Strain number NIES-1628  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Nitella megaspora (J.Gloves) Sakayama  
Synonym Nitella pseudoflabellata A.Braun f. megaspora (J.Groves) R.D.Wood  
Former name  
Common name Stonewort  
Locality (Date of collection) Junsai-numa Pond Sanda, Kashita Hyogo Japan (2002-09-12)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < Sakayama, Hidetoshi  
Isolator (Date of isolation)  
Identified by Sakayama, Hidetoshi  
State of strain Subculture; Non-axenic  
Culture condition
(Preculture condition)
Medium:  mSWC-2SWCN-3  
Temperature:  20 C
Light intensity:  16-20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S-ITS1-5.8S-ITS2-28S ( AB169944 ) , atpB ( AB169962 KJ395818 ) , psaB ( AB191768 ) , rbcL ( AB169970 KJ395935 )  
Cell size (min - max)  
Organization  
Characteristics Monoecious ; CR+EN  
Other strain no. Other strain no. : CH-114; S073  
Remarks  
Movie  
Reference
Sakayama, H. 2008 Taxonomy of Nitella (Charales, Charophyceae) based on comparative morphology of oospores and multiple DNA marker phylogeny using cultured material. Phycol. Res., 56, 202-215.
Keywords: Charales; Charophyceae; chloroplast DNA; morphology; Nitella; nuclear DNA; oospores; scanning electron microscopy; taxonomy; Tieffallenia
Strain(s): 1611161516281634170417051706 
DOI: 10.1111/j.1440-1835.2008.00502.x

Sakayama, H., Miyaji, K., Nagumo, T., Kato, M., Hara Y., Nozaki, H. 2005 Taxonomic re-examination of 17 species of Nitella subgenus Tieffallenia (Charales, Charophyceae) based on internal morphology of the oospore wall and multiple DNA marker sequences. J. Phycol., 41, 195-211.
Keywords: Charales; Charophyceae; chloroplast DNA; morphology; Nitella; nuclear DNA; oospores; scanning electron microscopy; taxonomy; Tieffallenia
Strain(s): 16151628 
DOI: 10.1111/j.1529-8817.2005.04133.x

Sakayama, H., Hara, Y., Arai, S., Sato, H., Nozaki, H. 2004 Phylogenetic analyses of Nitella subgenus Tieffallenia (Charales, Charophyceae) using nuclear ribosomal DNA internal transcribed spacer sequences. Phycologia, 43, 672-681.
Strain(s): 16151628 
DOI: 10.2216/i0031-8884-43-6-672.1

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