Strain Data

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Strain number		NIES-274
Phylum		Cryptophyta
Class		Cryptophyceae
Scientific name		Cryptomonas ovata Ehrenberg
Synonym
Former name
Common name
Locality (Date of collection)		Tsuchiura, Ibaraki, Japan (1982-10-28)
Latitude / Longitude		36.098284 / 140.194777
Habitat (Isolation source)		Freshwater (Pond water)
History		< Ishimitsu, Mayumi
Isolator (Date of isolation)		Ishimitsu, Mayumi (1982-10-28)
Identified by		Ishimitsu, Mayumi
State of strain		Subculture; Unialgal; Clonal; Axenic[2017 Dec]
Culture condition (Preculture condition)		Medium: VT Temperature: 15 C Light intensity: 20 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		16S rRNA ( AB073109 ) , 18S rRNA ( AB240952 ) , atpA ( AB280580 ) , CO1 ( AB009419 ) , psbO ( AB545777 ) , rbcL ( AB280610 )
Cell size (min - max)		- 45 μm
Organization		Unicellular; Flagellate
Characteristics
Other strain no.		Other strain no. : #00046
Remarks		Axenic
Movie

Reference
Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62. Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392 Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID). Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778 DOI: 10.1016/j.algal.2021.102191 Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910. Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1868, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775 PubMed: 30809012 DOI: 10.1038/s41396-019-0377-0 Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29. Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691 Hoef-Emden, K. 2012 Pitfalls of establishing DNA barcoding systems in protists: the cryptophyceae as a test case. PLoS One, 7, e43652 (article ID). Strain(s): 274, 706, 708, 711, 712, 713, 714, 1004, 1370 PubMed: 22970104 DOI: 10.1371/journal.pone.0043652 Yokoyama, A., Takahashi, F., Kataoka, H., Hara, Y., Nozaki, H. 2011 Evolutionary analysis of the nuclear-encoded photosynthetic gene psbO from tertiary plastid-containing algae in Dinophyta. J. Phycol., 47, 407-414. Keywords: diatoms; Dinophyta; endosymbiosis; Haptophyta; N-terminal region; phylogeny; plastid; psbO genes Strain(s): 274, 712 PubMed: 27021871 DOI: 10.1111/j.1529-8817.2011.00961.x Tanifuji, G., Onodera, N., Hara, Y. 2010 Nucleomorph genome diversity and its phylogenetic implications in cryptomonad algae. Phycol. Res., 58, 230-237. Keywords: cryptomonads; endosymbiosis; evolution; genome reduction; nucleomorph; phylogeny Strain(s): 274, 277, 280, 697, 699, 701, 702, 704, 708, 712, 715, 765 DOI: 10.1111/j.1440-1835.2010.00580.x Hamana, K. & Niitsu, M. 2006 Cellular polyamines of lower eukaryotes belonging to the phyla Glaucophyta, Rhodophyta, Cryptophyta, Haptophyta and Percolozoa. J. Gen. Appl. Microbiol., 52, 235-240. Keywords: Cryptophyta; Glaucophyta; Haptophyta; Perclozoa; polyamine; Rhodophyta Strain(s): 8, 274, 276, 277, 282, 353, 388, 699, 706, 715, 741, 763, 764, 1016, 1017, 1032, 1036, 1332, 1441, 1456, 1457, 1462, 1463, 1467, 1473, 3377 PubMed: 17116972 DOI: 10.2323/jgam.52.235 Hoef-Emden, K., Melkonian, M. 2003 Revision of the genus Cryptomonas (Cryptophyceae): a combination of molecular phylogeny and morphology provides insights into a long-hidden dimorphism. Protist, 154, 371-409. Strain(s): 274, 276, 279 PubMed: 14658496 DOI: 10.1078/143446103322454130 Takishita, K., Koike, K., Maruyama, T., Ogata, T. 2002 Molecular evidence for plastid robbery (kleptoplastidy) in Dinophysis, a dinoflagellate causing diarrhetic shellfish poisoning. Protist, 153, 293-302. Strain(s): 274, 715 PubMed: 12389818 DOI: 10.1078/1434-4610-00106 Inagaki, Y., Ehara, M., Watanabe, K. I., Hayashi-Ishimaru, Y., Ohama, T. 1998 Directionally evolving genetic code: the UGA codon from stop to tryptophan in mitochondria. J. Mol. Evol., 47, 378-384. Keywords: COXI phylogenetic tree; UGA codon; deviant genetic code; directional codon reassignment Strain(s): 12, 227, 274 PubMed: 9767683 DOI: 10.1007/PL00006395 Ishimitsu, M. & Chihara, M. 1984 Four species of Cryptomonas (Class Cryptophyceae) in Japan. J. Jpn. Bot., 59, 161-169. Strain(s): 274, 275, 276, 277, 278, 279, 280, 281, 282, 344, 345, 346, 347, 348

Reference

Hamana, K., Furuchi, T., Hayashi, H., Niitsu, M. 2022 Additional polyamine analysis of algal chlorarachniophytes, euglenophytes, haptophytes, cryptophytes, dinoflagellates, chromerids and heterokontophytes. —Polyamine analysis of algae IV— Microb. Resour. Syst., 38, 51-62.
Keywords: alga; chlorarachniophyte; chromerid; dinoflagellate; polyamine
Strain(s): 12, 47, 48, 253, 274, 276, 277, 282, 286, 304, 331, 381, 387, 463, 623, 624, 699, 706, 715, 741, 1443, 2145, 2146, 2149, 2351, 2433, 2535, 2584, 2588, 2635, 2677, 2716, 2728, 2860, 3576, 3745, 4060, 4109, 4280, 4391, 4392

Al-Azab, A. J. S., Widyaningrum, D., Hirakawa, H., Hayashi, Y., Tanaka, S., Ohama, T. 2021 A resin cyanoacrylate nanoparticle as an acute cell death inducer to broad spectrum of microalgae. Algal Res., 54, 102191 (article ID).
Keywords: Cell death; Coccolith; Non-green algae; ROS; Poly (isobutylcyanoacrylate) nanoparticles
Strain(s): 1, 5, 7, 15, 274, 331, 534, 590, 615, 699, 712, 900, 1017, 1308, 1310, 2764, 2778
DOI: 10.1016/j.algal.2021.102191

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8, 254, 274, 286, 324, 331, 353, 381, 494, 623, 624, 997, 1012, 1013, 1015, 1320, 1330, 1333, 1334, 1335, 1372, 1377, 1388, 1390, 1396, 1397, 1408, 1438, 1439, 1441, 1868, 1964, 2008, 2142, 2144, 2149, 2300, 2305, 2325, 2433, 2498, 2502, 2566, 2584, 2586, 2589, 2590, 2677, 2878, 3356, 3374, 3775
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Mitani, E., Nakayama, F., Matsuwaki, I., Ichi, I., Kawabata, A., Kawachi, M., Kato, M. 2017 Fatty acid composition profiles of 235 strains of three microalgal divisions within the NIES Microbial Culture Collection. Microb. Resour. Syst., 33, 19-29.
Keywords: Cryptophyta; docosahexaenoic acid; eicosapentaenoic acid; fatty acid; Haptophyta; Heterokontophyta; microalgae
Strain(s): 1, 8, 9, 14, 15, 17, 71, 115, 223, 225, 233, 234, 265, 274, 275, 276, 277, 278, 279, 280, 281, 282, 284, 293, 323, 324, 330, 333, 344, 345, 346, 347, 348, 350, 353, 372, 377, 388, 391, 395, 407, 408, 409, 413, 414, 417, 461, 462, 466, 487, 534, 548, 553, 556, 557, 558, 559, 560, 562, 587, 588, 589, 590, 603, 605, 622, 623, 695, 696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712, 713, 714, 715, 716, 741, 765, 766, 767, 805, 837, 997, 1001, 1002, 1003, 1004, 1005, 1006, 1007, 1009, 1011, 1016, 1017, 1044, 1045, 1046, 1047, 1302, 1303, 1324, 1327, 1330, 1339, 1340, 1349, 1353, 1370, 1375, 1376, 1379, 1383, 1384, 1385, 1386, 1387, 1391, 1392, 1393, 1395, 1398, 1399, 1400, 1401, 1699, 1700, 1730, 1813, 1815, 1816, 1826, 1827, 1831, 1862, 1863, 1864, 1865, 1874, 1963, 1964, 1965, 1974, 1975, 1976, 2142, 2143, 2144, 2145, 2147, 2148, 2300, 2331, 2332, 2351, 2363, 2364, 2365, 2369, 2370, 2376, 2506, 2533, 2534, 2535, 2536, 2537, 2590, 2633, 2668, 2689, 2690, 2691, 2693, 2694, 2696, 2697, 2707, 2716, 2717, 2718, 2720, 2722, 2723, 2725, 2726, 2729, 2730, 2731, 2732, 2770, 2771, 2772, 2773, 2839, 2840, 2841, 2842, 2843, 2844, 2859, 2872, 2878, 2890, 2899, 3391, 3689, 3690, 3691

Hoef-Emden, K. 2012 Pitfalls of establishing DNA barcoding systems in protists: the cryptophyceae as a test case. PLoS One, 7, e43652 (article ID).
Strain(s): 274, 706, 708, 711, 712, 713, 714, 1004, 1370
PubMed: 22970104
DOI: 10.1371/journal.pone.0043652

Yokoyama, A., Takahashi, F., Kataoka, H., Hara, Y., Nozaki, H. 2011 Evolutionary analysis of the nuclear-encoded photosynthetic gene psbO from tertiary plastid-containing algae in Dinophyta. J. Phycol., 47, 407-414.
Keywords: diatoms; Dinophyta; endosymbiosis; Haptophyta; N-terminal region; phylogeny; plastid; psbO genes
Strain(s): 274, 712
PubMed: 27021871
DOI: 10.1111/j.1529-8817.2011.00961.x

Tanifuji, G., Onodera, N., Hara, Y. 2010 Nucleomorph genome diversity and its phylogenetic implications in cryptomonad algae. Phycol. Res., 58, 230-237.
Keywords: cryptomonads; endosymbiosis; evolution; genome reduction; nucleomorph; phylogeny
Strain(s): 274, 277, 280, 697, 699, 701, 702, 704, 708, 712, 715, 765
DOI: 10.1111/j.1440-1835.2010.00580.x

Hamana, K. & Niitsu, M. 2006 Cellular polyamines of lower eukaryotes belonging to the phyla Glaucophyta, Rhodophyta, Cryptophyta, Haptophyta and Percolozoa. J. Gen. Appl. Microbiol., 52, 235-240.
Keywords: Cryptophyta; Glaucophyta; Haptophyta; Perclozoa; polyamine; Rhodophyta
Strain(s): 8, 274, 276, 277, 282, 353, 388, 699, 706, 715, 741, 763, 764, 1016, 1017, 1032, 1036, 1332, 1441, 1456, 1457, 1462, 1463, 1467, 1473, 3377
PubMed: 17116972
DOI: 10.2323/jgam.52.235

Hoef-Emden, K., Melkonian, M. 2003 Revision of the genus Cryptomonas (Cryptophyceae): a combination of molecular phylogeny and morphology provides insights into a long-hidden dimorphism. Protist, 154, 371-409.
Strain(s): 274, 276, 279
PubMed: 14658496
DOI: 10.1078/143446103322454130

Takishita, K., Koike, K., Maruyama, T., Ogata, T. 2002 Molecular evidence for plastid robbery (kleptoplastidy) in Dinophysis, a dinoflagellate causing diarrhetic shellfish poisoning. Protist, 153, 293-302.
Strain(s): 274, 715
PubMed: 12389818
DOI: 10.1078/1434-4610-00106

Inagaki, Y., Ehara, M., Watanabe, K. I., Hayashi-Ishimaru, Y., Ohama, T. 1998 Directionally evolving genetic code: the UGA codon from stop to tryptophan in mitochondria. J. Mol. Evol., 47, 378-384.
Keywords: COXI phylogenetic tree; UGA codon; deviant genetic code; directional codon reassignment
Strain(s): 12, 227, 274
PubMed: 9767683
DOI: 10.1007/PL00006395

Ishimitsu, M. & Chihara, M. 1984 Four species of Cryptomonas (Class Cryptophyceae) in Japan. J. Jpn. Bot., 59, 161-169.
Strain(s): 274, 275, 276, 277, 278, 279, 280, 281, 282, 344, 345, 346, 347, 348

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