Strain number NIES-49  
Phylum Euglenozoa  
Class Euglenophyceae  
Scientific name Euglena gracilis Klebs  
Former name Euglena gracilis var. bacillaris Pringsheim  
Common name  
Locality (Date of collection)  
Latitude / Longitude  
Habitat (Isolation source)  
History < IAM (1983);  
Isolator (Date of isolation)  
Identified by  
State of strain Subculture; Unialgal; Clonal; Axenic[2017 Dec]  
Culture condition
(Preculture condition)
Medium:  HUT  
Temperature:  20 (25) C
Light intensity:  2 (14-16) µmol photons/m2/sec, L/D cycle:  10L:14D
Duration:  45 D (14 D)  
Gene information  
Cell size (min - max) 40 - 50 μm  
Organization Unicellular; Flagellate 
Other strain no. Other collection strain no. : IAM E-2 (=E-10)
Remarks Axenic 
Okamoto, Y., Haraguchi, Y., Sawamura, N., Asahi, T., Shimizu, T. 2020 Mammalian cell cultivation using nutrients extracted from microalgae. Biotechnol. Prog., 36, e2941 (article ID).
Keywords: acidic hydrolysis; amino acids; glucose; mammalian cell culture; microalgae; nutrients
Strain(s): 2739492170 
PubMed: 31756286
DOI: 10.1002/btpr.2941

Demura, M. 2020 Biomass productivity and paramylon content of 3 strains of Euglena gracilis. Bull. Fac. Agr., Saga Univ., 105, 1-7 (in Japanese with English summary).
Keywords: Euglena; paramylon; biomass productivity; cost
Strain(s): 474849 

Nakamura, M., Yamagiwa, Y., HigashiuraA, N., Tokino, S., Maeda, T. 2018 Fundamental study on application of the newly isolated Euglena to wastewater treatment. Jpn. J. Water Treat. Biol., 54, 39-46 (in Japanese with English summary).
Keywords: Euglena; growth rate; wastewater treatmen
Strain(s): 474849 
DOI: 10.2521/jswtb.54.39

Aoyama, K., Miyata, N., Iwahori, K. 2005 Evalution of DNA damaging potential in landfill leachates by comet assay using Euglena gracilis. Jpn. J. Water Treat. Biol., 41, 73-81.
Keywords: comet assay; Euglena gracilis; landfill leachate; umu test
Strain(s): 49 

Iwahori, K., Miyata, N., Yamamoto, Y., Sone, D., Aoyama, K. 2004 Normalization of DNA damage indices in comet assay for comparing with various mutagenicity tests. Jpn. J. Water Treat. Biol., 40, 63-70.
Keywords: comet assay; tail moment; normalization; mutagenicity test
Strain(s): 49 

Aoyama, K., Iwahori, K., Miyata, N. 2003 Application of Euglena gracilis cells to comet assay: evaluation of DNA damage and repair. Mutat. Res. Genet. Toxicol. Environ. , 538, 155-162.
Keywords: Comet assay; Single-cell gel electrophoresis; Euglena gracilis; DNA damage; DNA repair
Strain(s): 49 
DOI: 10.1016/S1383-5718(03)00113-X

Iwahori, K., Miyata, N., Umeda, Y., Aoyama, K., Shimoi, K., Kinae, N. 1999 Applicability of environmental microorganisms to alkaline single cell gel electrophoresis (comet) assay. Jpn. J. Water Treat. Biol., 35, 261-270 (in Japanese with English summary).
Keywords: comet assay; DNA damage; environmental microorganisms; Euglena gracilis; cell lysis; unwinding; electrophoresis; SDS; γ-irradiation
Strain(s): 49122403 

Iwata, H., Haji, A., Hashimoto, K., Mitsuyanagi, K., Kanegae, Y. 1991 Methods for producing heat-unsoluble β-1,3-glucan [Kanetsu gyôkosei β-1,3-glucan no seizôhô]. In Nippon-koku Tokkyo-chô (JP), Kôkai Tokkyo Kôhô (A), Hei3-163102, 5 pp. (in Japanese without English title).
Strain(s): 474849 

Ichimura, T., Itoh, T. 1977 17. Preservation methods of microalgae (I) [17. Bisaisôrui no hozonhô (I)]. In Preservation methods of microorganisms [Biseibutsu Hozonhô], Ed. by Nei, T., University of Tokyo Press, Tokyo, pp. 355-373 (in Japanese without English title).
Strain(s): 19212223242728303132333436373839404144454647484950122123127131144152173175179180181182183185198200203206217226227241266267268271295309310334338379436448450455527 

Watanabe, A. 1960 List of algal strains in collection at the Institute of Applied Microbiology, University of Tokyo. J. Gen. Appl. Microbiol., 6, 283-292.
Strain(s): 192122232425262829374749226227 

Nishimura, M., Huzisige, H. 1959 Studies on the chlorophyll formation in Euglena gracilis with special reference to the action spectrum of the process. J. Biochem., 46, 225-234.
Strain(s): 49 

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