littorale; conjugation; disruped cell; mating type interaction; protoplast release
Strain(s): 64, 65
DOI: 10.1093/oxfordjournals.pcp.a076273

Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. II. Reproductive isolation and morphological variation among several populations from the Northern Kanto area in Japan. Bot. Mag. Tokyo, 91, 1-10.
Strain(s): 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261

Watanabe, M. M., Ichimura, T. 1978 Biosystematic studies of the Closterium peracerosum-strigosum-littorale complex. III. Degrees of sexual isolation among the three population groups from the Northern Kanto area. Bot. Mag. Tokyo, 91, 11-24.
Strain(s): 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 69, 70, 261
DOI: 10.1007/BF02489099

Watanabe, M. M. 1977 Biosystematics in Closterium of sexual unicellular green algae and Calothrix and Spirulina of asexual filamentous blue-green algae, with special reference to the analyses of natural populations. Thesis D. Sci., Hokkaido University, 114 pp., 28 tables, 54 figs.
Strain(s): 39, 45, 46, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 261, 262, 266, 268

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