Strain number NIES-1642  
Phylum Cyanophyta  
Class Cyanophyceae  
Scientific name Dolichospermum affine (Lemmermann) Wacklin, Hoffmann & Komárek  
Synonym Anabaena affinis Lemmermann; Anabaena catenula var. affinis (Lemmermann) Geitler 1932  
Former name Anabaena affinis Lemmermann  
Common name Blue-green alga ; Cyanobacteria  
Locality (Date of collection) Lake Tsukui Tsukui Kanagawa Japan  
Latitude / Longitude 35.5922557587 / 139.260394458 
Habitat (Isolation source) Freshwater (Lake water)  
History < TAC;  
Isolator (Date of isolation) Watanabe, Masayuki  
Identified by Watanabe, Masayuki  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  CB  
Temperature:  20 C
Light intensity:  10-19 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 16S rRNA ( AB551451 ) , rbcL ( AB551499 )  
Cell size (min - max) 5 - 8 μm  
Organization Filamentous 
Characteristics Planktonic  
Other strain no. Other strain no. : TAC 454  
Remarks  
Movie  
Reference
Asukabe, H., Akahori, S., Ueno, E., Nakayama, T., Yamashita, R., Arii, S., Harada, K., Imanishi, S. Y. 2020 Cyanobacterial classification with the toxicity using MALDI Biotyper. J. Am. Soc. Mass Spectrom., 31, 1572-1578.
Keywords: cyanobacteria; MALDI Biotyper; microcystins; profiling
Strain(s): 27394273818789100101102103104105107111112207208230266298416433478510512515592593604611808809835843846847905928931932945981989102510431058106110641068106910711076108510991101110511131115112611331134113511441157121516421643165216681672167316771678169820872131241226653708373337343736375537563757 
PubMed: 32501712
DOI: 10.1021/jasms.0c00148

Imanishi, S. Y., Nakayama, T., Asukabe, H., Harada, K. 2017 Application of MALDI Biotyper to cyanobacterial profiling. Rapid Commun. Mass Spectrom., 31, 325-332.
Strain(s): 738910210310711229861180880983584398910251043105810711099164216521672 
PubMed: 27862451
DOI: 10.1002/rcm.7793

Tuji, A., Niiyama, Y. 2010 Phylogenetic study by the morphological and molecular analyses of Japanese planktonic Anabaena species. Bull. Natl. Mus. Nat. Sci., Ser. B, 36, 71-80.
Keywords: Anabaena; Anabaena oumiana; culture, Japan, morphology, planktonic; rbcL; rbcX; rDNA; Sphaerospermum oumianum (M. Watanabe) Tuji et Niiyama comb. nov.
Strain(s): 807808820822826163916421643164616511652165316571658166516671668167216731674167716781683169016931694169616971875187818801884188718911894189519041909192019211922192919311934193919421950 

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