A big image opens when the thumbnail is clicked.(nies-1335.jpg)
Strain number NIES-1335  
Phylum Kathablepharida  
Class Kathablepharidea  
Scientific name Leucocryptos marina (Braarud) Butcher  
Former name  
Common name  
Locality (Date of collection) Tokyo Bay Japan (2003-10-16)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Seawater)  
History < TKB;  
Isolator (Date of isolation) Okamoto, Noriko (2003-12-**)  
Identified by Okamoto, Noriko  
State of strain Subculture; Mixed (Chrysochromulina sp. NIES-1333 as food); Non-clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  mIMR  
Temperature:  20 C
Light intensity:  4-5 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information Mitochondrial DNA ( LC515368 ) , 18S rRNA ( AB194980 DQ980480 DQ980481 ) , LSU rRNA ( DQ980471 ) , actin ( DQ980424 DQ980425 DQ980426 DQ980439 DQ980440 DQ980441 ) , alpha-tubulin ( DQ980496 DQ980535 DQ980537 DQ980538 ) , beta-tubulin ( AB194977 AB194978 DQ980511 FJ973420 ) , eEF-2 ( EU812190 EU812191 EU812192 EU812193 EU812194 EU812195 ) , Hsp70 ( EU812178 EU812179 EU812180 EU812181 EU812182 ) , Hsp90 ( DQ980461 DQ980462 DQ980463 ) , nad11 ( AB693966 )  
Cell size (min - max) - 15 μm  
Organization Unicellular; Flagellate 
Characteristics Heterotrophic ; Phagotrophic ; Genome decoded strain (Nishimura et al. 2020)  
Other strain no. Other strain no. : TKB-081 (nrc057)  
Nishimura. Y., Kume, K., Sonehara, K., Tanifuji, G., Shiratori, T., Ishida, K-I., Hashimoto, T., Inagaki, Y., Ohkuma, M. 2020 Mitochondrial genomes of Hemiarma marina and Leucocryptos marina revised the evolution of cytochrome c maturation in Cryptista. Front. Ecol. Evol., 8, 140 (article ID).
Keywords: kathablepharids; cytochrome c maturation; goniomonads; cryptophytes; holocytochrome c synthase
Strain(s): 1335 
DOI: 10.3389/fevo.2020.00140

Kashiyama, Y. et al. 2019 Taming chlorophylls by early eukaryotes underpinned algal interactions and the diversification of the eukaryotes on the oxygenated Earth. ISME J., 13, 1899-1910.
Strain(s): 82542742863243313533814946236249971012101310151320133013331334133513721377138813901396139714081438143914411868196420082142214421492300230523252411243324982502256625842586258925902677287833563374360437753876 
PubMed: 30809012
DOI: 10.1038/s41396-019-0377-0

Nishimura, Y. 2016 Diversity and evolution of protist mitochondria : introns, gene content and genome architecture. PhD thesis, University of Tsukuba, Japan, 75 pp.
Strain(s): 13331335 

Nishimura, Y., Kamikawa, R., Hashimoto, T., Inagaki, Y. 2012 Separate origins of group I introns in two mitochondrial genes of the katablepharid Leucocryptos marina. PLoS One, 7, e37307 (article ID).
Strain(s): 13331335 
PubMed: 22606358
DOI: 10.1371/journal.pone.0037307

Reeb, V. C., Peglar, M. T., Yoon, H. S., Bai, J. R., Wu, M., Siu, P., Grafenberg, J. L., Reyes-Prieto, A., Rummele, S. E., Gross, J., Bhattacharya, D. 2009 Interrelationships of chromalveolates within a broadly sampled tree of photosynthetic protists. Mol. Phylogenet. Evol., 53, 202–211.
Keywords: Chromalveolata; Multigene phylogenetics; Plastidophila; Rhizaria; Supergroup; Tree of life
Strain(s): 13341335 
PubMed: 19398025
DOI: 10.1016/j.ympev.2009.04.012

Kim, E., Graham, L. E. 2008 EEF2 analysis challenges the monophyly of Archaeplastida and Chromalveolata. PLoS One, 3, e0002621 (article ID).
Strain(s): 1335 
PubMed: 18612431
DOI: 10.1371/journal.pone.0002621

Kim, E., Simpson, A. G., Graham, L. E. 2006 Evolutionary relationships of apusomonads inferred from taxon-rich analyses of nuclear encoded genes. Mol. Biol. Evol., 23, 2455-2466.
Keywords: Apusomonas; Apusomonadidae; Opisthokonta; Amoebozoa; protists; evolution
Strain(s): 93695513331335 
PubMed: 16982820
DOI: 10.1093/molbev/msl120

Okamoto, N. & Inouye, I. 2005 The katablepharids are a distant sister group of the Cryptophyta: a proposal for Katablepharidophyta divisio nova/Kathablepharida phylum novum based on SSU rDNA and beta-tubulin phylogeny. Protist, 156, 163-179.
Keywords: beta-tubulin; Cryptophyta; Katablepharidophyta/Kathablepharida; Katablepharis/Kathablepharis japonica; Leucocrypta; Leucocryptos marina; phylogeny; SSU rDNA
Strain(s): 133313341335 
PubMed: 16171184
DOI: 10.1016/j.protis.2004.12.003

Unauthorized copying and replication of text, images, and tables in our homepage, are prohibited.