Strain number NIES-168  
Phylum Streptophyta  
Class Zygnematophyceae  
Scientific name Closterium calosporum Wittrock var. galiciense Gutwinski  
Synonym  
Former name  
Common name Desmid  
Locality (Date of collection) Hoshidate, Iriomote Isl., Okinawa, Japan (1973-03-**)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater (Paddy soil)  
History < Watanabe, Masayuki  
Isolator (Date of isolation) Ichimura, Terunobu  
Identified by Watanabe, Masayuki  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  CA  
Temperature:  20 (20) C
Light intensity:  7-8 (10-17) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  3 M (1 M)  
Gene information  
Cell size (min - max)  
Organization Unicellular 
Characteristics Heterothallic: Mating type (−) ; Crosses with NIES-165  
Other strain no. Other strain no. : R-11-5  
Remarks Axenic 
Movie  
Reference
Mori, F., Erata, M., Watanabe, M. M. 2002 Cryopreservation of cyanobacteria and green algae in the NIES-Collection. Microbiol. Cult. Coll., 18, 45-55.
Keywords: cryopreservation; cyanobacteria; green algae; FDA staining method; NIES-Collection
Strain(s): 192122232425262728293031323334353637383940414445465051525354555658596061626364656667686970737475767778798081878990919293949596979899100101102103104105106107108109110111112119120122123125127128129130131132133134135137138139144147151152153154155156157158159160162163164165166167168170171172173174175176177178179180181182183185186187188189191192193194197198199200201202203204205206207208209210211212213214215216217224226227228229230231232241242243244245246248257259261263266267268287288289290294295297298299300301302303305306308309310312313329334337338339340341342349351359360361362375378379380382384385390394396397398415416418419421422423424425426427428429430431432433434436437438439440446447448449450451452453454455456457458459460464468469474478479480481503504505506507509510512514515522523524527528529530531532536537538539540541542543544545546564565566567568569570571572573574575576577578579580581582583584585592593594595596597598604610628630631632633634635636637638639640641642643644645646647648649650651652653654655656657658659660661662663664665666667672685686687688689690691692693694717718719720721722723724725726727728729 

Watanabe, M. 1979 A taxonomic study of the Closterium calosporum complex (2). Bull. Natn. Sci. Mus., Ser. B (Bot.), 5, 1-23, pls. 9-11.
Strain(s): 163164165168186187188189191192194197339340341 

Watanabe, M. 1978 A taxonomic study of the Closterium calosporum complex (1). Bull. Natn. Sci. Mus., Ser. B (Bot.), 4, 133-154, pls. 1-8.
Strain(s): 163164165168171186187188189191192193194197339341 

Ichimura, T. & Watanabe, M. 1974 The Closterium calosporum complex from the Ryukyu Islands - Variation and taxonomical problems. Mem. Natn. Sci. Mus. Tokyo, 7, 89-102, pls. 13-14.
Strain(s): 163164165168186187194339340341 

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