Strain number NIES-1269  
Phylum Chlorophyta  
Class Trebouxiophyceae  
Scientific name Chlorella vulgaris Beijerinck  
Synonym  
Former name Formerly identified as Chlorella sp., re-identified at NIES by DNA analysis  
Common name Green alga  
Locality (Date of collection) Banzu Tidal Flat, Kisarazu, Chiba, Japan (2002-05-14)  
Latitude / Longitude  
Habitat (Isolation source) Marine (Tidal flat water)  
History < Moriya, Mayumi  
Isolator (Date of isolation) Moriya, Mayumi (2002-06-18)  
Identified by Re-identified by NIES  
State of strain Cryopreservation; Unialgal; Clonal; Non-axenic  
Culture condition
(Preculture condition)
Medium:  ESM  
Temperature:  20 C
Light intensity:  22-32 µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  1 M  
Gene information 18S rRNA ( AB488579 )  
Cell size (min - max) 10 - 15 μm  
Organization Unicellular 
Characteristics  
Other strain no. Other strain no. : M-56  
Remarks Cryopreserved 
Movie  
Reference
Takahashi, M., Karitani, Y., Yamada, R., Matsumoto, T., Ogino, H. 2024 Co-utilization of microalgae and heterotrophic microorganisms improves wastewater treatment efficiency. Appl. Microbiol. Biotechnol., 108, 468 (article ID).
Keywords: Green algae; Heterotrophic microorganisms; Microalgae; Wastewater treatment; Yeast
Strain(s): 391269 
PubMed: 39292263
DOI: 10.1007/s00253-024-13309-w

Purba, L. D. A., Othman, A., Yuzir, A., Mohamad, S. V., Iwamoto, K., Abdullah, N., Shimizu, K., Hermana, J. 2022 Enhanced cultivation and lipid production of isolated microalgae strains using municipal wastewater. Environ. Technol. Innov., 27, 102444 (article ID).
Keywords: Microalgae; Municipal wastewater; Nutrient removal; Lipid production
Strain(s): 1269 
DOI: 10.1016/j.eti.2022.102444

Othman, F. S., Jamaluddin, H., Ibrahim, Z., Hara, H., Yahya, N. A., Iwamoto, K., Mohamad, S. E. 2019 Production of α-linolenic acid by an oleaginous green algae Acutodesmus obliquus isolated from Malaysia. J. Pure Appl. Microbiol., 13, 1297-1306.
Keywords: Acutodesmus obliquus; Chlorella sp.; Chlorella vulgaris; Lipid; Malaysia
Strain(s): 1269 
DOI: 10.22207/JPAM.13.3.01

Jo, B.-H., Lee, C. S., Song, H.-R., Lee, H.-G., Oh, H.-M. 2014 Development of novel microsatellite markers for strain-specific identification of Chlorella vulgaris. J. Microbiol. Biotechnol., 24, 10.3354/ame01939
Keywords: Chlorella vulgaris; chloroplast; microsatellite; marker; polymorphism; sequence repeat
Strain(s): 64164268612692170 
PubMed: 24931503
DOI: 10.4014/jmb.1405.05047

Sharifah, E. N. & Eguchi, M. 2012 Benefits of live phytoplankton, Chlorella vulgaris, as a biocontrol agent against fish pathogen Vibrio anguillarum. Fish. Sci., 78, 367-373.
Keywords: Chlorella vulgaris; Vibrio anguillarum; Biocontrol; Sulfitobacter sp.
Strain(s): 1269 
DOI: 10.1007/s12562-011-0465-1

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