Strain Data

Strain number		NIES-161
Phylum		Heterokontophyta
Class		Raphidophyceae
Scientific name		Chattonella marina (Subrahmanyan) Y.Hara & Chihara var. antiqua (Hada) Demura & Kawachi
Synonym		Hemieutreptia antiqua Hada 1974; Chattonella antiqua (Hada) Ono 1980
Former name		Chattonella antiqua (Hada) Ono
Common name
Locality (Date of collection)		Hiroshima Bay, Hiroshima, Japan
Latitude / Longitude
Habitat (Isolation source)		Marine
History		< Takayama, Haruyoshi
Isolator (Date of isolation)
Identified by
State of strain		Subculture; Unialgal; Clonal; Non-axenic[2018 Feb]
Culture condition (Preculture condition)		Medium: f/2 Temperature: 20 C Light intensity: 21 µmol photons/m²/sec, L/D cycle: 10L:14D Duration: 1 M
Gene information		ITS1-5.8S-ITS2 ( AB334314 ) , CO1 ( AB334451 ) , rbcL ( AB334378 ) , CO1 ( AB445408 ) , 18S-ITS1-5.8S-ITS2-28S ( AB286914 ) , CO2, CO1 ( AB286882 )
Cell size (min - max)		50 - 100 μm
Organization		Unicellular; Flagellate
Characteristics		Red tide
Other strain no.		Other strain no. : Hiroshima-70
Remarks		Fragile species to transportation stresses; Read and agree "How to order 4.1"
Movie

Reference
Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108. Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518 DOI: 10.1111/pre.12047 Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375. Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429 PubMed: 19346162 DOI: 10.1016/j.protis.2009.02.003 Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535. Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 1986, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407 DOI: 10.2216/08-98.1 Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880. Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429 DOI: 10.1111/j.1444-2906.2007.01408.x Hara, Y., Doi, K., Chihara, M. 1994 Four new species of Chattonella (Raphidophyceae, Chromophyta) from Japan. Jpn. J. Phycol. (Sôrui), 42, 407-420. Keywords: Chattonella; C. globosa; C. minima; C. ovata; C. verruculosa; Raphidophyceae; red tide organisms; taxonomy Strain(s): 118, 161, 603, 670, 849 Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227. Strain(s): 5, 15, 161 DOI: 10.2216/i0031-8884-28-2-222.1 Hara, Y. & Chihara, M. 1982 Ultrastructure and taxonomy of Chattonella (Class Raphidophyceae) in Japan. Jpn. J. Phycol. (Sôrui), 30, 47-56 (in Japanese with English summary). Keywords: Chattonella; Chattonella marina comb. nov.; Raphidophyceae; red tides; taxonomy; ultrastructure Strain(s): 161

Reference

Demura, M., Nakayama, T., Kasai, F., Kawachi, M. 2014 Genetic structure of Japanese Chattonella marina (Raphidophyceae) populations revealed using microsatellite markers. Phycol. Res., 62, 102-108.
Keywords: Chattonella marina var. antiqua; Chattonella marina var. marina; genetic structure; isolation by distance; microsatellite marker; population genetics
Strain(s): 1, 2, 3, 14, 83, 84, 85, 86, 113, 114, 115, 116, 118, 121, 161, 557, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2390, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407, 2509, 2510, 2511, 2512, 2513, 2514, 2515, 2516, 2517, 2518
DOI: 10.1111/pre.12047

Kamikawa, R., Masuda, I., Demura, M., Oyama, K., Yoshimatsu, S., Kawachi, M., Sako, Y. 2009 Mitochondrial group II introns in the Raphidophycean flagellate Chattonella spp. suggest a diatom-to-Chattonella lateral group II intron transfer. Protist, 160, 364-375.
Keywords: Chattonella; cytochrome c oxidase subunit 1 (cox1); diatoms; group II intron; lateral transfer; Raphidophyceae
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 462, 557, 558, 603, 671, 716, 849, 1303, 1975, 1976, 2391, 2401, 2402, 2403, 2404, 3422, 3429
PubMed: 19346162
DOI: 10.1016/j.protis.2009.02.003

Demura, M., Noël, M.-H., Kasai, F., Watanabe, M. M., Kawachi, M. 2009 Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia, 48, 518-535.
Keywords: Chattonella antiqua; Chattonella marina; Chattonella ovata; COI; COX1; Genetic diversity; ITS; Microsatellite; Morphology; Phylogeny; Population; Raphidophyceae; rbcL; Speciation; Taxonomy
Strain(s): 1, 2, 3, 5, 6, 9, 10, 14, 83, 84, 85, 113, 114, 116, 118, 146, 161, 293, 557, 561, 603, 671, 716, 849, 1974, 1975, 1976, 1977, 1978, 1980, 1981, 1982, 1983, 1984, 1986, 2383, 2384, 2385, 2386, 2387, 2388, 2389, 2391, 2392, 2393, 2394, 2395, 2396, 2397, 2398, 2399, 2400, 2401, 2402, 2403, 2404, 2405, 2406, 2407
DOI: 10.2216/08-98.1

Kamikawa, R., Masuda, I., Oyama, K., Yoshimatsu, S., Sako, Y. 2007 Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae Chattonella species. Fish. Sci., 73, 871-880.
Keywords: Chattonella sp.; cytochrome c oxidase subunit 1 (cox1); cytochrome c oxidase subunit 2 (cox2); genetic diversity; harmful algae; mitochondria
Strain(s): 1, 2, 3, 14, 85, 86, 115, 118, 121, 161, 557, 558, 603, 671, 849, 3422, 3428, 3429
DOI: 10.1111/j.1444-2906.2007.01408.x

Hara, Y., Doi, K., Chihara, M. 1994 Four new species of Chattonella (Raphidophyceae, Chromophyta) from Japan. Jpn. J. Phycol. (Sôrui), 42, 407-420.
Keywords: Chattonella; C. globosa; C. minima; C. ovata; C. verruculosa; Raphidophyceae; red tide organisms; taxonomy
Strain(s): 118, 161, 603, 670, 849

Kawai, H., Inouye, I. 1989 Flagellar autofluorescence in forty-four chloraphyll c-containig algae. Phycologia, 28, 222-227.
Strain(s): 5, 15, 161
DOI: 10.2216/i0031-8884-28-2-222.1

Hara, Y. & Chihara, M. 1982 Ultrastructure and taxonomy of Chattonella (Class Raphidophyceae) in Japan. Jpn. J. Phycol. (Sôrui), 30, 47-56 (in Japanese with English summary).
Keywords: Chattonella; Chattonella marina comb. nov.; Raphidophyceae; red tides; taxonomy; ultrastructure
Strain(s): 161

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