Strain number NIES-1289  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Trebouxia higginsiae (Hildreth & Ahmadjian) Gärtner  
Former name  
Common name Green alga  
Locality (Date of collection) Tsukuba-san Shrine, Tsukuba, Ibaraki, Japan (2002-01-19)  
Latitude / Longitude  
Habitat (Isolation source) Terrestrial (The lichen Parmotrema tinctorum on bark)  
History < Ohmura, Yoshihito  
Isolator (Date of isolation) Ohmura, Yoshihito (2002-02-15)  
Identified by Takeshita, Shunji  
State of strain Cryopreservation; Unialgal; Clonal; Axenic  
Culture condition
(Preculture condition)
Medium:  C (agar)  
Temperature:  20 (25) C
Light intensity:  7-13 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  6 M (21 D)  
Gene information ITS1-5.8S-ITS2-28S ( MK328540 ) , 28S rRNA ( MK320924 ) , CO2 ( MK314971 ) , rbcL ( MK314947 )  
Cell size (min - max)  
Organization Unicellular 
Characteristics Symbiotic (lichen)  
Other strain no. Other strain no. : AYO4781  
Remarks Cryopreserved; Axenic 
Xu, M., de Boer, H., Olafsdottir, E. S., Omarsdottir, S., Heidmarsson, S. 2020 Phylogenetic diversity of the lichenized algal genus Trebouxia (Trebouxiophyceae, Chlorophyta): a new lineage and novel insights from fungal-algal association patterns of Icelandic cetrarioid lichens (Parmeliaceae, Ascomycota). Bot. J. Linnean Soc., 194, 460–468.
Keywords: Iceland; lichen; Parmeliaceae; phylogeny; symbiosis; Trebouxia
Strain(s): 127112781289 
DOI: 10.1093/botlinnean/boaa050

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