Strain number NIES-1390  
Phylum Excavata incertae sedis  
Class Incertae sedis  
Scientific name Tsukubamonas globosa Yabuki et al.  
Former name  
Common name  
Locality (Date of collection) Hyotaro-ike Pond, Tennoudai, Tsukuba, Ibaraki, Japan (2003-01-16)  
Latitude / Longitude 36.116587 / 140.09765 
Habitat (Isolation source) Freshwater (Lake water)  
History < TKB;  
Isolator (Date of isolation) Yubuki, Naoji (2003-03-02)  
Identified by Yabuki, Akinori  
State of strain Subculture; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  URO + YT (1/10) + Wheat  
Temperature:  20 C
Light intensity:  0 µmol photons/m2/sec,
Duration:  20 D  
Gene information 18S rRNA ( AB576851 ) , EF2 ( AB576859 ) , hsp90 ( AB576858 ) , beta-tubulin ( AB576857 AB576856 ) , alpha-tubulin ( AB576855 ) , actin ( AB576854 AB576853 AB576852 )  
Cell size (min - max)  
Characteristics Heterotrophic ; Planktonic ; Authentic strain (Yabuki et al. 2011)  
Other strain no. Other strain no. : TKB-055 (NY0136)  
Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 8254274286324331353381494623624997101210131015132013301333133413351372137713881390139613971408143814391441186819642008214221442149230023052325241124332498250225662584258625892590267728783374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Yabuki, A., Nakayama, T., Yubuki, N., Hashimoto, T., Ishida, K-I. Inagaki, Y. 2011 Tsukubamonas globosa n. gen., n. sp., a novel excavate flagellate possibly holding a key for the early evolution in "Discoba". J. Eucaryot. Microbiol., 58, 319-331.
Keywords: Flagellar apparatus; heteroloboseans; jakobids; multigene phylogeny; taxonomy; ultrastructure
Strain(s): 1390 
PubMed: 21569159
DOI: 10.1111/j.1550-7408.2011.00552.x

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