Strain number NIES-1611  
Phylum Streptophyta  
Class Charophyceae  
Scientific name Nitella flexilis (L.) C.Agardh  
Former name  
Common name Stonewort  
Locality (Date of collection) Oh-ike Pond, Yoshii, Kato, Hyogo, Japan (2001-06-02)  
Latitude / Longitude  
Habitat (Isolation source) Freshwater  
History < Sakayama, Hidetoshi  
Isolator (Date of isolation) Sakayama, Hidetoshi  
Identified by Sakayama, Hidetoshi  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  mSWC-2SWCN-1SWCN-2SWCN-3  
Temperature:  20 C
Light intensity:  16-20 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M  
Gene information  
Cell size (min - max)  
Characteristics Monoecious ; CR+EN  
Other strain no. Other strain no. : CH-62: S069  
Su, D., Yang, L., Shi, X., Ma, X., Zhou, X., Hedges, S. B., Zhong, B. 2021 Large-scale phylogenomic analyses reveal the monophyly of bryophytes and Neoproterozoic origin of land plants. Mol. Biol. Evol., 38, 3332–3344.
Keywords: Land plants; bryophytes; phylogeny; timescale; maximum bounds
Strain(s): 160616111638 
PubMed: 33871608
DOI: 10.1093/molbev/msab106

Sakayama, H. 2008 Taxonomy of Nitella (Charales, Charophyceae) based on comparative morphology of oospores and multiple DNA marker phylogeny using cultured material. Phycol. Res., 56, 202-215.
Keywords: Charales; Charophyceae; chloroplast DNA; morphology; Nitella; nuclear DNA; oospores; scanning electron microscopy; taxonomy; Tieffallenia
Strain(s): 161116281634170417051706 
DOI: 10.1111/j.1440-1835.2008.00502.x

Nozaki, H., Mori, T., Misumi, O., Matsunaga, S., Kuroiwa, T. 2006 Males evolved from the dominant isogametic mating type. Curr. Biol., 16, R1018-R1020.
Strain(s): 16111614221222132214 
PubMed: 17174904
DOI: 10.1016/j.cub.2006.11.019

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